L-Cystine dihydrochloride
Based on 5 publication(s) in Google Scholar
L-Cystine dihydrochloride is the dihydrochloride salt form of L-Cystine (HY-N0394). L-Cystine dihydrochloride elevates Nrf2 protein expression and activates Nrf2 transcription factor. L-cystine dihydrochloride reduces ROS generation and protects against oxidant- or Doxorubicin (HY-15142A)-induced apoptosis. L-Cystine dihydrochloride combined with L-theanine (HY-15121) enhances the production of antigen-specific IgG by increasing glutathione (GSH) levels and T helper 2 (Th2) mediated responses in mice. L-Cystine dihydrochloride is promising for research of cystinuria and kidney stones
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 30925-07-6
- Formula: C6H14Cl2N2O4S2
- Molecular Weight:313.22
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Storage:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) L-Cystine dihydrochloride
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Customer Validation & Images
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ELISA
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Histological Imaging/Staining
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Cell Imaging/Staining
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WB
All Endogenous Metabolite Isoforms
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Biological Activity
L-Cystine dihydrochloride (83 µM or 200 µM, 72 h) effectively reverses the growth inhibition of both Sulfasalazine (HY-14655) and Erastin (HY-15763) in Huh6 cells[1]. L-Cystine dihydrochloride (0.8 mM, 4 h) induces Nrf2 protein expression elevation in a Keap1-dependent manner in HeLa cells[6].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Nine-week-old female BALB/c mice[2]
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Dosage:140, 280 and 560 mg/kg
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Administration:p.o. administration for 11 days
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Result:Increased IgG and IgM antibody production administrated with L-theanine (HY-15121) after DNP-dextran and DNP-KLH antigenic stimulation in mice.
Chemical Information
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CAS No. 30925-07-6
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Appearance Solid
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Molecular Weight 313.22
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Formula C6H14Cl2N2O4S2
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Color White to off-white
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Publications (5)
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Journal Impact Factor
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Most Recent
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Nature
2025 Jul;643(8070):192-200. PMID: 39695227 -
J Nanobiotechnology
Dual metabolic intervention nanoplatform co-delivering BAY-876 and L-cystine for Wilms tumor therapy via disulfidptosis-associated cytoskeletal collapse. [Abstract]2026 Jun 10. PMID: 42271349 -
Mater Today Bio
CD24-targeted cystine and glucose oxidase cascade catalytic nanosystem triggers disulfidptosis in neuroblastoma. [Abstract]2025 Nov 1:35:102496. PMID: 41281650 -
Mol Psychiatry
Correction of eIF4E overactivation rescues translatome imbalance and core ASD-like behaviors in valproic acid-induced offspring mice. [Abstract]2026 Mar 7. PMID: 41795048 -
Biochim Biophys Acta Mol Basis Dis
Mitigation of gestational diabetes-induced endothelial dysfunction through FGF21-NRF2 pathway activation involving L-Cystine. [Abstract]2024 Oct;1870(7):167329. PMID: 38960053
L-Cystine dihydrochloride purchased from MedChemExpress. Usage Cited in: Biochim Biophys Acta Mol Basis Dis. 2024 Oct;1870(7):167329. [Abstract]
HUVECs in the presence of 5.5 or 35 mM d-Glucose with or without Si-Scr, Si-FGF21, with or without L-Cystine (0.8 mM) for 96 h. The correlation between L-Cystine and FGF21 expression levels. The X-axis represents the concentration of FGF21 measured via ELISA assay (Log10 conversion). The Y-axis represents the intensity of L-Cystine mass spectrometry signal (Log10 conversion).
L-Cystine dihydrochloride purchased from MedChemExpress. Usage Cited in: Biochim Biophys Acta Mol Basis Dis. 2024 Oct;1870(7):167329. [Abstract]
HUVECs in the presence of 5.5 or 35 mM d-Glucose with or without Si-Scr, Si-FGF21, with or without L-Cystine (0.8 mM) for 96 h. DHE staining and TUNEL staining of HUVECs. Scale bar: 100 μm. The right bar chart explored the quantitative analysis the ROS level and the number of TUNEL positive HUVECs.
