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Art. -Nr.: HY-N21666

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Micranoic acid B

Micranoic acid B is a terpenoid compound. Micranoic acid B exhibits cytotoxicity against cancer cells and shows comparable activity against multidrug-resistant and parental cancer cell lines. Micranoic acid B can be used for research on lung cancer, prostate cancer, and nasopharyngeal epidermoid carcinoma.

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CAS. Nr.: 659738-09-7
Formel: C₂₂H₃₂O₃
Molecular Weight:344.50

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

Micranoic acid B Chemical Structure

CAS. Nr.: 659738-09-7

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
Chemical Information
Protokoll (3)
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	GI50	12.79 μM	Cytotoxicity against human lung carcinoma A549 cells assessed as 50% inhibition of cell growth incubated for 72 hrs by sulforhodamine B (SRB) colorimetric assay. Cytotoxicity against human lung carcinoma A549 cells assessed as 50% inhibition of cell growth incubated for 72 hrs by sulforhodamine B (SRB) colorimetric assay.	23883077
PC-3	GI50	11.83 μM	Cytotoxicity against human prostate carcinoma PC-3 cells assessed as 50% inhibition of cell growth incubated for 72 hrs by sulforhodamine B (SRB) colorimetric assay. Cytotoxicity against human prostate carcinoma PC-3 cells assessed as 50% inhibition of cell growth incubated for 72 hrs by sulforhodamine B (SRB) colorimetric assay.	23883077
KB	GI50	12.62 μM	Cytotoxicity against human epidermoid carcinoma of the nasopharynx KB cells assessed as 50% inhibition of cell growth incubated for 72 hrs by sulforhodamine B (SRB) colorimetric assay. Cytotoxicity against human epidermoid carcinoma of the nasopharynx KB cells assessed as 50% inhibition of cell growth incubated for 72 hrs by sulforhodamine B (SRB) colorimetric assay.	23883077

In Vitro

Micranoic acid B (compound 5) (72 h) exhibits moderate cytotoxicity against A549, PC-3, KB and KBvin human cancer cell lines with GI₅₀ values of 11.83-12.79 μM^[4].
Micranoic acid B (compound 93) is a norcycloartane triterpenoid isolated from Schisandra micrantha that features an octanortriterpenoid backbone lacking the entire C-17 side chain^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

Chemical Information

CAS. Nr. 659738-09-7
Molecular Weight 344.50
Formel C₂₂H₃₂O₃
SMILES

C(CC(O)=O)[C@]12[C@]3([C@]([C@@]4(C)[C@](C)(CC3)C(=O)CC4)(CC[C@H]1C(C)=C)[H])C2
Structure Classification
- Terpenoids
- Triterpenes
Initial Source
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Protokoll

All Cell Biology

Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.

Reinheit & Dokumentation

Handling Instructions (2659 KB)

Verweise

[1]. Rao G, et al. α-Glucosidase and Bacterial β-Glucuronidase Inhibitors from the Stems of Schisandra sphaerandra Staph. Pharmaceuticals (Basel). 2022 Mar 9;15(3):329.

[2]. Xiao WL, et al. Triterpenoids from the Schisandraceae family. Natural product reports. 2008 Oct;25(5):871-91. [Content Brief]

[3]. Zou J, et al. Cycloartane triterpenoids from the stems of Schisandra glaucescens and their bioactivity. Fitoterapia. 2012 Jul;83(5):926-31.

[4]. Lu Y, et al. Cytotoxic and potential anticancer constituents from the stems of Schisandra pubescens. Pharmaceutical biology. 2013 Sep;51(9):1204-7. [Content Brief]

[5]. Shu L, et al. Rapid identification of chemical compositions of three species of Schisandra chinensis by ultra-high-performance liquid chromatography quadrupole-orbitrap-mass spectrometry. Journal of separation science. 2023 Oct;46(20):e2300466.

[6]. He F, et al. Nortriterpene constituents from Schisandra sphenanthera. Tetrahedron. 2012 Jan 14;68(2):440-6.

[7]. Li RT, et al. Micranoic acids A and B: two new octanortriterpenoids from Schisandra micrantha. Chemical & pharmaceutical bulletin. 2003 Oct;51(10):1174-6.

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

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