308-R
308-R is a SGK3/SGK1/S6K1 inhibitor. 308-R exhibits an IC50 of 5 nM against human SGK3, an IC50 of 10 nM against human SGK1, and an IC50 of 1 nM against human S6K1. 308-R can be used in breast cancer research. It is also applicable to studies related to PROTAC synthesis, such as serving as the target protein ligand for PROTAC SGK3 degrader-2 (HY-176823).
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- CAS No.: 2376724-98-8
- 화학식: C23H23FN6O4S
- 분자량:498.53
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보관:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocol
- 순도&문서
- References
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Help & FAQs
Biological Activity
제품 설명
IC50 & Target
[1]|
SGK3 5 nM (IC50) |
SGK1 10 nM (IC50) |
S6K1 1 nM (IC50) |
In Vitro
308-R potently inhibits SGK3, SGK1, and S6K1 kinases in vitro with IC50 values of 5 nM, 10 nM, and 1 nM, respectively[1].
308-R (1 μM; 8 h) potently suppresses NDRG1 Thr346 phosphorylation in HEK293 cells when combined with Akt inhibition, while 308-R (1 μM; 8 h) has minimal effect under basal conditions[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:HEK293 cells
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Concentration:1 μM (basal conditions); 1 μM (combination with Akt inhibition)
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Incubation Time:8 h (basal conditions); 8 h (combination with Akt inhibition)
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Result:Exerted minimal effect on phosphorylation of NDRG1 at Thr346 under basal conditions without Akt inhibition.
Markedly suppressed phosphorylation of NDRG1 at Thr346 in the presence of Akt inhibition.
Chemical Information
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CAS No. 2376724-98-8
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분자량 498.53
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화학식 C23H23FN6O4S
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SMILES
CC1=CC=C(C(S(=O)(NC2=CC=C(C=C2)C3=NC(OC[C@H]4CNCCO4)=C5C=NNC5=N3)=O)=C1)F
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocol
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Breast Cancer Modeling
Breast cancer is a heterogeneous cancer, and it has been distinguished into four subtypes: luminal A, luminal B, HER2-positive and basal-like. Molecular mutations, epigenetic alterations, hormone exposure and immune microenvironment are related to the progression of breast cancer.
순도&문서
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
308-R Related Classifications
HY-151009 Related Classifications
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