2. GPCR/G Protein Neuronal Signaling
  3. mAChR
  4. (-)-Vesamicol

(-)-Vesamicol (AH5183) is a vesicular acetylcholine transporter inhibitor. (-)-Vesamicol reversibly and non-competitively inhibits the transport of acetylcholine into circulating synaptic vesicles and blocks the activity of vesicular acetylcholine transporters in medial amygdala neurons. (-)-Vesamicol is applicable to research related to central precocious puberty.

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(-)-Vesamicol

(-)-Vesamicol Chemical Structure

CAS No. : 112709-59-8

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

Other Forms of (-)-Vesamicol:

(-)-Vesamicol Related Antibodies

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1 Publications Citing Use of MCE (-)-Vesamicol

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  • Biological Activity

  • Purity & Documentation

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Description

(-)-Vesamicol (AH5183) is a vesicular acetylcholine transporter inhibitor. (-)-Vesamicol reversibly and non-competitively inhibits the transport of acetylcholine into circulating synaptic vesicles and blocks the activity of vesicular acetylcholine transporters in medial amygdala neurons. (-)-Vesamicol is applicable to research related to central precocious puberty[1][2].

In Vivo

(-)-Vesamicol (AH5183) (20 mmol; stereotactic injection) significantly delays precocious puberty onset induced by sexual content stimuli, reduces the serum LH/FSH ratio, and decreases kisspeptin expression in the ARC of female Sprague-Dawley rats[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Sprague-Dawley (female, postnatal day 21, central precocious puberty induced by combined odor and visual sexual content stimuli)[2]
Dosage: 20 mmol
Administration: stereotactic injection
Result: Delayed the timing of pubertal onset compared to the OVT-only group.
Suppressed the serum LH/FSH ratio compared to the OVT-only group .
Reduced the number of kisspeptin-positive cell bodies in the arcuate nucleus (ARC) compared to the OVT-only group.
Molecular Weight

259.39

Formula

C17H25NO
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1[C@H](N2CCC(C3=CC=CC=C3)CC2)CCCC1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 12.5 mg/mL (48.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.8552 mL 19.2760 mL 38.5520 mL
5 mM 0.7710 mL 3.8552 mL 7.7104 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: 5 mg/mL (19.28 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
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% 
+
%
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%
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.8552 mL 19.2760 mL 38.5520 mL 96.3800 mL
5 mM 0.7710 mL 3.8552 mL 7.7104 mL 19.2760 mL
10 mM 0.3855 mL 1.9276 mL 3.8552 mL 9.6380 mL
15 mM 0.2570 mL 1.2851 mL 2.5701 mL 6.4253 mL
20 mM 0.1928 mL 0.9638 mL 1.9276 mL 4.8190 mL
25 mM 0.1542 mL 0.7710 mL 1.5421 mL 3.8552 mL
30 mM 0.1285 mL 0.6425 mL 1.2851 mL 3.2127 mL
40 mM 0.0964 mL 0.4819 mL 0.9638 mL 2.4095 mL
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(-)-Vesamicol Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(-)-Vesamicol112709-59-8(-)-AH5183mAChRMuscarinic acetylcholine receptoracetylcholineGnRHarcuate nucleus kisspeptinfollicle-stimulating hormoneluteinizing hormoneminiature endplate currentevoked endplate currentvesicular acetylcholine transportersynaptic vesiclesmedial amygdala neuronsInhibitorinhibitorinhibit

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