Cat. No.: HY-144222

NSC 828467

NSC 828467 exhibited significant in vitro anticancer activity,and it is one of the top-five CA IX inhibitors with an IC₅₀ value of 27.2nM.

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CAS No.: 3033384-72-1
Formula: C₂₁H₁₉N₉O₂S
Molecular Weight:461.50

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Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (268 KB)

NSC 828467 Chemical Structure

CAS No.: 3033384-72-1

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
Chemical Information
Purity & Documentation
References
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Biological Activity

Description

NSC 828467 exhibited significant in vitro anticancer activity,and it is one of the top-five CA IX inhibitors with an IC₅₀ value of 27.2nM.

IC₅₀ & Target

HCA IX

27.2 nM (IC₅₀)

All Carbonic Anhydrase Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
786-0	GI₅₀	1.06 μM Compound: 5k	Anticancer activity against human 786-0 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human 786-0 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
A498	GI₅₀	1.61 μM Compound: 5k	Anticancer activity against human A498 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human A498 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
A549	GI₅₀	2.29 μM Compound: 5k	Anticancer activity against human A549 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human A549 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
ACHN	GI₅₀	1.12 μM Compound: 5k	Anticancer activity against human ACHN cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human ACHN cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
BT-549	GI₅₀	0.89 μM Compound: 5k	Anticancer activity against human BT-549 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human BT-549 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
CAKI-1	GI₅₀	0.61 μM Compound: 5k	Anticancer activity against human CAKI-1 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human CAKI-1 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
CCRF-CEM	GI₅₀	0.31 μM Compound: 5k	Anticancer activity against human CCRF-CEM cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human CCRF-CEM cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
COLO 205	GI₅₀	0.41 μM Compound: 5k	Anticancer activity against human COLO 205 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human COLO 205 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
DU-145	GI₅₀	0.85 μM Compound: 5k	Anticancer activity against human DU-145 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human DU-145 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
EKVX	GI₅₀	0.71 μM Compound: 5k	Anticancer activity against human EKVX cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human EKVX cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
HCC 2998	GI₅₀	0.65 μM Compound: 5k	Anticancer activity against human HCC 2998 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human HCC 2998 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
HCC 2998	IC₅₀	1.93 μM Compound: 5k	Cytotoxicity against human HCC 2998 cells assessed as inhibition of cell viability incubated for 72 hrs under hypoxia condition by MTT assay Cytotoxicity against human HCC 2998 cells assessed as inhibition of cell viability incubated for 72 hrs under hypoxia condition by MTT assay	[PMID: 35182815]
HCT-116	GI₅₀	0.39 μM Compound: 5k	Anticancer activity against human HCT-116 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human HCT-116 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
HCT-15	GI₅₀	1.45 μM Compound: 5k	Anticancer activity against human HCT-15 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human HCT-15 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
HEK293	IC₅₀	45.64 μM Compound: 5k	Cytotoxicity against human HEK293 cells assessed as inhibition of cell viability by MTT assay Cytotoxicity against human HEK293 cells assessed as inhibition of cell viability by MTT assay	[PMID: 35182815]
HL-60(TB)	GI₅₀	0.63 μM Compound: 5k	Anticancer activity against human HL-60(TB) cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human HL-60(TB) cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
HOP-62	GI₅₀	0.55 μM Compound: 5k	Anticancer activity against human HOP-62 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human HOP-62 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
HOP-92	GI₅₀	0.8 μM Compound: 5k	Anticancer activity against human HOP-92 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human HOP-92 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
Hs-578T	GI₅₀	1.88 μM Compound: 5k	Anticancer activity against human Hs-578T cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human Hs-578T cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
HT-29	GI₅₀	2.15 μM Compound: 5k	Anticancer activity against human HT-29 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human HT-29 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
IGROV-1	GI₅₀	0.79 μM Compound: 5k	Anticancer activity against human IGROV-1 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human IGROV-1 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
K562	GI₅₀	0.4 μM Compound: 5k	Anticancer activity against human K562 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human K562 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
KM12	GI₅₀	2.14 μM Compound: 5k	Anticancer activity against human KM12 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human KM12 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
M14	GI₅₀	0.85 μM Compound: 5k	Anticancer activity against human M14 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human M14 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
Malme-3M	GI₅₀	1 μM Compound: 5k	Anticancer activity against human Malme-3M cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human Malme-3M cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
MCF7	GI₅₀	1.1 μM Compound: 5k	Anticancer activity against human MCF7 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human MCF7 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
MDA-MB-231	GI₅₀	0.5 μM Compound: 5k	Anticancer activity against human MDA-MB-231 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human MDA-MB-231 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
MDA-MB-435	GI₅₀	0.91 μM Compound: 5k	Anticancer activity against human MDA-MB-435 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human MDA-MB-435 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
MDA-MB-468	GI₅₀	0.31 μM Compound: 5k	Anticancer activity against human MDA-MB-468 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human MDA-MB-468 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
MOLT-4	GI₅₀	0.43 μM Compound: 5k	Anticancer activity against human MOLT-4 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human MOLT-4 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
NCI/ADR-RES	GI₅₀	2.75 μM Compound: 5k	Anticancer activity against human NCI/ADR-RES cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human NCI/ADR-RES cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
