2-Aminoadenosine
Based on 1 Customer Validation
2-Aminoadenosine is a modified adenine base and adenosine analog that stabilizes RNA duplexes and enhances template-directed nucleotide condensation reactions. 2-Aminoadenosine forms three hydrogen bonds with uracil to increase the melting temperature of hybrid strands, thereby stabilizing uracil-containing RNA duplexes.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 2096-10-8
- Formula: C10H14N6O4
- Molecular Weight:282.26
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
2-Aminoadenosine-containing antisense oligonucleotide (3 hours at 30°C) depletion of U5 snRNP abolishes pre-mRNA splicing activity in HeLa cell nuclear extracts, and this activity is recoverable via complementation with extracts depleted of other spliceosomal snRNPs[1].
2-Aminoadenosine (0.1 M; 14 days at 0°C) increases U incorporation efficiency to 30% and enhances G incorporation efficiency in template-directed non-enzymatic oligonucleotide synthesis when substituted into a poly(CA) template[2].
2-Aminoadenosine (0.025-0.1 M; 14 days at 0°C) maintains high G incorporation efficiency (91% for poly(C2.9U1) template, 64% for poly(C1U1) template) at reduced substrate concentrations, whereas adenosine-based substrates show drastically reduced G incorporation efficiency at lower concentrations[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2096-10-8
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Appearance Solid
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Molecular Weight 282.26
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Formula C10H14N6O4
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Color White to off-white
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SMILES
OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(N=C(N)N=C3N)=C3N=C2)O1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (354.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.86 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.86 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Lamm GM, et al. Antisense probes containing 2-aminoadenosine allow efficient depletion of U5 snRNP from HeLa splicing extracts. Nucleic Acids Res. 1991;19(12):3193-3198. [Content Brief]
[2]. Grzeskowiak K, et al. Template-directed synthesis with 2-aminoadenosine. J Mol Evol. 1984;21(1):81-83. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.5428 mL | 17.7142 mL | 35.4283 mL | 88.5708 mL |
| 5 mM | 0.7086 mL | 3.5428 mL | 7.0857 mL | 17.7142 mL | |
| 10 mM | 0.3543 mL | 1.7714 mL | 3.5428 mL | 8.8571 mL | |
| 15 mM | 0.2362 mL | 1.1809 mL | 2.3619 mL | 5.9047 mL | |
| 20 mM | 0.1771 mL | 0.8857 mL | 1.7714 mL | 4.4285 mL | |
| 25 mM | 0.1417 mL | 0.7086 mL | 1.4171 mL | 3.5428 mL | |
| 30 mM | 0.1181 mL | 0.5905 mL | 1.1809 mL | 2.9524 mL | |
| 40 mM | 0.0886 mL | 0.4429 mL | 0.8857 mL | 2.2143 mL | |
| 50 mM | 0.0709 mL | 0.3543 mL | 0.7086 mL | 1.7714 mL | |
| 60 mM | 0.0590 mL | 0.2952 mL | 0.5905 mL | 1.4762 mL | |
| 80 mM | 0.0443 mL | 0.2214 mL | 0.4429 mL | 1.1071 mL | |
| 100 mM | 0.0354 mL | 0.1771 mL | 0.3543 mL | 0.8857 mL |
2-Aminoadenosine Related Classifications
HY-W011548 Related Classifications
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