Maleimide
Based on 1 Customer Validation
Maleimide can be used for production of antibody-drug conjugate (ADC) which is used in cancer research. Maleimide also be leveraged for the preparation of fluorogenic probe, which is mainly used for the specific detection of thiol analytes.
For research use only. We do not sell to patients.
- Purity: 99.90%
- CAS No.: 541-59-3
- Formula: C4H3NO2
- Molecular Weight:97.07
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Maleimide binds rapidly and covalently to the sulfhydryl (-SH) group of cysteine residues, that can be used in the drug delivery system for improved cell uptake efficiency[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c nude mouse[3]
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Dosage:2 mg/kg M-GGLG-liposomes
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Administration:sc, twice in an interval of 2 weeks
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Result:Inhibited the tumor growth in a long term compared to the GGLG-liposomes did.
Chemical Information
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CAS No. 541-59-3
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Appearance Solid
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Molecular Weight 97.07
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Formula C4H3NO2
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Color White to light yellow
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SMILES
O=C(N1)C=CC1=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : ≥ 100 mg/mL (1030.18 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 50 mg/mL (515.09 mM; Need ultrasonic)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (25.75 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (25.75 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 60 mg/mL (618.11 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (642 KB)
- English - EN (642 KB)
- Français - FR (642 KB)
- Deutsch - DE (642 KB)
- Norwegian - NO (642 KB)
- Español - ES (642 KB)
- Swedish - SV (642 KB)
- Italian - IT (642 KB)
- Korean - KR (642 KB)
- Portuguese - PT (642 KB)
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Handling Instructions (2659 KB)
References
[1]. Renault K, et, al. Covalent Modification of Biomolecules through Maleimide-Based Labeling Strategies. Bioconjug Chem. 2018 Aug 15;29(8):2497-2513. [Content Brief]
[2]. Zhang S, et, al. A Maleimide-functionalized Tetraphenylethene for Measuring and Imaging Unfolded Proteins in Cells. Chem Asian J. 2019 Mar 15;14(6):904-909. [Content Brief]
[3]. Li T, et al., A novel application of maleimide for advanced drug delivery: in vitro and in vivo evaluation of maleimide-modified pH-sensitive liposomes. Int J Nanomedicine. 2013;8:3855-66. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O / DMSO | 1 mM | 10.3018 mL | 51.5092 mL | 103.0184 mL | 257.5461 mL |
| 5 mM | 2.0604 mL | 10.3018 mL | 20.6037 mL | 51.5092 mL | |
| 10 mM | 1.0302 mL | 5.1509 mL | 10.3018 mL | 25.7546 mL | |
| 15 mM | 0.6868 mL | 3.4339 mL | 6.8679 mL | 17.1697 mL | |
| 20 mM | 0.5151 mL | 2.5755 mL | 5.1509 mL | 12.8773 mL | |
| 25 mM | 0.4121 mL | 2.0604 mL | 4.1207 mL | 10.3018 mL | |
| 30 mM | 0.3434 mL | 1.7170 mL | 3.4339 mL | 8.5849 mL | |
| 40 mM | 0.2575 mL | 1.2877 mL | 2.5755 mL | 6.4387 mL | |
| 50 mM | 0.2060 mL | 1.0302 mL | 2.0604 mL | 5.1509 mL | |
| 60 mM | 0.1717 mL | 0.8585 mL | 1.7170 mL | 4.2924 mL | |
| 80 mM | 0.1288 mL | 0.6439 mL | 1.2877 mL | 3.2193 mL | |
| 100 mM | 0.1030 mL | 0.5151 mL | 1.0302 mL | 2.5755 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Maleimide Related Classifications
HY-W007324 Related Classifications
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