APO-15
Based on 1 Customer Validation
APO-15 is a phosphatidylserine-binding fluorescent probe and apoptosis imaging reagent. APO-15 exhibits high chemical stability under proteolytic and oxidative conditions, enables quantification and imaging of drug-induced apoptosis in preclinical mouse models, and is applicable to fixed tissue samples and multiple in vivo administration routes (Ex = 488 nm; Em = 525 nm). APO-15 can be used in studies related to acute lung injury and breast cancer.
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- Purity: 99.45%
- CAS No.: 3051946-11-0
- 화학식: C70H85BF2N16O7
- 분자량:1311.33
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보관:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Biological Activity
Apo-15 (50 μM; 24 h) exhibits high chemical stability under proteolytic, oxidative, and physiological buffer conditions, with >95% of the peptide remaining intact after 24 h exposure[1].
Apo-15 (100 nM; 10 min, 10 min at room temperature) competes with AF647-Annexin V for binding sites on apoptotic human neutrophils, confirming PS as its molecular target[1].
Apo-15 (100 nM; 10 min at room temperature) binding to apoptotic human cells is dependent on externalized PS, as it fails to stain apoptotic cells that cannot translocate PS to the outer membrane[1].
Apo-15 (100 nM; 15 min, 1000 s) enables wash-free, real-time imaging of apoptotic progression in live human lymphoid cells, with rapid fluorescence activation upon PS exposure[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
APO-15 (5 μM; i.v.; single administration) detects cisplatin-induced apoptosis in mouse MMTV-PyMT breast cancer tumors, with signal co-localizing with the apoptotic marker cleaved caspase-3[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6 (8-12 weeks old; induced by intratracheal instillation of E. coli-derived LPS, S. aureus-derived LTA and PepG)[1]
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Dosage:5 μM
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Administration:intratracheal; single administration
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Result:Showed 29% of CD45+Ly6G+ neutrophils in BALF stained positive, compared to 5% in LPS-only treated mice. Detected 90 APO-15-positive cells per 0.25 μm2 section in lung tissue from LPS + CDKi-treated mice, compared to 32 cells per 0.25 μm2 section in LPS-only treated mice. Demonstrated negligible staining in healthy saline-injected control mice, and bright, stable staining in the lungs of mice with ALI.
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Animal Model:MMTV-PyMT (C57BL/6 background; tumors grown to 0.5-1 cm size)[1]
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Dosage:5 μM
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Administration:i.v.; single administration
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Result:Retained signal in tumor tissues after OCT embedding and sectioning. Co-localized with staining for cleaved caspase-3, confirming detection of apoptotic cells.
Chemical Information
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CAS No. 3051946-11-0
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Appearance Solid
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분자량 1311.33
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화학식 C70H85BF2N16O7
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Color Orange to red
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
용액&용해도
DMSO : 50 mg/mL (38.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (1.91 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (1.91 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (286 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.7626 mL | 3.8129 mL | 7.6258 mL | 19.0646 mL |
| 5 mM | 0.1525 mL | 0.7626 mL | 1.5252 mL | 3.8129 mL | |
| 10 mM | 0.0763 mL | 0.3813 mL | 0.7626 mL | 1.9065 mL | |
| 15 mM | 0.0508 mL | 0.2542 mL | 0.5084 mL | 1.2710 mL | |
| 20 mM | 0.0381 mL | 0.1906 mL | 0.3813 mL | 0.9532 mL | |
| 25 mM | 0.0305 mL | 0.1525 mL | 0.3050 mL | 0.7626 mL | |
| 30 mM | 0.0254 mL | 0.1271 mL | 0.2542 mL | 0.6355 mL |
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