L-Cystine
Based on 4 publication(s) in Google Scholar
L-Cystine is an orally active extracellular form of L-Cysteine (HY-Y0337), occurring in proteins of plants and animals. L-Cystine elevates Nrf2 protein expression and activates Nrf2 transcription factor. L-cystine reduces ROS generation and protects against oxidant- or Doxorubicin (HY-15142A)-induced apoptosis. L-Cystine combined with L-theanine (HY-15121) enhances the production of antigen-specific IgG by increasing glutathione (GSH) levels and T helper 2 (Th2) mediated responses in mice. L-Cystine is promising for research of cystinuria and kidney stones
For research use only. We do not sell to patients.
- Purity: 99.84%
- CAS No.: 56-89-3
- Formula: C6H12N2O4S2
- Molecular Weight:240.30
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) L-Cystine
More
Customer Validation & Images
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ELISA
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Histological Imaging/Staining
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Cell Imaging/Staining
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WB
All Endogenous Metabolite Isoforms
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Biological Activity
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Human Endogenous Metabolite |
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Cell Line
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Type | Value | Description | References |
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| KB 3-1 | IC50 |
3.12 mM
Compound: cystine
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Cytotoxicity against human KB-3-1 cells incubated for 72 hrs by MTT assay
Cytotoxicity against human KB-3-1 cells incubated for 72 hrs by MTT assay
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[PMID: 21657271] |
| KB-V1 | IC50 |
2.14 mM
Compound: cystine
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Cytotoxicity against drug-resistant human KBV1 cells expressing P-gp incubated for 72 hrs by MTT assay
Cytotoxicity against drug-resistant human KBV1 cells expressing P-gp incubated for 72 hrs by MTT assay
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[PMID: 21657271] |
L-Cystine (83 µM or 200 µM, 24 h or 72 h) effectively reverses the growth inhibition of both Sulfasalazine (HY-14655) and Erastin (HY-15763) in Huh6 cells[1]. L-Cystine ( 0.8 mM, 4 h) induces Nrf2 protein expression elevation in a Keap1-dependent manner in HeLa cells[6].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Huh6 and Huh7 cells
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Concentration:83 µM or 200 µM
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Incubation Time:72 h or 24 h
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Result:The degree of growth inhibition by Sulfasalazine (HY-14655) or Erastin (HY-15763) was greater in medium containing a physiological level of 83 μM L-cystine than in medium containing 200 μM L-cystine in Huh6 and Huh7 cells.
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Cell Line:HeLa cells
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Concentration:0.2 mM
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Incubation Time:4 h
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Result:Was sufficient for producing Nrf2 protein elevation at 0.2 mM in HeLa cells.
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Cell Line:HeLa cells
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Concentration:0.8 mM
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Incubation Time:4 h
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Result:Induced Nrf2 in a Keap1-dependent manner in HeLa cells.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Nine-week-old female BALB/c mice[2]
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Dosage:140, 280 and 560 mg/kg
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Administration:p.o. administration for 11 days
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Result:Significantly increased IgG and IgM antibody production administrated with L-theanine (HY-15121) after DNP-dextran and DNP-KLH antigenic stimulation in mice.
Chemical Information
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CAS No. 56-89-3
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Appearance Solid
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Molecular Weight 240.30
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Formula C6H12N2O4S2
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Color White to off-white
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SMILES
N[C@@H](CSSC[C@@H](C(O)=O)N)C(O)=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (4)
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Journal Impact Factor
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Most Recent
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Nature
2025 Jul;643(8070):192-200. PMID: 39695227 -
Mater Today Bio
CD24-targeted cystine and glucose oxidase cascade catalytic nanosystem triggers disulfidptosis in neuroblastoma. [Abstract]2025 Nov 1:35:102496. PMID: 41281650 -
Mol Psychiatry
Correction of eIF4E overactivation rescues translatome imbalance and core ASD-like behaviors in valproic acid-induced offspring mice. [Abstract]2026 Mar 7. PMID: 41795048 -
Biochim Biophys Acta Mol Basis Dis
Mitigation of gestational diabetes-induced endothelial dysfunction through FGF21-NRF2 pathway activation involving L-Cystine. [Abstract]2024 Jul 1:167329. PMID: 38960053
L-Cystine purchased from MedChemExpress. Usage Cited in: Biochim Biophys Acta Mol Basis Dis. 2024 Jul 1:167329. [Abstract]
HUVECs in the presence of 5.5 or 35 mM d-Glucose with or without Si-Scr, Si-FGF21, with or without L-Cystine (0.8 mM) for 96 h. The correlation between L-Cystine and FGF21 expression levels. The X-axis represents the concentration of FGF21 measured via ELISA assay (Log10 conversion). The Y-axis represents the intensity of L-Cystine mass spectrometry signal (Log10 conversion).
