TPP-9476
TPP-9476 (BAY-943 antibody) is an anti-human IL3RA (CD123) monoclonal antibody with human IL3RA Kd of 11 nM and cynomolgus monkey IL3RA Kd of 16 nM. TPP-9476 binds specifically to human and cynomolgus monkey IL3RA, undergoes target-dependent internalization into lysosomes of IL3RA-positive cells.TPP-9476 exerts antiproliferative effects in IL3RA-expressing acute myeloid leukemia and classical Hodgkin lymphoma cells, reduces tumor burden, improves survival, and induces complete tumor remission in relevant xenograft mouse models.
For research use only. We do not sell to patients.
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Human
TPP-9476 (IL3RA-targeting antibody) (titrated log M values) binds with high affinity to recombinant human and cynomolgus monkey IL3RA protein, and shows specific, dose-dependent binding to IL3RA-positive human acute myeloid leukemia cell lines MV-4-11, MOLM-13, and KG-1, with no binding to murine IL3RA[1].
TPP-9476 (IL3RA-targeting antibody) (10-20 μg/mL; 0-6 h) undergoes specific, time-dependent internalization into IL3RA-positive MOLM-13 and MV-4-11 acute myeloid leukemia cells, and colocalizes with lysosomes in MOLM-13 cells but not IL3RA-negative HBL-1 cells[1].
TPP-9476 (IL3RA-targeting antibody) (up to 3.00×10-7 M; 72 h) alone has no antiproliferative activity in IL3RA-positive human acute myeloid leukemia and Hodgkin lymphoma cell lines, even at concentrations up to 3.00×10-7 M after 72 h incubation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MV-4-11, MOLM-13, HDLM-2, THP-1, NCI-H292
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Concentration:Up to 3.00 × 10-7 M
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Incubation Time:72 h
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Result:Showed no antiproliferative activity (IC50 > 3.00 × 10-7 M) in IL3RA-positive MV-4-11, MOLM-13, HDLM-2, THP-1, and IL3RA-low NCI-H292 cell lines.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
IL-3Ra/CD123
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
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Product Image
ELISA, FACS, Functional assay
Chemical Information
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SMILES
[TPP-9476]
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Synonyms
BAY-943 antibody
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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