2. Apoptosis Metabolic Enzyme/Protease NF-κB Immunology/Inflammation
  3. Apoptosis Endogenous Metabolite Reactive Oxygen Species
  4. L-Theanine

L-Theanine  (Synonyms: L-Glutamic Acid γ-ethyl amide; Nγ-Ethyl-L-glutamine)

Cat. No.: HY-15121 Purity: 98.84%
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L-Theanine (L-Glutamic Acid γ-ethyl amide) is a non-protein amino acid contained in green tea leaves, which blocks the binding of L-glutamic acid to glutamate receptors in the brain, and with neuroprotective, anticancer and anti-oxidative activities. L-Theanine can pass through the blood–brain barrier and is orally active.

For research use only. We do not sell to patients.

L-Theanine Chemical Structure

L-Theanine Chemical Structure

CAS No. : 3081-61-6

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Solid + Solvent
10 mM * 1 mL in Water
ready for reconstitution
 USD 55 In-stock
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200 mg USD 70 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of L-Theanine:

L-Theanine Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE L-Theanine

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

L-Theanine (L-Glutamic Acid γ-ethyl amide) is a non-protein amino acid contained in green tea leaves, which blocks the binding of L-glutamic acid to glutamate receptors in the brain, and with neuroprotective, anticancer and anti-oxidative activities. L-Theanine can pass through the blood–brain barrier and is orally active[1][2][3].

In Vitro

L-Theanine (L-Glutamic Acid γ-ethyl amide) inhibits the incorporation of extracellular glutamine into neurons, resulting in the suppression of exocytotic release of glutamate[3].
L-Theanine (500 μM; 72 h) protects against excess Dopamine (HY-B0451)-induced neuronal death in presence of astrocytes and increases glutathione level in astrocytes[3].
L-Theanine (0-5 mM; 72 h) is involved in glutathione synthesis[3].
L-Theanine (0.1-5 mM; 24 h) dose-dependently inhibits the viability of melanoma but not normal epidermal melanocytes[4].
L-Theanine (1-5 mM; 24 h) arrests cell cycle at G0/G1 phase, suppresses cell migration, induces apoptosis in A375 cells[4].
L-Theanine (1-5 mM; 24 h) also affects the proliferation, migration, and apoptosis of B16–F10 melanoma cells[4].
L-Theanine shows protective effect on cadmium-induced apoptosis in PC12 cells by inhibiting the mitochondria-mediated pathway and decreased ROS production[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

L-Theanine Related Antibodies

Cell Proliferation Assay[4]

Cell Line: A375 and PIG1 cells
Concentration: 0.1, 0.5, 1, 2 and 5 mM
Incubation Time: 24 h
Result: Dose-dependently decreased the viability of A375, but not PIG1 cells.

Cell Cycle Analysis[4]

Cell Line: A375
Concentration: 1, 2 and 5 mM
Incubation Time: 24 h
Result: Led to a dose dependent accumulation of A375 cells in G0/G1 phase and prevented cells from entering the S phase.

Western Blot Analysis[4]

Cell Line: A375
Concentration: 1, 2 and 5 mM
Incubation Time: 24 h
Result: Remarkably reduced the expression of proliferating cell nuclear antigen (PCNA), decreased protein levels of cyclinD1, cyclinE1, and cyclin-dependent protein kinase (CDK2 and CDK4). Potentiated the expression of cyclin-dependent kinase inhibitor 1A (CDKN1A, p21).
Dose-dependently increased the levels of apoptosis-promoting proteins including BAX and cleaved-caspase3 and decreased the level of antiapoptotic protein BCL-2.
Concentration dependently reduced the protein levels of ICAM-1, VCAM-1, MMP9, and MMP2.
Dose-dependently increased the p53 expression.

Cell Migration Assay [4]

Cell Line: A375
Concentration: 1, 2 and 5 mM
Incubation Time: 24 h
Result: Apparently suppressed the migration.

Apoptosis Analysis[4]

Cell Line: A375
Concentration: 1, 2 and 5 mM
Incubation Time: 24 h
Result: Showed pro-apoptotic effect.

RT-PCR[4]

Cell Line: A375
Concentration: 1, 2 and 5 mM
Incubation Time: 24 h
Result: Markedly elevated the mRNA levels of Bmal1, Clock, Rora, and Rev-erbβ.
In Vivo

L-Theanine (4.0 mg/kg; p.o.; daily for 14 days) upregulates glutathione contents in striata of normal mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Healthy male ICR mice weighing 32–34 g[3]
Dosage: 4.0 mg/kg
Administration: Oral administration, daily for 14 days
Result: Significantly increased glutathione content in the striatum, but not in the midbrain.
Molecular Weight

174.20

Formula

C7H14N2O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC([C@@H](N)CCC(NCC)=O)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 150 mg/mL (861.08 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.7405 mL 28.7026 mL 57.4053 mL
5 mM 1.1481 mL 5.7405 mL 11.4811 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (574.05 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 98.84%

COA (243 KB) LCMS (259 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 5.7405 mL 28.7026 mL 57.4053 mL 143.5132 mL
5 mM 1.1481 mL 5.7405 mL 11.4811 mL 28.7026 mL
10 mM 0.5741 mL 2.8703 mL 5.7405 mL 14.3513 mL
15 mM 0.3827 mL 1.9135 mL 3.8270 mL 9.5675 mL
20 mM 0.2870 mL 1.4351 mL 2.8703 mL 7.1757 mL
25 mM 0.2296 mL 1.1481 mL 2.2962 mL 5.7405 mL
30 mM 0.1914 mL 0.9568 mL 1.9135 mL 4.7838 mL
40 mM 0.1435 mL 0.7176 mL 1.4351 mL 3.5878 mL
50 mM 0.1148 mL 0.5741 mL 1.1481 mL 2.8703 mL
60 mM 0.0957 mL 0.4784 mL 0.9568 mL 2.3919 mL
80 mM 0.0718 mL 0.3588 mL 0.7176 mL 1.7939 mL
100 mM 0.0574 mL 0.2870 mL 0.5741 mL 1.4351 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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L-Theanine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L-Theanine3081-61-6L-Glutamic Acid γ-ethyl amide Nγ-Ethyl-L-glutamineApoptosisEndogenous MetaboliteReactive Oxygen Speciesneuroprotectiveanticanceranti-oxidativeanti-stressglutathioneGSHInhibitorinhibitorinhibit

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