L-Cystine purchased from MedChemExpress. Usage Cited in: Biochim Biophys Acta Mol Basis Dis. 2024 Jul 1:167329. [Abstract]
HUVECs in the presence of 5.5 or 35 mM d-Glucose with or without Si-Scr, Si-FGF21, with or without L-Cystine (0.8 mM) for 96 h. DHE staining and TUNEL staining of HUVECs. Scale bar: 100 μm. The right bar chart explored the quantitative analysis the ROS level and the number of TUNEL positive HUVECs.
L-Cystine purchased from MedChemExpress. Usage Cited in: Biochim Biophys Acta Mol Basis Dis. 2024 Jul 1:167329. [Abstract]
HUVECs in the presence of 5.5 or 35 mM d-Glucose with or without Si-Scr, Si-FGF21, with or without L-Cystine (0.8 mM) for 96 h. A tube formation assay was performed to assess the angiogenesis ability of HUVECs after being treated as mentioned above. Scale bar: 100 μm. The tube length was quantified and is shown in right.
L-Cystine purchased from MedChemExpress. Usage Cited in: Biochim Biophys Acta Mol Basis Dis. 2024 Jul 1:167329. [Abstract]
HUVECs in the presence of 5.5 or 35 mM d-Glucose with or without Si-Scr, Si-FGF21, with or without L-Cystine (0.8 mM) for 96 h. A tube formation assay was performed to assess the angiogenesis ability of HUVECs after being treated as mentioned above. Scale bar: 100 μm. The tube length was quantified and is shown in right.
Solvent & Solubility
H2O : 33.33 mg/mL (138.70 mM; ultrasonic and adjust pH to 11 with 1 M NaOH)
0.1 M NaOH : 3.33 mg/mL (13.86 mM; ultrasonic and warming and adjust pH to 11 with NaOH and heat to 70°C)
0.1 M HCl : 2.5 mg/mL (10.40 mM; ultrasonic and adjust pH to 2 with HCl)
DMSO : < 1 mg/mL (insoluble or slightly soluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (278 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
[1]. Abdullah M, et al. Extracellular Concentration of L-Cystine Determines the Sensitivity to System xc- Inhibitors[J]. Biomol Ther (Seoul). 2022 Mar 1;30(2):184-190. [Content Brief]
[2]. Kurihara S, et al. Enhancement of antigen-specific immunoglobulin G production in mice by co-administration of L-cystine and L-theanine[J]. J Vet Med Sci. 2007 Dec;69(12):1263-70. [Content Brief]
[4]. Greth WE, et al. Cellular accumulation of L-cystine in rat kidney cortex in vivo[J]. J Clin Invest. 1973 Feb;52(2):454-62. [Content Brief]
[5]. Ohtsu I, et al. Uptake of L-cystine via an ABC transporter contributes defense of oxidative stress in the L-cystine export-dependent manner in Escherichia coli. PLoS One. 2015 Apr 2;10(3):e0120619. [Content Brief]
[6]. Dai W, et al. Fresh Medium or L-Cystine as an Effective Nrf2 Inducer for Cytoprotection in Cell Culture[J]. Cells. 2023 Jan 12;12(2):291. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| 0.1 M HCl / 0.1 M NaOH / H2O | 1 mM | 4.1615 mL | 20.8073 mL | 41.6146 mL | 104.0366 mL |
| 5 mM | 0.8323 mL | 4.1615 mL | 8.3229 mL | 20.8073 mL | |
| 10 mM | 0.4161 mL | 2.0807 mL | 4.1615 mL | 10.4037 mL | |
| H2O | 15 mM | 0.2774 mL | 1.3872 mL | 2.7743 mL | 6.9358 mL |
| 20 mM | 0.2081 mL | 1.0404 mL | 2.0807 mL | 5.2018 mL | |
| 25 mM | 0.1665 mL | 0.8323 mL | 1.6646 mL | 4.1615 mL | |
| 30 mM | 0.1387 mL | 0.6936 mL | 1.3872 mL | 3.4679 mL | |
| 40 mM | 0.1040 mL | 0.5202 mL | 1.0404 mL | 2.6009 mL | |
| 50 mM | 0.0832 mL | 0.4161 mL | 0.8323 mL | 2.0807 mL | |
| 60 mM | 0.0694 mL | 0.3468 mL | 0.6936 mL | 1.7339 mL | |
| 80 mM | 0.0520 mL | 0.2601 mL | 0.5202 mL | 1.3005 mL | |
| 100 mM | 0.0416 mL | 0.2081 mL | 0.4161 mL | 1.0404 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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