L-Cystine dihydrochloride

Name: L-Cystine dihydrochloride
Brand: MedChemExpress
SKU: HY-W009203
Rating: 5.0 (4 reviews)

Cat. No.: HY-W009203 Purity: 98.0%: Data Sheet
COA

SDS
Handling Instructions
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L-Cystine dihydrochloride is the dihydrochloride salt form of L-Cystine (HY-N0394). L-Cystine dihydrochloride elevates Nrf2 protein expression and activates Nrf2 transcription factor. L-cystine dihydrochloride reduces ROS generation and protects against oxidant- or Doxorubicin (HY-15142A)-induced apoptosis. L-Cystine dihydrochloride combined with L-theanine (HY-15121) enhances the production of antigen-specific IgG by increasing glutathione (GSH) levels and T helper 2 (Th2) mediated responses in mice. L-Cystine dihydrochloride is promising for research of cystinuria and kidney stones

For research use only. We do not sell to patients.

Custom Peptide Synthesis

CAS No. : 30925-07-6

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	Get quote
5 g	Get quote

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Customer Review

Based on 4 publication(s) in Google Scholar

Other Forms of L-Cystine dihydrochloride:

L-Cystine In-stock
L-Cystine dihydrochloride (Standard) Get quote

4 Publications Citing Use of MCE L-Cystine dihydrochloride

ELISA

Histological Imaging/Staining

Cell Imaging/Staining

: L-Cystine dihydrochloride purchased from MedChemExpress. Usage Cited in: Biochim Biophys Acta Mol Basis Dis. 2024 Jul 1:167329. [Abstract]; HUVECs in the presence of 5.5 or 35 mM d-Glucose with or without Si-Scr, Si-FGF21, with or without L-Cystine (0.8 mM) for 96 h. The correlation between L-Cystine and FGF21 expression levels. The X-axis represents the concentration of FGF21 measured via ELISA assay (Log10 conversion). The Y-axis represents the intensity of L-Cystine mass spectrometry signal (Log10 conversion).

: L-Cystine dihydrochloride purchased from MedChemExpress. Usage Cited in: Biochim Biophys Acta Mol Basis Dis. 2024 Jul 1:167329. [Abstract]; HUVECs in the presence of 5.5 or 35 mM d-Glucose with or without Si-Scr, Si-FGF21, with or without L-Cystine (0.8 mM) for 96 h. DHE staining and TUNEL staining of HUVECs. Scale bar: 100 μm. The right bar chart explored the quantitative analysis the ROS level and the number of TUNEL positive HUVECs.

: L-Cystine dihydrochloride purchased from MedChemExpress. Usage Cited in: Biochim Biophys Acta Mol Basis Dis. 2024 Jul 1:167329. [Abstract]; HUVECs in the presence of 5.5 or 35 mM d-Glucose with or without Si-Scr, Si-FGF21, with or without L-Cystine (0.8 mM) for 96 h. A tube formation assay was performed to assess the angiogenesis ability of HUVECs after being treated as mentioned above. Scale bar: 100 μm. The tube length was quantified and is shown in right.

: L-Cystine dihydrochloride purchased from MedChemExpress. Usage Cited in: Biochim Biophys Acta Mol Basis Dis. 2024 Jul 1:167329. [Abstract]; HUVECs in the presence of 5.5 or 35 mM d-Glucose with or without Si-Scr, Si-FGF21, with or without L-Cystine (0.8 mM) for 96 h. A tube formation assay was performed to assess the angiogenesis ability of HUVECs after being treated as mentioned above. Scale bar: 100 μm. The tube length was quantified and is shown in right.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

L-Cystine dihydrochloride (83 µM or 200 µM, 72 h) effectively reverses the growth inhibition of both Sulfasalazine (HY-14655) and Erastin (HY-15763) in Huh6 cells^[1].
L-Cystine dihydrochloride (0.8 mM, 4 h) induces Nrf2 protein expression elevation in a Keap1-dependent manner in HeLa cells^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	Huh6 and Huh7
Concentration:	83-200 µM
Incubation Time:	24-72 h
Result:	Reversed the cell growth inhibition.

