DCG04
Based on 1 publication(s) in Google Scholar
DCG04 is a biotinylated activity-based probe. DCG04 labels calpain-1 and is applicable for affinity purification and mass spectrometry identification. DCG04 is used in cancer and malaria research.
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- Purity : 98.55%
- CAS No.: 314263-42-8
- 화학식: C43H66N8O11S
- 분자량:903.10
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보관:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) DCG04
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Biological Activity
제품 설명
In Vitro
DCG04 reveals a progressive increase in cathepsin activity during RIP1-Tag2 tumorigenesis, identifying cathepsins B, C, L, and Z as active proteases in tumors[1].
DCG04 profiling of flow-sorted RIP1-Tag2 tumor cells shows that infiltrating innate immune cells are a significant source of cathepsin activity, with distinct activity profiles among endothelial, immune, and tumor cell fractions[1].
DCG-04 (50 μM) is an activity-dependent affinity label that covalently modifies multiple active cysteine proteases in DC2.4 dendritic cell extracts at pH 5.5[2].
DCG-04 affinity labeling enriches a 27-kDa cysteine protease from bovine chromaffin granules that is responsible for PE-processing activity[3].
DCG04 activity labeling confirms that immunodepletion and calpastatin loading abolish calpain-1 activity in resealed erythrocytes, which is restored by calpain-1 reconstitution[8].
DCG04 (4 μM; 4 h) is a component of the biotinylated probe cocktail used for ABPP-MS to display active cysteine proteases in N. benthamiana apoplastic fluid, revealing that bacterial infection reduces the activity of nine cysteine proteases[11].
DCG04 (~48 hours postinfection) labels a single ~80 kD membrane-associated protein in P. falciparum-infected erythrocytes, identified as human calpain-1[8].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 314263-42-8
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Appearance Solid
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분자량 903.10
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화학식 C43H66N8O11S
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Color White to off-white
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SMILES
OC(C=C1)=CC=C1C[C@@H](C(NCCCCCC(N[C@H](C(N)=O)CCCCNC(CCCC[C@H]2[C@]3([H])[C@](NC(N3)=O)([H])CS2)=O)=O)=O)NC([C@H](CC(C)C)NC([C@@H]4[C@H](O4)C(OCC)=O)=O)=O
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
용액&용해도
In Vitro:
DMSO : 3.33 mg/mL (3.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
순도&문서
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Data Sheet (273 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.1073 mL | 5.5365 mL | 11.0730 mL | 27.6824 mL |
DCG04 Related Classifications
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