Nemadipine-A
Based on 1 Customer Validation
Nemadipine-A is a specific inhibitor of the EGL-19 L-type Ca2+ channel. Nemadipine-A, a cell-permeable L-type calcium channel inhibitor, sensitizes TRAIL-resistant cancer cells to this ligand.
For research use only. We do not sell to patients.
- Purity: 98.87%
- CAS No.: 54280-71-6
- Formula: C19H18F5NO4
- Molecular Weight:419.34
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Nemadipine-A is a specific and highly effective inhibitor of EGL-19. Nemadipine-A (µM) is sufficient to abolish the enhanced level of paralysis observed in egl-19(ad695); crIs4 mutants[1].
Nemadipine-A strongly potentiates TRAIL-induced apoptosis in TRAIL-resistant lung cancer cells via the down-regulation of the anti-apoptotic protein survivin[2]. Nemadipine-A sensitizes H1299 lung cancer cells towards TRAIL-induced cytotoxicity[2]. Combination of Nemadipine-A (20 μM) with TRAIL (20 ng/mL) induces caspase-mediated apoptotic cell death[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:H1299 lung adenocarcinoma cells
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Concentration:0, 5, 10, 20, and 30 μM
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Incubation Time:8 hours
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Result:H1299 cells were resistant to the apoptotic effects of TRAIL, but the co-administration of Nemadipine-A and TRAIL in these cells resulted in notably increased cytotoxicity in a dose-dependent manner.
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Cell Line:H1299 cells
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Concentration:20 μM
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Incubation Time:8 hours
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Result:Co-treated with TRAIL and Nemadipine-A (T/N; 20 ng/mL and 20 μM respectively) induced cell death in H1299 cells.
Chemical Information
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CAS No. 54280-71-6
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Appearance Solid
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Molecular Weight 419.34
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Formula C19H18F5NO4
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Color White to light yellow
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SMILES
CC1=C(C(C(C(OCC)=O)=C(N1)C)C2=C(C(F)=C(C(F)=C2F)F)F)C(OCC)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 20 mg/mL (47.69 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Peter I Joyce, et al. The atypical calpains: evolutionary analyses and roles in Caenorhabditis elegans cellular. PLoS Genet. 2012;8(3):e1002602.degeneration [Content Brief]
[2]. Seong Ho Park, et al. Down-Regulation of Survivin by Nemadipine-A Sensitizes Cancer Cells to TRAIL-Induced Apoptosis. Biomol Ther (Seoul). 2013 Jan;21(1):29-34. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3847 mL | 11.9235 mL | 23.8470 mL | 59.6175 mL |
| 5 mM | 0.4769 mL | 2.3847 mL | 4.7694 mL | 11.9235 mL | |
| 10 mM | 0.2385 mL | 1.1923 mL | 2.3847 mL | 5.9617 mL | |
| 15 mM | 0.1590 mL | 0.7949 mL | 1.5898 mL | 3.9745 mL | |
| 20 mM | 0.1192 mL | 0.5962 mL | 1.1923 mL | 2.9809 mL | |
| 25 mM | 0.0954 mL | 0.4769 mL | 0.9539 mL | 2.3847 mL | |
| 30 mM | 0.0795 mL | 0.3974 mL | 0.7949 mL | 1.9872 mL | |
| 40 mM | 0.0596 mL | 0.2981 mL | 0.5962 mL | 1.4904 mL |
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