BRD2577
Based on 1 Customer Validation
BRD2577 is a selective mercaptopyruvate sulfurtransferase (3-MST) inhibitor with an IC50 of 9.9 μM. BRD2577 inhibits H2S production in colon carcinoma cells. BRD2577 exhibits weak to no significant inhibitory effect on colon cancer cell proliferation and mitochondrial activity. BRD2577 can be used for the research of colon cancer.
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- Purity: 98.22%
- CAS No.: 353781-42-7
- 화학식: C15H15N3O3S
- 분자량:317.36
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보관:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Biological Activity
제품 설명
IC50 & Target
[1]|
MST3 9.9 μM (IC50) |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CT26 | IC50 |
>1 mM
Compound: 13a
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Antiproliferative activity against mouse CT26 cells assessed as inhibition of cell proliferation measured after 24 hrs by MTT assay
Antiproliferative activity against mouse CT26 cells assessed as inhibition of cell proliferation measured after 24 hrs by MTT assay
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[PMID: 33856792] |
| CT26 | IC50 |
1000 μM
Compound: 13a
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Antiproliferative activity against mouse CT26 cells assessed as inhibition of cell proliferation measured after 24 hrs by MTT assay
Antiproliferative activity against mouse CT26 cells assessed as inhibition of cell proliferation measured after 24 hrs by MTT assay
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[PMID: 33856792] |
| MC-38 | IC50 |
>1 mM
Compound: 13a
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Antiproliferative activity against mouse MC38 cells assessed as inhibition of cell proliferation measured after 24 hrs by MTT assay
Antiproliferative activity against mouse MC38 cells assessed as inhibition of cell proliferation measured after 24 hrs by MTT assay
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[PMID: 33856792] |
In Vitro
BRD2577 (Compound 13a) (3-300 μM; 1 h) potently inhibits recombinant human 3-MST with an IC50 of 9.9 μM and achieves ≥60% inhibition at 100 μM, and shows no activity against human recombinant CBS or CSE at concentrations up to 300 μM[1].
BRD2577 (300 μM; 2 h) does not act as an H2S scavenger at 300 μM, confirming its inhibitory effect is direct against 3-MST[1].
BRD2577 (300 μM; 4 h) inhibits H2S production in live murine CT26 and MC38 colon carcinoma cells[1].
BRD2577 (3-300 μM; 48 h) does not significantly inhibit the proliferation of murine CT26 or MC38 colon carcinoma cells at concentrations up to 300 μM after 48 h of treatment[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Murine CT26 colon carcinoma cells, murine MC38 colon carcinoma cells
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Concentration:3, 10, 30, 100, 300 μM
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Incubation Time:48 h
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Result:Did not significantly inhibit CT26 or MC38 cell proliferation at tested concentrations.
Showed an IC50 values were >1000 μM for both cell lines.
Chemical Information
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CAS No. 353781-42-7
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Appearance Solid
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분자량 317.36
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화학식 C15H15N3O3S
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Color Light yellow to yellow
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SMILES
O=C1N=C(SCC(=O)C2=CC=C(C=C2)NC(=O)C)NC(=C1)C
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
용액&용해도
In Vitro:
DMSO : 100 mg/mL (315.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.88 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.88 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1510 mL | 15.7550 mL | 31.5100 mL | 78.7749 mL |
| 5 mM | 0.6302 mL | 3.1510 mL | 6.3020 mL | 15.7550 mL | |
| 10 mM | 0.3151 mL | 1.5755 mL | 3.1510 mL | 7.8775 mL | |
| 15 mM | 0.2101 mL | 1.0503 mL | 2.1007 mL | 5.2517 mL | |
| 20 mM | 0.1575 mL | 0.7877 mL | 1.5755 mL | 3.9387 mL | |
| 25 mM | 0.1260 mL | 0.6302 mL | 1.2604 mL | 3.1510 mL | |
| 30 mM | 0.1050 mL | 0.5252 mL | 1.0503 mL | 2.6258 mL | |
| 40 mM | 0.0788 mL | 0.3939 mL | 0.7877 mL | 1.9694 mL | |
| 50 mM | 0.0630 mL | 0.3151 mL | 0.6302 mL | 1.5755 mL | |
| 60 mM | 0.0525 mL | 0.2626 mL | 0.5252 mL | 1.3129 mL | |
| 80 mM | 0.0394 mL | 0.1969 mL | 0.3939 mL | 0.9847 mL | |
| 100 mM | 0.0315 mL | 0.1575 mL | 0.3151 mL | 0.7877 mL |
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