2. Metabolic Enzyme/Protease Apoptosis
  3. ACSL Family Ferroptosis
  4. LIBX-A402

LIBX-A402 is a selective, ATP-dependent inhibitor of ACSL4 (hACSL4, IC50=0.33 μM, Kd=3.3 μM) and an inhibitor of ferroptosis. LIBX-A402 targets the fatty acid-binding pocket of ACSL4 and prevents cells from undergoing ferroptosis. LIBX-A402 can be used in the research of cancer and Parkinson's disease.

For research use only. We do not sell to patients.

LIBX-A402

LIBX-A402 Chemical Structure

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

LIBX-A402 Related Antibodies

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ACSL4 ACSL5

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

LIBX-A402 is a selective, ATP-dependent inhibitor of ACSL4 (hACSL4, IC50=0.33 μM, Kd=3.3 μM) and an inhibitor of ferroptosis. LIBX-A402 targets the fatty acid-binding pocket of ACSL4 and prevents cells from undergoing ferroptosis. LIBX-A402 can be used in the research of cancer and Parkinson's disease[1].

IC50 & Target

ACSL4

 

In Vitro

LIBX-A402 potently protects MDA-MB-231 cells from RSL3-induced ferroptosis with an EC50 of 460 nM, and shows no cytotoxicity at concentrations up to 25 μM[1].
LIBX-A402 (2.5 μM; 4 h; co-incubated with Arachidonic acid (HY-109590) + iron for 48 h) significantly protects differentiated LUHMES cells against ferroptosis induced by Arachidonic acid + iron[1].
LIBX-A402 (2.5 μM; 4 h; co-incubated with Arachidonic acid+iron for 24 h) significantly reduces lipid peroxidation induced by Arachidonic acid+iron in differentiated LUHMES cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

LIBX-A402 Related Antibodies

Cell Viability Assay[1]

Cell Line: Differentiated LUHMES cells
Concentration: 2.5 μM (LIBX-A402); 20 μM (arachidonic acid); 20 μM (FeCl3-NTA)
Incubation Time: 4 h (pretreatment); 48 h (incubation with arachidonic acid + iron)
Result: Significantly protected LUHMES cells from arachidonic acid + iron-induced ferroptosis, with cell viability comparable to ACSL4 siRNA-treated cells.
Molecular Weight

293.32

Formula

C18H15NO3
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(C1=C(C2=CC(C)=CC=C2O1)NC(C)=O)C3=CC=CC=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (340.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4092 mL 17.0462 mL 34.0925 mL
5 mM 0.6818 mL 3.4092 mL 6.8185 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 5 mg/mL (17.05 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.4092 mL 17.0462 mL 34.0925 mL 85.2311 mL
5 mM 0.6818 mL 3.4092 mL 6.8185 mL 17.0462 mL
10 mM 0.3409 mL 1.7046 mL 3.4092 mL 8.5231 mL
15 mM 0.2273 mL 1.1364 mL 2.2728 mL 5.6821 mL
20 mM 0.1705 mL 0.8523 mL 1.7046 mL 4.2616 mL
25 mM 0.1364 mL 0.6818 mL 1.3637 mL 3.4092 mL
30 mM 0.1136 mL 0.5682 mL 1.1364 mL 2.8410 mL
40 mM 0.0852 mL 0.4262 mL 0.8523 mL 2.1308 mL
50 mM 0.0682 mL 0.3409 mL 0.6818 mL 1.7046 mL
60 mM 0.0568 mL 0.2841 mL 0.5682 mL 1.4205 mL
80 mM 0.0426 mL 0.2131 mL 0.4262 mL 1.0654 mL
100 mM 0.0341 mL 0.1705 mL 0.3409 mL 0.8523 mL
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LIBX-A402 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

LIBX-A402ACSL FamilyFerroptosisparkinson's diseaselipid peroxidationferroptotic cell deathfatty acid pocketLUHMES cellscancerferroptosisACSL3MDA-MB-231 cellsACSL4Inhibitorinhibitorinhibit

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