PBD dimer-2
Based on 1 Customer Validation
PBD dimer-2 (compound 2c) is a C8-linked pyrrolobenzodiazepine dimer. PBD dimer-2 can span an extra base pair and cross-link the 5′-Pu-GA(T/A)TC-Py sequence. PBD dimer-2 can be used as a payload for antibody–agent conjugates (ADCs), and it can be used for the research of cancer.
For research use only. We do not sell to patients.
- Purity: 98.01%
- CAS No.: 145325-57-1
- Formula: C31H36N4O6
- Molecular Weight:560.64
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Anti-Cancer Compound Library
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Anti-Aging Compound Library
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Anti-Lung Cancer Compound Library
HY-L075
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Anti-Blood Cancer Compound Library
HY-L079
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Anti-Hematopathy Compound Library
HY-L171
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
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Bioactive Compound Library
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High-Throughput Bioactive Compound Library
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Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| K562 | IC50 |
0.5 μM
Compound: 3c
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Inhibitory concentration against human K562 leukemia cells following incubation for 1 hour
Inhibitory concentration against human K562 leukemia cells following incubation for 1 hour
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[PMID: 14971896] |
PBD dimer-2 (Compound 16c) inhibits the growth of leukemia cell K562 with IC50 of 0.5 μM. PBD dimer-2 interferes with DNA replication and transcription by forming cross-links with DNA (50% cross-linked plasmid DNA XL50=0.07 μM)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 145325-57-1
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Appearance Solid
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Molecular Weight 560.64
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Formula C31H36N4O6
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Color White to off-white
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SMILES
O=C1C2=CC(OC)=C(OCCCCCOC3=C(C=C4C(N=C[C@@]5([H])N(CCC5)C4=O)=C3)OC)C=C2N=C[C@@]6([H])N1CCC6
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
DMSO : 130 mg/mL (231.88 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Smellie M, et al. Sequence-selective recognition of duplex DNA through covalent interstrand cross-linking: kinetic and molecular modeling studies with pyrrolobenzodiazepine dimers. Biochemistry. 2003 Jul 15;42(27):8232-9. [Content Brief]
[2]. Staben LR, et al. Systematic Variation of Pyrrolobenzodiazepine (PBD)-Dimer Payload Physicochemical Properties Impacts Efficacy and Tolerability of the Corresponding Antibody-Drug Conjugates. J Med Chem. 2020 Sep 10;63(17):9603-9622. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7837 mL | 8.9184 mL | 17.8368 mL | 44.5919 mL |
| 5 mM | 0.3567 mL | 1.7837 mL | 3.5674 mL | 8.9184 mL | |
| 10 mM | 0.1784 mL | 0.8918 mL | 1.7837 mL | 4.4592 mL | |
| 15 mM | 0.1189 mL | 0.5946 mL | 1.1891 mL | 2.9728 mL | |
| 20 mM | 0.0892 mL | 0.4459 mL | 0.8918 mL | 2.2296 mL | |
| 25 mM | 0.0713 mL | 0.3567 mL | 0.7135 mL | 1.7837 mL | |
| 30 mM | 0.0595 mL | 0.2973 mL | 0.5946 mL | 1.4864 mL | |
| 40 mM | 0.0446 mL | 0.2230 mL | 0.4459 mL | 1.1148 mL | |
| 50 mM | 0.0357 mL | 0.1784 mL | 0.3567 mL | 0.8918 mL | |
| 60 mM | 0.0297 mL | 0.1486 mL | 0.2973 mL | 0.7432 mL | |
| 80 mM | 0.0223 mL | 0.1115 mL | 0.2230 mL | 0.5574 mL | |
| 100 mM | 0.0178 mL | 0.0892 mL | 0.1784 mL | 0.4459 mL |
PBD dimer-2 Related Classifications
HY-148424 Related Classifications
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