papain (USP) 

Papain (USP) refers to Papain (HY-P1645) that meets the standards of the US Pharmacopoeia. Papain is a cysteine protease of the peptidase C1 family. Papain enhances red cell agglutination by anti-D and anti-A, and increases red cell sensitivity to K cell-mediated lysis in ADCC assays. Papain can induce pulmonary emphysema. Papain can be used for the researches of Rh haemolytic disease of the newborn and pulmonary emphysema^[1][2].

Papain (5 U/mL; 15 min) pretreatment of O Rh-positive red cells enhances anti-D-mediated agglutination, while it slightly reduces anti-A-mediated agglutination of A Rh-negative red cells^[2].
Papain (5 U/mL; 15 min) pretreatment of O Rh-positive red cells increases their sensitivity to lymphocyte-mediated lysis in anti-D-mediated ADCC assays at low antibody concentrations and a 15:1 effector-to-target ratio^[2].
Papain (5 U/mL; 15 min) pretreatment of O Rh-positive target red cells enables significant PBMC-mediated lysis in anti-D-mediated ADCC assays at a 1:3:3 effector-to-target-to-competitor ratio^[2].
Papain (5 U/mL; 15 min) pretreatment of A Rh-negative target red cells enables significant PBMC-mediated lysis in anti-A-mediated ADCC assays at a 1:3:3 effector-to-target-to-competitor ratio^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Papain (10 IU; intratracheal instillatio; at day 0 and 7) induces pulmonary emphysema in female BALB/c mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

[papain (USP)]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Machado MN, et al. Bone Marrow-Derived Mononuclear Cell Therapy in Papain-Induced Experimental Pulmonary Emphysema. Front Physiol. 2018;9:121. Published 2018 Feb 20.  [Content Brief]

  [2]. Kumpel BM, et al. Comparison of lysis of bromelain and papain treated red cells in ADCC assays. Immunol Lett. 1992;31(3):237-240.  [Content Brief]