Matriptase-IN-2

Cat. No.: HY-W783268A Purity: 99.87%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Matriptase-IN-2 is a secondary amide inhibitor of matriptase derived from sulfonylated 3-amidinophenylalanine, with a K_i value of 5 nM. Matriptase acts as a novel initiator of cartilage matrix degradation in osteoarthritis, and Matriptase-IN-2 inhibits matriptase-mediated cartilage degradation. Matriptase-IN-2 is applicable for the research of osteoarthritis.

For research use only. We do not sell to patients.

Matriptase-IN-2 Chemical Structure

CAS No. : 1268874-09-4

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	Get quote
50 mg	Get quote

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Other Forms of Matriptase-IN-2:

Matriptase-IN-2 free base Get quote

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Purity & Documentation
References
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Description

In Vitro

Measurement of the release of (A) sulfated glycosaminoglycans (as a measure of proteoglycans) and (B) hydroxyproline (as a measure of collagen) over days 0 to 7 and cumulatively (days 0 to 14) evaluates the role of Matriptase-IN-2 (Compound 432) in cartilage destruction induced by osteoarthritis (OA)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

830.62

Formula

C₃₃H₃₅Cl₂F₆N₅O₇S

CAS No.

1268874-09-4

Appearance

Solid

Color

White to off-white

SMILES

OC(C(F)(F)F)=O.ClC1=C(C2=CC(S(N[C@H](C(N3CCC(CC3)CCN)=O)CC4=CC(C(N)=N)=CC=C4)(=O)=O)=CC=C2)C=CC(Cl)=C1.OC(C(F)(F)F)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (120.39 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.2039 mL	6.0196 mL	12.0392 mL
5 mM	0.2408 mL	1.2039 mL	2.4078 mL
10 mM	0.1204 mL	0.6020 mL	1.2039 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (3.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.87%

Select Batch:

Data Sheet (272 KB) SDS (252 KB)

COA (251 KB) HNMR (153 KB) RP-HPLC (170 KB) LCMS (237 KB)

Handling Instructions (2659 KB)

References

[1]. Andrew David Rowan, et al. Screening methods. WO2011023958. 2010-08-27.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.2039 mL	6.0196 mL	12.0392 mL	30.0980 mL
	5 mM	0.2408 mL	1.2039 mL	2.4078 mL	6.0196 mL
	10 mM	0.1204 mL	0.6020 mL	1.2039 mL	3.0098 mL
	15 mM	0.0803 mL	0.4013 mL	0.8026 mL	2.0065 mL
	20 mM	0.0602 mL	0.3010 mL	0.6020 mL	1.5049 mL
	25 mM	0.0482 mL	0.2408 mL	0.4816 mL	1.2039 mL
	30 mM	0.0401 mL	0.2007 mL	0.4013 mL	1.0033 mL
	40 mM	0.0301 mL	0.1505 mL	0.3010 mL	0.7524 mL
	50 mM	0.0241 mL	0.1204 mL	0.2408 mL	0.6020 mL
	60 mM	0.0201 mL	0.1003 mL	0.2007 mL	0.5016 mL
	80 mM	0.0150 mL	0.0752 mL	0.1505 mL	0.3762 mL
	100 mM	0.0120 mL	0.0602 mL	0.1204 mL	0.3010 mL

Matriptase-IN-2 Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Matriptase-IN-21268874-09-4Ser/Thr ProteaseSerine proteasesSerine endopeptidasesThreonine proteasescartilageosteoarthritisosteoarthritis patientsmatriptaseInhibitorinhibitorinhibit

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