Western Blot Analysis^[6]

Cell Line:	HeLa
Concentration:	0.2-0.8 mM
Incubation Time:	4 h
Result:	Increased expression of Nrf2.

In Vivo

L-Cystine dihydrochloride (280 mg/kg, p.o., 11 days) administrated with L-theanine (HY-15121) enhances antigen-specific IgG production partly through augmentation of glutathione (GSH) levels and T helper 2 (Th2)-mediated responses in mice^[2].
L-Cystine dihydrochloride (200 mg/kg, p.o., 11 days) administrated with L-theanine (HY-15121) (80 mg/kg, p.o., 11 days) significantly decreases the serum IL-10/IFN-γ ratio 6 hours after DNP-KLH antigenic stimulation, but increases the serum IL-10/IFN-γ ratio 24 hours after DNP-KLH antigenic stimulation in mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Nine-week-old female BALB/c mice^[2]
Dosage:	140, 280 and 560 mg/kg
Administration:	p.o. administration for 11 days
Result:	Increased IgG and IgM antibody production administrated with L-theanine (HY-15121) after DNP-dextran and DNP-KLH antigenic stimulation in mice.

Molecular Weight

313.22

Formula

C₆H₁₄Cl₂N₂O₄S₂

CAS No.

30925-07-6

Appearance

Solid

Color

White to off-white

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (159.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1926 mL	15.9632 mL	31.9264 mL
5 mM	0.6385 mL	3.1926 mL	6.3853 mL
10 mM	0.3193 mL	1.5963 mL	3.1926 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.25 mg/mL (3.99 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.0%

Select Batch:

Data Sheet (278 KB) SDS (476 KB)

COA (247 KB) HNMR (116 KB)

Handling Instructions (2659 KB)

References

[1]. Abdullah M, et al. Extracellular Concentration of L-Cystine Determines the Sensitivity to System xc- Inhibitors[J]. Biomol Ther (Seoul). 2022 Mar 1;30(2):184-190. [Content Brief]

[2]. Kurihara S, et al. Enhancement of antigen-specific immunoglobulin G production in mice by co-administration of L-cystine and L-theanine[J]. J Vet Med Sci. 2007 Dec;69(12):1263-70. [Content Brief]

[3]. Dominique Bazin,et al. Cystinuria and cystinosis are usually related to L-cystine: is this really the case for cystinosis? A physicochemical investigation at micrometre and nanometre scale. Comptes Rendus. Chimie, Volume 25 (2022) no. S1, pp. 489-502.

[4]. Greth WE, et al. Cellular accumulation of L-cystine in rat kidney cortex in vivo[J]. J Clin Invest. 1973 Feb;52(2):454-62. [Content Brief]

[5]. Ohtsu I, et al. Uptake of L-cystine via an ABC transporter contributes defense of oxidative stress in the L-cystine export-dependent manner in Escherichia coli. PLoS One. 2015 Apr 2;10(3):e0120619. [Content Brief]

[6]. Dai W, et al. Fresh Medium or L-Cystine as an Effective Nrf2 Inducer for Cytoprotection in Cell Culture[J]. Cells. 2023 Jan 12;12(2):291. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.1926 mL	15.9632 mL	31.9264 mL	79.8161 mL
	5 mM	0.6385 mL	3.1926 mL	6.3853 mL	15.9632 mL
	10 mM	0.3193 mL	1.5963 mL	3.1926 mL	7.9816 mL
	15 mM	0.2128 mL	1.0642 mL	2.1284 mL	5.3211 mL
	20 mM	0.1596 mL	0.7982 mL	1.5963 mL	3.9908 mL
	25 mM	0.1277 mL	0.6385 mL	1.2771 mL	3.1926 mL
	30 mM	0.1064 mL	0.5321 mL	1.0642 mL	2.6605 mL
	40 mM	0.0798 mL	0.3991 mL	0.7982 mL	1.9954 mL
	50 mM	0.0639 mL	0.3193 mL	0.6385 mL	1.5963 mL
	60 mM	0.0532 mL	0.2661 mL	0.5321 mL	1.3303 mL
	80 mM	0.0399 mL	0.1995 mL	0.3991 mL	0.9977 mL
	100 mM	0.0319 mL	0.1596 mL	0.3193 mL	0.7982 mL