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Phosphorylcholine  (Synonyms: Phosphocholine)

Cat. No.: HY-B2233B Purity: 98.0%
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Phosphatidylcholine is the main phospholipid component in eukaryotic biofilms. Phosphatidylcholine exists in commensal or pathogenic bacteria associated with eukaryotes in prokaryotes. Phosphorylcholine exhibits a surprising range of immunomodulatory properties.

For research use only. We do not sell to patients.

CAS No. : 3616-04-4

Size Price Stock Quantity
Oil + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in Water In-stock
Oil
100 mg In-stock
500 mg In-stock
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5 g   Get quote  

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Customer Review

Based on 12 publication(s) in Google Scholar

Other Forms of Phosphorylcholine:

Phosphorylcholine Related Antibodies

Top Publications Citing Use of Products

    Phosphorylcholine purchased from MedChemExpress. Usage Cited in: Immunity. 2025 Nov 11;58(11):2670-2684.e10.  [Abstract]

    Phosphorylcholine (PC, 10 μM; 24 h) increased HSV-1-induced cGAS-STING signaling and reduced HSV-1 replication in L929 cells.

    Phosphorylcholine purchased from MedChemExpress. Usage Cited in: Immunity. 2025 Nov 11;58(11):2670-2684.e10.  [Abstract]

    SMPDL3B (5 μM) were pre-incubated with Phosphorylcholine (PC, 100 μM) or ENPP1-1-IN-14 (100 μM) at 4°C for 30 min, and then incubated with cGAMP (100 μM) at 37°C for 3 h. The concentration of cGAMP was examined by cGAMP ELISA kit.

    Phosphorylcholine purchased from MedChemExpress. Usage Cited in: Immunity. 2025 Nov 11;58(11):2670-2684.e10.  [Abstract]

    SMPDL3B was pre-incubated with or without Phosphorylcholine (PC, 10 μM) at 4°C for 1 h, and then incubated with or without cGAMP (100 μM) at 37°C for 4 h. RAW-Lucia ISG cells were stimulated with the above reaction products (50-fold dilution) for 24 h and the ISRE reporter activity was assessed following the standard protocol.

    Phosphorylcholine purchased from MedChemExpress. Usage Cited in: Microbiome. 2025 Apr 2;13(1):90.  [Abstract]

    The pig intestines of the Phosphorylcholine (PC) (10 mg/kg; once daily for 8 days) pretreatment group exhibited mild congestion and edema in gut appearance, while the PEDV group showed obvious lesions and severe congestion and edema.

    Phosphorylcholine purchased from MedChemExpress. Usage Cited in: Microbiome. 2025 Apr 2;13(1):90.  [Abstract]

    Phosphorylcholine (PC) (10 mg/kg; once daily for 8 d) mitigated the negative impacts of intestinal virus, as evidenced by improved survival rates and body weight changes, and alleviated broken villi and extensive epithelial cell shedding in piglets.

    Phosphorylcholine purchased from MedChemExpress. Usage Cited in: Microbiome. 2025 Apr 2;13(1):90.  [Abstract]

    Phosphorylcholine (PC) (1, 5, and 10 μM; 24 h) treatment at different concentrations inhibited PEDV-induced cell death in both IPEC-J2 and Vero cells.

    Phosphorylcholine purchased from MedChemExpress. Usage Cited in: Microbiome. 2025 Apr 2;13(1):90.  [Abstract]

    Expression analysis of RIPK1, p-RIPK1, RIPK3, p-RIPK3, MLKL, p-MLKL, and PEDV-N in IPEC-J2 cells pre-treated with various Phosphorylcholine (PC) (1, 5, and 10 μM) concentrations post-PEDV infection. Phosphorylcholine (PC) (1, 5, and 10 μM) treatment reduced the phosphorylation levels of RIPK1, RIPK3, and MLKL.

    Phosphorylcholine purchased from MedChemExpress. Usage Cited in: Microbiome. 2025 Apr 2;13(1):90.  [Abstract]

    Phosphorylcholine (PC) (10 mg/kg; once daily for 8 days) pretreatment alleviated viral infection of the pig's intestines, keeping the intestinal villi and tissue structure intact, with only minor damage and epithelial cell shedding.

    Phosphorylcholine purchased from MedChemExpress. Usage Cited in: NPJ Biofilms Microbiomes. 2023 Sep 4;9(1):62.  [Abstract]

    Fecal water content and fecal Na+ content; C57BL/6 male mice were either orally administrated with 0.1 mL PBS (CONT group) or 0.1  mL Phosphorylcholine at a concentration of 2.5 mol/L (PC group) for 7 days. The results showed that that Phosphorylcholine increased fecal water and Na+ content.

