L-Asparagine-13C4,15N2 monohydrate
Based on 1 Customer Validation
L-Asparagine-13C4,15N2 monohydrateis the 13C-labeled and 15N-labeled L-Asparagine monohydrate (HY-W017443). L-Asparagine monohydrate is an essential amino acid for leukemic cells and a substrate for L-Asparaginase. L-Asparaginase is a potent anti-leukemic enzyme that promotes asparagine (Asn) and glutamine (Gln) depletion and inhibits protein biosynthesis in lymphoblasts. Removal of L-asparagine from plasma by L-Asparaginase results in inhibition of RNA and DNA synthesis and subsequent apoptosis. L-Asparaginase has cell-killing ability in vitro and in vivo, and selectively inhibits the growth of cancer cells with low asparagine synthetase (AASNS) expression. L-Asparagine monohydrate can be used as a biomarker and sensor for the study of childhood acute lymphoblastic leukemia.
For research use only. We do not sell to patients.
- Purity: 98%
- CAS No.: 202406-87-9
- Formula: 13C4H1015N2O4
- Molecular Weight:156.09
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Storage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Stable heavy isotopes of hydrogen, carbon, and other elements have been incorporated into drug molecules, largely as tracers for quantitation during the drug development process. Deuteration has gained attention because of its potential to affect the pharmacokinetic and metabolic profiles of drugs[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
1. This compound can be used as a tracer
2. This compound can be used as an internal standard for quantitative analysis by NMR, GC-MS, or LC-MS.
Chemical Information
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CAS No. 202406-87-9
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Unlabeled Cas 5794-13-8
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Appearance Solid
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Molecular Weight 156.09
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Formula 13C4H1015N2O4
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Color White to off-white
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SMILES
[15NH2][13C]([13CH2][13C@H]([15NH2])[13C](O)=O)=O.O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
H2O : 20 mg/mL (128.13 mM; Need ultrasonic)
DMSO : 1 mg/mL (6.41 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Russak EM, et al. Impact of Deuterium Substitution on the Pharmacokinetics of Pharmaceuticals. Ann Pharmacother. 2019;53(2):211-236. [Content Brief]
[2]. Batool T, et al. A Comprehensive Review on L-Asparaginase and Its Applications. Appl Biochem Biotechnol. 2016 Mar;178(5):900-23. [Content Brief]
[3]. Asselin BL, et al. In vitro and in vivo killing of acute lymphoblastic leukemia cells by L-asparaginase. Cancer Res. 1989 Aug 1;49(15):4363-8. [Content Brief]
[4]. Horvath TD, et al. Assessment of l-Asparaginase Pharmacodynamics in Mouse Models of Cancer. Metabolites. 2019 Jan 9;9(1):10. [Content Brief]
[5]. Kravtzoff R, et al. Erythrocytes as carriers for L-asparaginase. Methodological and mouse in-vivo studies. J Pharm Pharmacol. 1990 Jul;42(7):473-6. [Content Brief]
[6]. Ilaria M Michelozzi, et al. Acute myeloid leukaemia niche regulates response to L-asparaginase. Br J Haematol. 2019 Aug;186(3):420-430. doi: 10.1111/bjh.15920. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 6.4066 mL | 32.0328 mL | 64.0656 mL | 160.1640 mL |
| 5 mM | 1.2813 mL | 6.4066 mL | 12.8131 mL | 32.0328 mL | |
| H2O | 10 mM | 0.6407 mL | 3.2033 mL | 6.4066 mL | 16.0164 mL |
| 15 mM | 0.4271 mL | 2.1355 mL | 4.2710 mL | 10.6776 mL | |
| 20 mM | 0.3203 mL | 1.6016 mL | 3.2033 mL | 8.0082 mL | |
| 25 mM | 0.2563 mL | 1.2813 mL | 2.5626 mL | 6.4066 mL | |
| 30 mM | 0.2136 mL | 1.0678 mL | 2.1355 mL | 5.3388 mL | |
| 40 mM | 0.1602 mL | 0.8008 mL | 1.6016 mL | 4.0041 mL | |
| 50 mM | 0.1281 mL | 0.6407 mL | 1.2813 mL | 3.2033 mL | |
| 60 mM | 0.1068 mL | 0.5339 mL | 1.0678 mL | 2.6694 mL | |
| 80 mM | 0.0801 mL | 0.4004 mL | 0.8008 mL | 2.0021 mL | |
| 100 mM | 0.0641 mL | 0.3203 mL | 0.6407 mL | 1.6016 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
L-Asparagine-13C4,15N2 monohydrate Related Classifications
HY-W017443S Related Classifications
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