LIBX-A402
Based on 1 Customer Validation
LIBX-A402 is a selective, ATP-dependent inhibitor of ACSL4 (hACSL4, IC50=0.33 μM, Kd=3.3 μM) and an inhibitor of ferroptosis. LIBX-A402 targets the fatty acid-binding pocket of ACSL4 and prevents cells from undergoing ferroptosis. LIBX-A402 can be used in the research of cancer and Parkinson's disease.
For research use only. We do not sell to patients.
- Purity: 98.71%
- Formula: C18H15NO3
- Molecular Weight:293.32
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
Metabolism/Protease Compound Library
HY-L012
-
Anti-Cancer Compound Library
HY-L025
-
Ferroptosis Compound Library
HY-L051
-
Anti-Parkinson's Disease Compound Library
HY-L085
-
Neurodegenerative Disease-related Compound Library
HY-L086
-
Highly Selective Inhibitors Library
HY-L158
-
Cell Death Library
HY-L162
-
Bioactive Compound Library Max
HY-L181
-
MCE Bioactive Compound Library
HY-L001V
-
Bioactive Compound Library
HY-L001
-
High-Throughput Bioactive Compound Library
HY-L205
Biological Activity
|
ACSL4 |
LIBX-A402 potently protects MDA-MB-231 cells from RSL3-induced ferroptosis with an EC50 of 460 nM, and shows no cytotoxicity at concentrations up to 25 μM[1].
LIBX-A402 (2.5 μM; 4 h; co-incubated with Arachidonic acid (HY-109590) + iron for 48 h) significantly protects differentiated LUHMES cells against ferroptosis induced by Arachidonic acid + iron[1].
LIBX-A402 (2.5 μM; 4 h; co-incubated with Arachidonic acid+iron for 24 h) significantly reduces lipid peroxidation induced by Arachidonic acid+iron in differentiated LUHMES cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:Differentiated LUHMES cells
-
Concentration:2.5 μM (LIBX-A402); 20 μM (arachidonic acid); 20 μM (FeCl3-NTA)
-
Incubation Time:4 h (pretreatment); 48 h (incubation with arachidonic acid + iron)
-
Result:Significantly protected LUHMES cells from arachidonic acid + iron-induced ferroptosis, with cell viability comparable to ACSL4 siRNA-treated cells.
Chemical Information
-
Appearance Solid
-
Molecular Weight 293.32
-
Formula C18H15NO3
-
Color Light yellow to yellow
-
SMILES
O=C(C1=C(C2=CC(C)=CC=C2O1)NC(C)=O)C3=CC=CC=C3
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (340.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (278 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.4092 mL | 17.0462 mL | 34.0925 mL | 85.2311 mL |
| 5 mM | 0.6818 mL | 3.4092 mL | 6.8185 mL | 17.0462 mL | |
| 10 mM | 0.3409 mL | 1.7046 mL | 3.4092 mL | 8.5231 mL | |
| 15 mM | 0.2273 mL | 1.1364 mL | 2.2728 mL | 5.6821 mL | |
| 20 mM | 0.1705 mL | 0.8523 mL | 1.7046 mL | 4.2616 mL | |
| 25 mM | 0.1364 mL | 0.6818 mL | 1.3637 mL | 3.4092 mL | |
| 30 mM | 0.1136 mL | 0.5682 mL | 1.1364 mL | 2.8410 mL | |
| 40 mM | 0.0852 mL | 0.4262 mL | 0.8523 mL | 2.1308 mL | |
| 50 mM | 0.0682 mL | 0.3409 mL | 0.6818 mL | 1.7046 mL | |
| 60 mM | 0.0568 mL | 0.2841 mL | 0.5682 mL | 1.4205 mL | |
| 80 mM | 0.0426 mL | 0.2131 mL | 0.4262 mL | 1.0654 mL | |
| 100 mM | 0.0341 mL | 0.1705 mL | 0.3409 mL | 0.8523 mL |
LIBX-A402 Related Classifications
HY-175327 Related Classifications
Powered by Bioz