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OSMI-3 

Cat. No.: HY-135785
Handling Instructions

OSMI-3 (Compound 2b) is a potent, long-lasting, and cell-permeable O-linked N-acetylglucosamine transferase (OGT) inhibitor. Cells contain a large nuclear pool of partially spliced OGT transcript, and OSMI-3 increases detained intron splicing in cells.

For research use only. We do not sell to patients.

OSMI-3 Chemical Structure

OSMI-3 Chemical Structure

CAS No. : 2260791-13-5

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Description

OSMI-3 (Compound 2b) is a potent, long-lasting, and cell-permeable O-linked N-acetylglucosamine transferase (OGT) inhibitor. Cells contain a large nuclear pool of partially spliced OGT transcript, and OSMI-3 increases detained intron splicing in cells[1].

IC50 & Target

O-linked N-acetylglucosamine transferase (OGT)[1]

In Vitro

OSMI-3 (Compound 2b; 20-50 μM; 4-24 hours; HCT116 cells) treatment significantly reduces O-GlcNAc levels, and has more sustained cellular effects than OSMI-2[1].
A decrease in HCF-1 cleavage products and the appearance of uncleaved HCF-1 in cells treated with OSMI-3 (Compound 2b) is observed. Because OGT knockdown is known to decrease cell proliferation, the effects of OSMI-3 on cell growth in culture over 96h is also monitored. Although there is no evidence of apoptosis, the reduced growth of cells over time is observed, consistent with the knockdown results[1].

Western Blot Analysis[1]

Cell Line: HCT116 cells
Concentration: 20 μM, 40 μM, 50 μM
Incubation Time: 4 hours, 24 hours
Result: Reduced O-GlcNAc levels.
Molecular Weight

669.77

Formula

C₃₂H₃₅N₃O₉S₂

CAS No.

2260791-13-5

SMILES

O=S(N[[email protected]](C1=CC=CC=C1OCCCC(OCC)=O)C(N(CC(OCC)=O)CC2=CC=CS2)=O)(C3=CC4=C(NC(C=C4)=O)C=C3)=O

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Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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Keywords:

OSMI-3OSMI3OSMI 3OthersOGTestercell-permeableglycosyltransferasesO-GlcNAcnucleocytoplasmicglycosylationintronsplicingsustainedInhibitorinhibitorinhibit

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