Kudinoside D
Based on 1 publication(s) in Google Scholar
Kudinoside D is a triterpenoid saponin. Kudinoside D is isolated from the leaves of Ilex kudingcha. Kudinoside D enhances the phosphorylation levels of AMPK and its downstream molecule ACC, and inhibits the expression of PPARγ, C/EBPα, SREBP-1c as well as the target genes of adipogenic transcription factors. Kudinoside D inhibits the activity of ANO1. Kudinoside D possesses hypolipidemic activity. Kudinoside D can be used in obesity-related research.
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- Pureza : 99.80%
- No. CAS: 173792-61-5
- Fòrmula: C47H72O17
- Peso molecular:909.06
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Almacenamiento:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Kudinoside D
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Actividad biológica
Descripciòn
IC50 & Target
[1]|
PPARγ |
In Vitro
Kudinoside D (0-40 μM; 7 days) shows no cytotoxicity against 3T3-L1 preadipocytes, with a cell viability IC50 of 59.49 μM[1].
Kudinoside D (30 μM) inhibits ANO1 activity in FRT cells expressing ANO1 and YFP, with an inhibition rate of 26.2% at the concentration of 30 μM[2].
Kudinoside D (20-40 μM; 7 days) dose-dependently inhibits lipid droplet accumulation in differentiated 3T3-L1 adipocytes[1].
Treatment with Kudinoside D (20-40 μM; 7 days) throughout the 7-day differentiation period of 3T3-L1 adipocytes reduces intracellular triglyceride levels in differentiated cells: a 32.4% reduction at 20 μM and a 61.6% reduction at 40 μM[1].
Kudinoside D (20-40 μM; 7 days) alters the fatty acid composition of differentiated 3T3-L1 adipocytes and reduces SCD-1 activity (evidenced by a decreased C18:1 n-9/C18:0 ratio), with this treatment applied throughout the 7-day differentiation process[1].
Kudinoside D (20-40 μM) dose-dependently downregulates the protein expression levels of key adipogenic transcription factors (C/EBPβ, SREBP-1c, PPARγ, C/EBPα) and their target proteins (FABP4, FAS) during adipogenic differentiation of 3T3-L1 cells[1].
Kudinoside D (40 μM; 24 h) suppresses the protein expression of PPARγ and C/EBPα in differentiated 3T3-L1 adipocytes in vitro in an AMPK-dependent manner, as this effect is blocked by pretreatment with the AMPK inhibitor Compound C (10 μM; 30 min)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:3T3-L1 preadipocytes
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Concentration:0-40 μM; 50 μM (cytotoxicity assessment)
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Incubation Time:7 days
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Result:Showed no cytotoxicity at concentrations up to 40 μM.
Exhibited a cell viability IC50 of 59.49 μM, with cytotoxicity starting around 50 μM.
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Cell Line:differentiated 3T3-L1 adipocytes
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Concentration:40 μM (kudinoside D); 10 μM (Compound C, pre-incubation)
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Incubation Time:24 h (kudinoside D); 30 min (Compound C, pre-incubation)
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Result:Inhibited the protein expression of PPARγ and C/EBPα without Compound C pretreatment.
Lost inhibitory effects on PPARγ and C/EBPα protein expression after pretreatment with Compound C.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 173792-61-5
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Appearance Solid
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Peso molecular 909.06
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Fòrmula C47H72O17
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Color White to off-white
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SMILES
CC1([C@H](CC[C@]2(C1CC[C@@]3(C2C=CC4=C5[C@@](C(O6)=O)(CC[C@@]34C)CC[C@]6([C@@]5(C)O)C)C)C)O[C@H]7[C@@H]([C@H]([C@H](CO7)O)O[C@H]8[C@@H]([C@H]([C@@H]([C@H](O8)CO)O)O)O)O[C@H]9[C@@H]([C@@H]([C@H]([C@@H](O9)C)O)O)O)C
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
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Journal Impact Factor
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Most Recent
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (110.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocolo
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Kinase activity and phosphorylation assays
Kinase activity assays measure the ability of kinases to transfer phosphate groups from ATP to specific substrates, while phosphorylation assays detect the presence and levels of phosphorylated proteins. Common methods include radiolabeled ATP incorporation (e. g. ,), ADP release detection via bioluminescence (e. g. ,[3]), enzyme-linked immunosorbent assays (ELISA) for phospho-specific epitopes (e. g. ,[6]), and microtiter-based formats for high-throughput screening (e. g. ,[8]). The ADP-Glo assay quantifies kinase activity by measuring ADP produced during phosphorylation using a luciferase-based system. Radiometric assays involve autoradiography or scintillation counting after incorporation of 32P-labeled ATP into substrate proteins. ELISA-based approaches rely on phospho-specific antibodies to detect activated kinases in cell lysates or purified samples.
