2. Metabolic Enzyme/Protease GPCR/G Protein
  3. Endogenous Metabolite Angiotensin Receptor
  4. Nitrosoglutathione

Nitrosoglutathione  (Synonyms: GSNO; RVC-588; S-Nitroso-L-glutathione)

Cat. No.: HY-D0845 Purity: 90.0%
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Nitrosoglutathione (GSNO), a exogenous NO donor and a substrate for rat alcohol dehydrogenase class III isoenzyme, inhibits cerebrovascular angiotensin II-dependent and -independent AT1 receptor responses.

For research use only. We do not sell to patients.

CAS No. : 57564-91-7

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
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Based on 9 publication(s) in Google Scholar

Other Forms of Nitrosoglutathione:

Nitrosoglutathione Related Antibodies

Top Publications Citing Use of Products

    Nitrosoglutathione purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Apr 27;16(1):3953.  [Abstract]

    Immunoblotting for EZH2 and H3K27me3 in cultured EA.hy926 cells exposed to GSNO (Nitrosoglutathione: 100 μM).

    Nitrosoglutathione purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Apr 27;16(1):3953.  [Abstract]

    Immunofluorescence followed by confocal imaging of EA.hy926 cells exposed to 1 h of GSNO (Nitrosoglutathione: 100 μM) and labeled for EZH2 (green) and F-actin (red). DAPI staining is shown in blue. White and yellow arrow head indicates EZH2 not bound and bound to F-actin respectively.

    Nitrosoglutathione purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Apr 27;16(1):3953.  [Abstract]

    EA.hy926 cells treated with GSNO (Nitrosoglutathione: 100 μM) for 0.5 h were subjected to co-immunoprecipitation using EZH2 antibody followed by immunoblotting for SUZ12.

    Nitrosoglutathione purchased from MedChemExpress. Usage Cited in: Small Sci. 2025 Jun 1;5(8):2500151.

    In PBS buffer (pH 5.5, 7.4, and 8.5), the cumulative NO generation of GSNO (Nitrosoglutathione: 50 μM) in the presence or absence of SeNPs (40 μg/mL) over 4 hours was measured.

    Nitrosoglutathione purchased from MedChemExpress. Usage Cited in: Small Sci. 2025 Jun 1;5(8):2500151.

    In PBS at pH 8.5, using 40 μg/mL SeNPs or Se@PDA NPs as catalysts, the accumulation of NO from GSNO (Nitrosoglutathione: 50 μM) over time was observed.

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    AT1 Receptor AT2 Receptor

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Nitrosoglutathione (GSNO), a exogenous NO donor and a substrate for rat alcohol dehydrogenase class III isoenzyme, inhibits cerebrovascular angiotensin II-dependent and -independent AT1 receptor responses[1][2][3][4].

    IC50 & Target

    AT1 Receptor

     

    Human Endogenous Metabolite

     

    In Vitro

    Nitrosoglutathione (GSNO, 250 μM) prevents 90% of the response to 0.1 μM 5-HT and 40% of the response to 1.0 μM 5-HT in rings treated with LY-83583, indicating an effect of GSNO that was independent of guanylate cyclase activity[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Nitrosoglutathione Related Antibodies
    In Vivo

    Nitrosoglutathione (GSNO, 8 mg/kg) significantly decreases systolic, diastolic, and mean arterial pressures in PE-induced rats from day 14 through day 20[3].
    Nitrosoglutathione (GSNO, 0.2 and 0.6 mg/kg) significantly inhibits superoxide production and suppressed NF-κB activation, iNOS induction, and 3-nitrotyrosine expression, but up-regulates endothelial NOS expression in the flap vessels[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male Lewis rats[4].
    Dosage: 0.2 and 0.6 mg/kg.
    Administration: Slow intravenous injection via the opposite femoral vein into each rat.
    Result: Animals treated with 0.2 mg of GSNO per kilogram before reperfusion had an intermediate survival rate (40.2 ± 4.9%). Although 0.6 mg/kg of GSNO showed a better rescuing effect than 150 mg/kg of NAC, there was no significant difference between the groups.
    Clinical Trial
    Molecular Weight

    336.32

    Formula

    C10H16N4O7S
    CAS No.
    Appearance

    Solid

    Color

    Pink to red

    SMILES

    OC(CNC([C@H](CSN=O)NC(CC[C@H](N)C(O)=O)=O)=O)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    H2O : 25 mg/mL (74.33 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.9734 mL 14.8668 mL 29.7336 mL
    5 mM 0.5947 mL 2.9734 mL 5.9467 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 90.0%

    SDS (480 KB)

    COA (245 KB) HNMR (256 KB) MS (177 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 2.9734 mL 14.8668 mL 29.7336 mL 74.3340 mL
    5 mM 0.5947 mL 2.9734 mL 5.9467 mL 14.8668 mL
    10 mM 0.2973 mL 1.4867 mL 2.9734 mL 7.4334 mL
    15 mM 0.1982 mL 0.9911 mL 1.9822 mL 4.9556 mL
    20 mM 0.1487 mL 0.7433 mL 1.4867 mL 3.7167 mL
    25 mM 0.1189 mL 0.5947 mL 1.1893 mL 2.9734 mL
    30 mM 0.0991 mL 0.4956 mL 0.9911 mL 2.4778 mL
    40 mM 0.0743 mL 0.3717 mL 0.7433 mL 1.8583 mL
    50 mM 0.0595 mL 0.2973 mL 0.5947 mL 1.4867 mL
    60 mM 0.0496 mL 0.2478 mL 0.4956 mL 1.2389 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Nitrosoglutathione Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Nitrosoglutathione57564-91-7GSNO RVC-588 S-Nitroso-L-glutathioneRVC588RVC 588RVC-588Endogenous MetaboliteAngiotensin ReceptorischemiaantioxidantI/RinjurysuperoxideNOInhibitorinhibitorinhibit

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