L-Theanine
Based on 5 publication(s) in Google Scholar
L-Theanine (L-Glutamic Acid γ-ethyl amide) is a non-protein amino acid contained in green tea leaves, which blocks the binding of L-glutamic acid to glutamate receptors in the brain, and with neuroprotective, anticancer and anti-oxidative activities. L-Theanine can pass through the blood–brain barrier and is orally active.
For research use only. We do not sell to patients.
- Purity: 99.64%
- CAS No.: 3081-61-6
- Formula: C7H14N2O3
- Molecular Weight:174.20
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) L-Theanine
MoreAll Endogenous Metabolite Isoforms
More
Biological Activity
L-Theanine (L-Glutamic Acid γ-ethyl amide) inhibits the incorporation of extracellular glutamine into neurons, resulting in the suppression of exocytotic release of glutamate[3].
L-Theanine (500 μM; 72 h) protects against excess Dopamine (HY-B0451)-induced neuronal death in presence of astrocytes and increases glutathione level in astrocytes[3].
L-Theanine (0-5 mM; 72 h) is involved in glutathione synthesis[3].
L-Theanine (0.1-5 mM; 24 h) dose-dependently inhibits the viability of melanoma but not normal epidermal melanocytes[4].
L-Theanine (1-5 mM; 24 h) arrests cell cycle at G0/G1 phase, suppresses cell migration, induces apoptosis in A375 cells[4].
L-Theanine (1-5 mM; 24 h) also affects the proliferation, migration, and apoptosis of B16–F10 melanoma cells[4].
L-Theanine shows protective effect on cadmium-induced apoptosis in PC12 cells by inhibiting the mitochondria-mediated pathway and decreased ROS production[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A375 and PIG1 cells
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Concentration:0.1, 0.5, 1, 2 and 5 mM
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Incubation Time:24 h
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Result:Dose-dependently decreased the viability of A375, but not PIG1 cells.
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Cell Line:A375
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Concentration:1, 2 and 5 mM
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Incubation Time:24 h
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Result:Led to a dose dependent accumulation of A375 cells in G0/G1 phase and prevented cells from entering the S phase.
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Cell Line:A375
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Concentration:1, 2 and 5 mM
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Incubation Time:24 h
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Result:Remarkably reduced the expression of proliferating cell nuclear antigen (PCNA), decreased protein levels of cyclinD1, cyclinE1, and cyclin-dependent protein kinase (CDK2 and CDK4). Potentiated the expression of cyclin-dependent kinase inhibitor 1A (CDKN1A, p21).
Dose-dependently increased the levels of apoptosis-promoting proteins including BAX and cleaved-caspase3 and decreased the level of antiapoptotic protein BCL-2.
Concentration dependently reduced the protein levels of ICAM-1, VCAM-1, MMP9, and MMP2.
Dose-dependently increased the p53 expression.
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Cell Line:A375
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Concentration:1, 2 and 5 mM
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Incubation Time:24 h
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Result:Apparently suppressed the migration.
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Cell Line:A375
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Concentration:1, 2 and 5 mM
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Incubation Time:24 h
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Result:Showed pro-apoptotic effect.
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Cell Line:A375
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Concentration:1, 2 and 5 mM
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Incubation Time:24 h
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Result:Markedly elevated the mRNA levels of Bmal1, Clock, Rora, and Rev-erbβ.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Healthy male ICR mice weighing 32–34 g[3]
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Dosage:4.0 mg/kg
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Administration:Oral administration, daily for 14 days
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Result:Significantly increased glutathione content in the striatum, but not in the midbrain.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 3081-61-6
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Appearance Solid
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Molecular Weight 174.20
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Formula C7H14N2O3
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Color White to off-white
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SMILES
OC([C@@H](N)CCC(NCC)=O)=O
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Synonyms
L-Glutamic Acid γ-ethyl amide; Nγ-Ethyl-L-glutamine
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (5)
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Journal Impact Factor
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Most Recent
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Mol Med
L-theanine alleviates myocardial ischemia/reperfusion injury by suppressing oxidative stress and apoptosis through activation of the JAK2/STAT3 pathway in mice. [Abstract]2024 Jun 28;30(1):98. PMID: 38943069 -
Phytother Res
l-Theanine Improves Learning and Memory Through Attenuation of NOX4-Mediated Ferroptosis in Hippocampal Neurons of Sleep-Deprived Mice. [Abstract]2025 Jul 19. PMID: 40682446 -
Poult Sci
L-Theanine attenuates oxidative damage induced by heat stress through the PI3K/AKT/Nrf2 signaling pathway in skeletal muscle cells. [Abstract]2025 Apr 4;104(6):105140. PMID: 40203618 -
Journal of Electroanalytical Chemistry 915 (2022) 116334
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Naunyn Schmiedebergs Arch Pharmacol
L-theanine prevents myocardial injury in sleep‑deprived mice by suppressing ferroptosis through SIRT1. [Abstract]2025 May 6. PMID: 40328913
Solvent & Solubility
H2O : 150 mg/mL (861.08 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (574.05 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (278 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Vuong QV, et al. L-Theanine: properties, synthesis and isolation from tea. J Sci Food Agric. 2011 Aug 30;91(11):1931-9. [Content Brief]
[2]. Kimura K, et al. L-Theanine reduces psychological and physiological stress responses. Biol Psychol. 2007 Jan;74(1):39-45. [Content Brief]
[3]. Takeshima M, et al. l-Theanine protects against excess dopamine-induced neurotoxicity in the presence of astrocytes. J Clin Biochem Nutr. 2016 Sep;59(2):93-99. [Content Brief]
[4]. Zhang R, et al. L-Theanine inhibits melanoma cell growth and migration via regulating expression of the clock gene BMAL1. Eur J Nutr. 2022 Mar;61(2):763-777. [Content Brief]
[5]. Ben P, et al. Protective Effect of L-Theanine on Cadmium-Induced Apoptosis in PC12 Cells by Inhibiting the Mitochondria-Mediated Pathway. Neurochem Res. 2015 Aug;40(8):1661-70. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 5.7405 mL | 28.7026 mL | 57.4053 mL | 143.5132 mL |
| 5 mM | 1.1481 mL | 5.7405 mL | 11.4811 mL | 28.7026 mL | |
| 10 mM | 0.5741 mL | 2.8703 mL | 5.7405 mL | 14.3513 mL | |
| 15 mM | 0.3827 mL | 1.9135 mL | 3.8270 mL | 9.5675 mL | |
| 20 mM | 0.2870 mL | 1.4351 mL | 2.8703 mL | 7.1757 mL | |
| 25 mM | 0.2296 mL | 1.1481 mL | 2.2962 mL | 5.7405 mL | |
| 30 mM | 0.1914 mL | 0.9568 mL | 1.9135 mL | 4.7838 mL | |
| 40 mM | 0.1435 mL | 0.7176 mL | 1.4351 mL | 3.5878 mL | |
| 50 mM | 0.1148 mL | 0.5741 mL | 1.1481 mL | 2.8703 mL | |
| 60 mM | 0.0957 mL | 0.4784 mL | 0.9568 mL | 2.3919 mL | |
| 80 mM | 0.0718 mL | 0.3588 mL | 0.7176 mL | 1.7939 mL | |
| 100 mM | 0.0574 mL | 0.2870 mL | 0.5741 mL | 1.4351 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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