STM3006
Based on 2 publication(s) in Google Scholar
STM3006 is a highly potent, selective, and orally active inhibitor of METTL3 (IC50: 5 nM). STM3006 can reduce the m6A level, promote the formation of dsRNA, trigger a cell-intrinsic interferon response, and enhance the killing effect of T cells on tumors. STM3006 has anti-tumor activity, and its combination with anti-PD-1 immunotherapy yields better results.
For research use only. We do not sell to patients.
- Purity : 99.79%
- CAS No.: 2499664-52-5
- Formula: C25H27BrN8
- Molecular Weight:519.44
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) STM3006
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Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Caov-3 cell line | IC50 |
182 nM
Compound: 6e
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Antiproliferative activity against human Caov-3 cell line assessed as inhibition of cell proliferation and measured after 7 days by CellTiter-Glo assay
Antiproliferative activity against human Caov-3 cell line assessed as inhibition of cell proliferation and measured after 7 days by CellTiter-Glo assay
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[PMID: 36692498] |
| Huh-7 | GI50 |
0.18 μM
Compound: 9; STM3006
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Growth inhibition of human Huh-7 cells
Growth inhibition of human Huh-7 cells
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[PMID: 38805939] |
| MOLM-13 | IC50 |
338 μM
Compound: 6e
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Antiproliferative activity against human MOLM-13 cells assessed as inhibition of cell proliferation and measured after 7 days by CellTiter-Glo assay
Antiproliferative activity against human MOLM-13 cells assessed as inhibition of cell proliferation and measured after 7 days by CellTiter-Glo assay
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[PMID: 36692498] |
In Vitro
STM3006 (0.1-0.5 μM; 30 h) can promote interferon activation and the expression of related genes in CaOV3[1].
STM3006 (0.3-3 μM; 48 h) enhances the killing effect of T cells on tumor cells in the co-culture experiment of mouse B16-ovalbumin tumor cells and OT-I T cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:CaOV3 cells
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Concentration:0.1 and 0.5 μmol/L
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Incubation Time:30 h
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Result:Enhanced the expression levels of MDA-5, IFIT1, OAS2, and ISG15.
Chemical Information
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CAS No. 2499664-52-5
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Appearance Solid
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Molecular Weight 519.44
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Formula C25H27BrN8
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Color Light yellow to brown
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SMILES
BrC1=CC(C2=CN(CC3=CN4C=C(CN5CCC(C)(CC5)C)C=CC4=N3)N=N2)=C6C=NNC6=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (2)
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Journal Impact Factor
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Most Recent
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bioRxiv
2026 Mar 18:2026.03.18.712554. PMID: 41890003 -
Solvent & Solubility
In Vitro:
DMSO : ≥ 100 mg/mL (192.52 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.81 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.81 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
[1]. Andrew A Guirguis, et al. Inhibition of METTL3 Results in a Cell-Intrinsic Interferon Response That Enhances Antitumor Immunity.Cancer Discov. 2023 Oct 5;13(10):2228-2247. [Content Brief]
[2]. Brichkina A, et al. Methyltransferase-like 3 (METTL3) inhibition potentiates anti-tumor immunity: a novel strategy for improving anti-PD1 therapy. Signal Transduct Target Ther. 2023 Dec 11;8(1):448. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9252 mL | 9.6258 mL | 19.2515 mL | 48.1288 mL |
| 5 mM | 0.3850 mL | 1.9252 mL | 3.8503 mL | 9.6258 mL | |
| 10 mM | 0.1925 mL | 0.9626 mL | 1.9252 mL | 4.8129 mL | |
| 15 mM | 0.1283 mL | 0.6417 mL | 1.2834 mL | 3.2086 mL | |
| 20 mM | 0.0963 mL | 0.4813 mL | 0.9626 mL | 2.4064 mL | |
| 25 mM | 0.0770 mL | 0.3850 mL | 0.7701 mL | 1.9252 mL | |
| 30 mM | 0.0642 mL | 0.3209 mL | 0.6417 mL | 1.6043 mL | |
| 40 mM | 0.0481 mL | 0.2406 mL | 0.4813 mL | 1.2032 mL | |
| 50 mM | 0.0385 mL | 0.1925 mL | 0.3850 mL | 0.9626 mL | |
| 60 mM | 0.0321 mL | 0.1604 mL | 0.3209 mL | 0.8021 mL | |
| 80 mM | 0.0241 mL | 0.1203 mL | 0.2406 mL | 0.6016 mL | |
| 100 mM | 0.0193 mL | 0.0963 mL | 0.1925 mL | 0.4813 mL |
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