Methylene Blue Staining Solution (1%)
Methylene Blue Staining Solution (1%) (Basic Blue 9) is a guanylyl cyclase (sGC), monoamine oxidase A (MAO-A) and NO synthase (NOS) inhibitor. Methylene Blue Staining Solution (1%) is a vasopressor and is often used as a dye in several medical procedures. Methylene Blue Staining Solution (1%) through the nitric oxide syntase/guanylate cyclase signalling pathway to reduce prepulse inhibition. Methylene Blue Staining Solution (1%) is a REDOX cycling compound and able to cross the blood-brain barrier. Methylene Blue Staining Solution (1%) is a Tau aggregation inhibitor. Methylene Blue Staining Solution (1%) is a photosensitizer and redox agent. Methylene Blue Staining Solution (1%) reduces cerebral edema, attenuated microglial activation and reduced neuroinflammation.
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
IC50 & Target
Guanylyl cyclase (sGC)[1].
Monoamine oxidase A (MAO-A)[1].
NO synthase (NOS)[1]
In Vitro
Methylene Blue Staining Solution (1%) (Basic Blue 9) (4.5 μM; BV2 microglia) alters the immune profile of LPS-activated BV2 microglia and decreases the level of CD14, IL-1β, TNF-α, and CCL2 mRNA[3].
1. Sample treatment:
1.1 For paraffin sections: dewax and rehydrate as usual.
1.2 For frozen sections: distilled water for 2 min.
1.3 For cultured cells: Fix with 4% paraformaldehyde. Wash with distilled water for 2 min. Replace with fresh distilled water and wash for another 2 min.
2. Methylene Blue Staining Solution (1%) staining
2.1 Stain with 0.1% Methylene Blue Staining Solution (1%) for 2 min.
2.2 Wash thoroughly with distilled water, observe and take photos.
2.3 Staining results:
Tissue or cells: blue
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Methylene Blue Staining Solution (1%) (Basic Blue 9) (20 and 40 mg/kg; p.o.; daily, for 6 months; CaMKIIα-tTA transactivator mice) preserves cognition in mice expressing full-length pro-aggregant human Tau[2].
Methylene Blue Staining Solution (1%) (Basic Blue 9) (2 mg/kg; i.v.; once, for 1 d; TBI-treated male BALB/c mice) reduces TBI-induced edema and neuroinflammation and reduces acute depression-like behavior[3].
Methylene Blue Staining Solution (1%) (Basic Blue 9) (2 mg/kg; i.v.; once, for 1 d; TBI-treated male BALB/c mice) reduces the percentage of inflammatory factor[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male NMRI mice[1]
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Dosage:50 and 100 mg/kg
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Administration:Intraperitoneal injection; once, for 25 minutes
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Result:Reduced the prepulse inhibition and reduced the increase in locomotor activity caused by phencyclidine (PCP).
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Animal Model:CaMKIIα-tTA transactivator mice[2]
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Dosage:20 and 40 mg/kg
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Administration:Oral administration; daily, for 6 months
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Result:Inhibited Tau aggregation in CaMKIIα-tTA transactivator mice.
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Animal Model:TBI-treated male BALB/c mice[3]
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Dosage:2 mg/kg
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Administration:Intravenous injection; once, for 1 day
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Result:Decreased the level of CD14, IL-1β, TNF-α, and CCL2 mRNA.
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Animal Model:TBI-treated male BALB/c mice[3]
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Dosage:2 mg/kg
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Administration:Intravenous injection; once, for 1 day
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Result:Reduced the percentage of myeloid (CD11b+/GR1+) cells, reduced IL-1β and enhanced IL-10 expression in microglia.
Chemical Information
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
[1]. Klamer D, et, al. Phencyclidine-induced behaviour in mice prevented by methylene blue. Basic Clin Pharmacol Toxicol. 2004 Feb;94(2):65-72. [Content Brief]
[2]. Hochgräfe K, et, al. Preventive methylene blue treatment preserves cognition in mice expressing full-length pro-aggregant human Tau. Acta Neuropathol Commun. 2015 May 10;3:25. [Content Brief]
[3]. Fenn AM, et, al. Methylene blue attenuates traumatic brain injury-associated neuroinflammation and acute depressive-like behavior in mice. J Neurotrauma. 2015 Jan 15;32(2):127-38. [Content Brief]
[4]. Kalaitzakis D, et al. Methylene Blue as a Photosensitizer and Redox Agent: Synthesis of 5-Hydroxy-1H-pyrrol-2(5H)-ones from Furans. Angew Chem Int Ed Engl. 2015 May 18;54(21):6283-7. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Methylene Blue Staining Solution (1%) Related Classifications
HY-DY2050 Related Classifications
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