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Western Blot
Western blotting (WB) is a commonly used experimental method in molecular biology, biochemistry, and immunogenetics for identifying and quantifying target proteins. It combines gel electrophoresis with immunoassay, enabling researchers to analyze protein expression, post-translational modifications, and molecular weight.
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Protocol for Kinase activity and phosphorylation assays
Kinase activity assays measure transfer of phosphate from ATP to a protein or peptide substrate, generating phosphorylated substrate, ADP, or incorporated radiolabeled phosphate as the readout; phosphorylation assays measure site-specific phosphorylation in cells or tissues as a proxy for kinase-pathway activation, inhibition, or substrate regulation. Phosphorylation can be detected by phospho-specific Western blot, immunoprecipitation kinase assay, phospho-immunofluorescence, phospho-flow cytometry, luminescent ADP detection, radiolabeled ATP incorporation, or reporter-based pathway assays, and these readouts can be applied to cancer cells, primary neurons, mouse tumors, organoids, inflammatory macrophages, ferroptosis studies, and mitophagy studies when the kinase target is biologically relevant.
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Research Protocol for Metabolic Diseases
AMP-activated protein kinase, AMPK, is a conserved cellular energy sensor that responds to reduced cellular energy status and coordinates metabolism by increasing ATP-generating catabolic pathways while suppressing ATP-consuming anabolic processes. In metabolic disease research, the AMPK pathway is experimentally relevant because it regulates hepatic lipid synthesis, fatty acid oxidation, glucose production, skeletal-muscle glucose disposal, mTORC1-linked biosynthesis, autophagy, mitochondrial homeostasis, and whole-body energy balance. The central pathway logic is that energy stress, metformin, exercise-like stimulation, or direct AMPK activators increase AMPKα Thr172 phosphorylation and downstream substrate phosphorylation, including ACC and RAPTOR. Phosphorylation of ACC suppresses lipogenesis and supports fatty acid oxidation, whereas phosphorylation of RAPTOR suppresses mTORC1 signaling and links cellular energy status to growth and protein synthesis control. The pathway is linked
Pureza y Documentación
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Ficha de datos (292 KB)
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SDS (252 KB)
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- Deutsch - DE (252 KB)
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- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Che Y, et al. Kudinoside-D, a triterpenoid saponin derived from Ilex kudingcha suppresses adipogenesis through modulation of the AMPK pathway in 3T3-L1 adipocytes. Fitoterapia. 2018 Mar;125:208-216. [Content Brief]
[2]. Mai NT, et al. Triterpenoid glycosides from the rhizomes of Allium ascalonicum and their anoctamin-1 inhibitory activity. Nat Prod Res. 2021 Nov;35(22):4338-4346. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.1000 mL | 5.5002 mL | 11.0004 mL | 27.5009 mL |
| 5 mM | 0.2200 mL | 1.1000 mL | 2.2001 mL | 5.5002 mL | |
| 10 mM | 0.1100 mL | 0.5500 mL | 1.1000 mL | 2.7501 mL | |
| 15 mM | 0.0733 mL | 0.3667 mL | 0.7334 mL | 1.8334 mL | |
| 20 mM | 0.0550 mL | 0.2750 mL | 0.5500 mL | 1.3750 mL | |
| 25 mM | 0.0440 mL | 0.2200 mL | 0.4400 mL | 1.1000 mL | |
| 30 mM | 0.0367 mL | 0.1833 mL | 0.3667 mL | 0.9167 mL | |
| 40 mM | 0.0275 mL | 0.1375 mL | 0.2750 mL | 0.6875 mL | |
| 50 mM | 0.0220 mL | 0.1100 mL | 0.2200 mL | 0.5500 mL | |
| 60 mM | 0.0183 mL | 0.0917 mL | 0.1833 mL | 0.4583 mL | |
| 80 mM | 0.0138 mL | 0.0688 mL | 0.1375 mL | 0.3438 mL | |
| 100 mM | 0.0110 mL | 0.0550 mL | 0.1100 mL | 0.2750 mL |
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