NCI-H226	GI₅₀	1.07 μM Compound: 5k	Anticancer activity against human NCI-H226 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human NCI-H226 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
NCI-H23	GI₅₀	1.21 μM Compound: 5k	Anticancer activity against human NCI-H23 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human NCI-H23 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
NCI-H322M	GI₅₀	0.4 μM Compound: 5k	Anticancer activity against human NCI-H322M cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human NCI-H322M cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
NCI-H460	GI₅₀	2.41 μM Compound: 5k	Anticancer activity against human NCI-H460 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human NCI-H460 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
NCI-H522	GI₅₀	0.45 μM Compound: 5k	Anticancer activity against human NCI-H522 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human NCI-H522 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
OVCAR-3	GI₅₀	0.46 μM Compound: 5k	Anticancer activity against human OVCAR-3 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human OVCAR-3 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
OVCAR-4	GI₅₀	0.44 μM Compound: 5k	Anticancer activity against human OVCAR-4 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human OVCAR-4 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
OVCAR-5	GI₅₀	1.6 μM Compound: 5k	Anticancer activity against human OVCAR-5 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human OVCAR-5 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
OVCAR-8	GI₅₀	0.44 μM Compound: 5k	Anticancer activity against human OVCAR-8 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human OVCAR-8 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
PC-3	GI₅₀	0.69 μM Compound: 5k	Anticancer activity against human PC-3 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human PC-3 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
RPMI-8226	GI₅₀	2.11 μM Compound: 5k	Anticancer activity against human RPMI-8226 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human RPMI-8226 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
RXF 393	GI₅₀	1.07 μM Compound: 5k	Anticancer activity against human RXF 393 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human RXF 393 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
RXF 393	IC₅₀	3.11 μM Compound: 5k	Cytotoxicity against human RXF 393 cells assessed as inhibition of cell viability incubated for 72 hrs under hypoxia condition by MTT assay Cytotoxicity against human RXF 393 cells assessed as inhibition of cell viability incubated for 72 hrs under hypoxia condition by MTT assay	[PMID: 35182815]
SF-268	GI₅₀	0.46 μM Compound: 5k	Anticancer activity against human SF-268 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human SF-268 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
SF-295	GI₅₀	1.4 μM Compound: 5k	Anticancer activity against human SF-295 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human SF-295 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
SF-539	GI₅₀	0.68 μM Compound: 5k	Anticancer activity against human SF-539 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human SF-539 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
SK-MEL-2	GI₅₀	1.5 μM Compound: 5k	Anticancer activity against human SK-MEL-2 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human SK-MEL-2 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
SK-MEL-28	GI₅₀	0.75 μM Compound: 5k	Anticancer activity against human SK-MEL-28 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human SK-MEL-28 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
SK-MEL-5	GI₅₀	0.78 μM Compound: 5k	Anticancer activity against human SK-MEL-5 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human SK-MEL-5 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
SK-MEL-5	IC₅₀	8.33 μM Compound: 5k	Cytotoxicity against human SK-MEL-5 cells assessed as inhibition of cell viability incubated for 72 hrs under hypoxia condition by MTT assay Cytotoxicity against human SK-MEL-5 cells assessed as inhibition of cell viability incubated for 72 hrs under hypoxia condition by MTT assay	[PMID: 35182815]
SK-OV-3	GI₅₀	0.84 μM Compound: 5k	Anticancer activity against human SK-OV-3 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human SK-OV-3 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
SN12C	GI₅₀	1.01 μM Compound: 5k	Anticancer activity against human SN12C cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human SN12C cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
SNB-19	GI₅₀	0.85 μM Compound: 5k	Anticancer activity against human SNB-19 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human SNB-19 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
SNB-75	GI₅₀	1.32 μM Compound: 5k	Anticancer activity against human SNB-75 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human SNB-75 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
SR	GI₅₀	0.33 μM Compound: 5k	Anticancer activity against human SR cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human SR cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
SW-620	GI₅₀	0.71 μM Compound: 5k	Anticancer activity against human SW-620 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human SW-620 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
T47D	GI₅₀	0.47 μM Compound: 5k	Anticancer activity against human T47D cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human T47D cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
TK-10	GI₅₀	1.19 μM Compound: 5k	Anticancer activity against human TK-10 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human TK-10 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
U-251	GI₅₀	0.51 μM Compound: 5k	Anticancer activity against human U-251 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human U-251 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
UACC-257	GI₅₀	2.14 μM Compound: 5k	Anticancer activity against human UACC-257 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human UACC-257 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
UACC-62	GI₅₀	0.57 μM Compound: 5k	Anticancer activity against human UACC-62 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human UACC-62 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]
UO-31	GI₅₀	1.5 μM Compound: 5k	Anticancer activity against human UO-31 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay Anticancer activity against human UO-31 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay	[PMID: 35182815]

Chemical Information

CAS No. 3033384-72-1
Molecular Weight 461.50
Formula C₂₁H₁₉N₉O₂S
SMILES

NS(=O)(C1=CC=C(C=C1)NC2=NC(N/N=C/C3=NC=CC=C3)=NC(NC4=CC=CC=C4)=N2)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (268 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Tawfik HO, et al. Discovery of new carbonic anhydrase IX inhibitors as anticancer agents by toning the hydrophobic and hydrophilic rims of the active site to encounter the dual-tail approach. Eur J Med Chem. 2022 Feb 12;232:114190. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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