L-Cystine dihydrochloride purchased from MedChemExpress. Usage Cited in: Biochim Biophys Acta Mol Basis Dis. 2024 Oct;1870(7):167329. [Abstract]
HUVECs in the presence of 5.5 or 35 mM d-Glucose with or without Si-Scr, Si-FGF21, with or without L-Cystine (0.8 mM) for 96 h. A tube formation assay was performed to assess the angiogenesis ability of HUVECs after being treated as mentioned above. Scale bar: 100 μm. The tube length was quantified and is shown in right.
L-Cystine dihydrochloride purchased from MedChemExpress. Usage Cited in: Biochim Biophys Acta Mol Basis Dis. 2024 Oct;1870(7):167329. [Abstract]
HUVECs in the presence of 5.5 or 35 mM d-Glucose with or without Si-Scr, Si-FGF21, with or without L-Cystine (0.8 mM) for 96 h. A tube formation assay was performed to assess the angiogenesis ability of HUVECs after being treated as mentioned above. Scale bar: 100 μm. The tube length was quantified and is shown in right.
Solvent & Solubility
DMSO : 50 mg/mL (159.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (476 KB)
- English - EN (476 KB)
- Français - FR (476 KB)
- Deutsch - DE (476 KB)
- Norwegian - NO (476 KB)
- Español - ES (476 KB)
- Swedish - SV (476 KB)
- Italian - IT (476 KB)
- Korean - KR (476 KB)
- Portuguese - PT (476 KB)
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Handling Instructions (2659 KB)
References
[1]. Abdullah M, et al. Extracellular Concentration of L-Cystine Determines the Sensitivity to System xc- Inhibitors[J]. Biomol Ther (Seoul). 2022 Mar 1;30(2):184-190. [Content Brief]
[2]. Kurihara S, et al. Enhancement of antigen-specific immunoglobulin G production in mice by co-administration of L-cystine and L-theanine[J]. J Vet Med Sci. 2007 Dec;69(12):1263-70. [Content Brief]
[4]. Greth WE, et al. Cellular accumulation of L-cystine in rat kidney cortex in vivo[J]. J Clin Invest. 1973 Feb;52(2):454-62. [Content Brief]
[5]. Ohtsu I, et al. Uptake of L-cystine via an ABC transporter contributes defense of oxidative stress in the L-cystine export-dependent manner in Escherichia coli. PLoS One. 2015 Apr 2;10(3):e0120619. [Content Brief]
[6]. Dai W, et al. Fresh Medium or L-Cystine as an Effective Nrf2 Inducer for Cytoprotection in Cell Culture[J]. Cells. 2023 Jan 12;12(2):291. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1926 mL | 15.9632 mL | 31.9264 mL | 79.8161 mL |
| 5 mM | 0.6385 mL | 3.1926 mL | 6.3853 mL | 15.9632 mL | |
| 10 mM | 0.3193 mL | 1.5963 mL | 3.1926 mL | 7.9816 mL | |
| 15 mM | 0.2128 mL | 1.0642 mL | 2.1284 mL | 5.3211 mL | |
| 20 mM | 0.1596 mL | 0.7982 mL | 1.5963 mL | 3.9908 mL | |
| 25 mM | 0.1277 mL | 0.6385 mL | 1.2771 mL | 3.1926 mL | |
| 30 mM | 0.1064 mL | 0.5321 mL | 1.0642 mL | 2.6605 mL | |
| 40 mM | 0.0798 mL | 0.3991 mL | 0.7982 mL | 1.9954 mL | |
| 50 mM | 0.0639 mL | 0.3193 mL | 0.6385 mL | 1.5963 mL | |
| 60 mM | 0.0532 mL | 0.2661 mL | 0.5321 mL | 1.3303 mL | |
| 80 mM | 0.0399 mL | 0.1995 mL | 0.3991 mL | 0.9977 mL | |
| 100 mM | 0.0319 mL | 0.1596 mL | 0.3193 mL | 0.7982 mL |
L-Cystine dihydrochloride Related Classifications
HY-W009203 Related Classifications
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