2. GPCR/G Protein MAPK/ERK Pathway
  3. Ras
  4. ML-097

ML-097  (Synonyms: CID-2160985)

Cat. No.: HY-118208 Purity: ≥99.0%
COA Handling Instructions

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ML-097 (CID-2160985) is a pan Ras-related GTPases activator that can activate Rac1, cell division cycle 42, Ras, and Rab7.

For research use only. We do not sell to patients.

ML-097 Chemical Structure

ML-097 Chemical Structure

CAS No. : 743456-83-9

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 82 In-stock
Solution
10 mM * 1 mL in DMSO USD 82 In-stock
Solid
5 mg USD 75 In-stock
10 mg   Get quote  
50 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

ML-097 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE ML-097

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K-Ras H-Ras Ras

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

ML-097 (CID-2160985) is a pan Ras-related GTPases activator that can activate Rac1, cell division cycle 42, Ras, and Rab7[1].

In Vitro

ML-099 activates cell division cycle 42 activated mutant, cell division cycle 42 wild type, Ras protein wild type, GTP-binding protein (Rab7), Rac1 protein activated mutant, Rac1 protein wild type, and Ras protein activated mutant with EC50 values of 50.11 nM, 102.32 nM, 109.64 nM, 20.41 nM, 81.28 nM, 151.35 nM, and 93.32 nM, respectively[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ML-097 Related Antibodies
Molecular Weight

307.14

Formula

C14H11BrO3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

BrC1=C(C=CC=C1)COC2=C(C=CC=C2)C(O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (325.58 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2558 mL 16.2792 mL 32.5584 mL
5 mM 0.6512 mL 3.2558 mL 6.5117 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (8.14 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: ≥99.0%

COA (253 KB) LCMS (94 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.2558 mL 16.2792 mL 32.5584 mL 81.3961 mL
5 mM 0.6512 mL 3.2558 mL 6.5117 mL 16.2792 mL
10 mM 0.3256 mL 1.6279 mL 3.2558 mL 8.1396 mL
15 mM 0.2171 mL 1.0853 mL 2.1706 mL 5.4264 mL
20 mM 0.1628 mL 0.8140 mL 1.6279 mL 4.0698 mL
25 mM 0.1302 mL 0.6512 mL 1.3023 mL 3.2558 mL
30 mM 0.1085 mL 0.5426 mL 1.0853 mL 2.7132 mL
40 mM 0.0814 mL 0.4070 mL 0.8140 mL 2.0349 mL
50 mM 0.0651 mL 0.3256 mL 0.6512 mL 1.6279 mL
60 mM 0.0543 mL 0.2713 mL 0.5426 mL 1.3566 mL
80 mM 0.0407 mL 0.2035 mL 0.4070 mL 1.0175 mL
100 mM 0.0326 mL 0.1628 mL 0.3256 mL 0.8140 mL
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ML-097 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ML-097743456-83-9CID-2160985ML097ML 097CID2160985CID 2160985RasRac1Rab7cell division cycle 42Inhibitorinhibitorinhibit

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