    Phosphorylcholine purchased from MedChemExpress. Usage Cited in: NPJ Biofilms Microbiomes. 2023 Sep 4;9(1):62.  [Abstract]

    Colonic morphology (Scale bar = 200 μm; arrows, impaired villus). The results showed that Phosphorylcholine (Phosphocholine, 2.5 mol/L; 0.1  mL; i.g.; once daily for 7 days) protected mice from morphological damage.

    Phosphorylcholine purchased from MedChemExpress. Usage Cited in: NPJ Biofilms Microbiomes. 2023 Sep 4;9(1):62.  [Abstract]

    Phosphorylcholine (Phosphocholine, 2.5 mol/L; 0.1 mL; i.g.; once daily for 7 days) alleviated C. rodentium-induced apoptosis, as indicated by decreased caspase-3 expression (Fig. n), and epithelial proliferation, as indicated by decreased Ki67 expression (Fig. o).

    View All Endogenous Metabolite Isoform Specific Products:

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    Human Endogenous Metabolite Microbial Metabolite

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    α adrenergic receptor β adrenergic receptor

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Phosphatidylcholine is the main phospholipid component in eukaryotic biofilms. Phosphatidylcholine exists in commensal or pathogenic bacteria associated with eukaryotes in prokaryotes. Phosphorylcholine exhibits a surprising range of immunomodulatory properties[1].

    IC50 & Target

    Human Endogenous Metabolite

     

    Microbial Metabolite

     

    In Vitro

    Phosphorylcholine is a component of drug delivery micelles, which improves the efficiency of cellular internalization and increases the cytotoxicity of cytotoxins carried in micelles in HeLa cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Phosphorylcholine Related Antibodies
    In Vivo

    Phosphorylcholine (200-600 μmol/kg; ip; single dose) activates nicotinic and muscarinic cholinergic neurotransmission, stimulates the activation of α2-adrenergic receptors, and increases plasma glucagon and choline concentrations, thereby stimulating peripheral autonomic nervous system activity[3].
    Phosphorylcholine (2 μg; ip; once every two weeks for 4 months) induces the production of anti-PC and anti-oxLDL antibodies, increases mature B cells in the spleen, and reduces atherosclerotic lesions in the aortic root in mouse models[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Sprague Dawley rats model[3]
    Dosage: 200-600 μmol/kg
    Administration: ip; single dose
    Result: Increased plasma glucagon and choline concentration.
    Animal Model: apoE knockout mouse model[4]
    Dosage: 2 μg
    Administration: ip; once every two weeks for 4 months
    Result: Induced anti-PC and anti-oxLDL antibodies, reduced atherosclerotic lesions.
    Molecular Weight

    183.14

    Formula

    C5H14NO4P
    CAS No.
    Appearance

    Oil

    Color

    Colorless to light yellow

    SMILES

    O=P(OCC[N+](C)(C)C)([O-])O
    Structure Classification
    Initial Source

    a variety of prokaryotic and eukaryotic pathogens
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, stored under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

    Solvent & Solubility
    In Vitro: 

    H2O : 100 mg/mL (546.03 mM; Need ultrasonic)

    Ethanol : 4 mg/mL (21.84 mM; Need ultrasonic)

    DMSO : < 1 mg/mL (insoluble or slightly soluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 5.4603 mL 27.3015 mL 54.6030 mL
    5 mM 1.0921 mL 5.4603 mL 10.9206 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration
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    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

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    Volume (final)

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    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (546.03 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    Ethanol / H2O 1 mM 5.4603 mL 27.3015 mL 54.6030 mL 136.5076 mL
    5 mM 1.0921 mL 5.4603 mL 10.9206 mL 27.3015 mL
    10 mM 0.5460 mL 2.7302 mL 5.4603 mL 13.6508 mL
    15 mM 0.3640 mL 1.8201 mL 3.6402 mL 9.1005 mL
    20 mM 0.2730 mL 1.3651 mL 2.7302 mL 6.8254 mL
    H2O 25 mM 0.2184 mL 1.0921 mL 2.1841 mL 5.4603 mL
    30 mM 0.1820 mL 0.9101 mL 1.8201 mL 4.5503 mL
    40 mM 0.1365 mL 0.6825 mL 1.3651 mL 3.4127 mL
    50 mM 0.1092 mL 0.5460 mL 1.0921 mL 2.7302 mL
    60 mM 0.0910 mL 0.4550 mL 0.9101 mL 2.2751 mL
    80 mM 0.0683 mL 0.3413 mL 0.6825 mL 1.7063 mL
    100 mM 0.0546 mL 0.2730 mL 0.5460 mL 1.3651 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Phosphorylcholine Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Phosphorylcholine3616-04-4PhosphocholineEndogenous MetaboliteAdrenergic ReceptorBeta ReceptorInhibitorinhibitorinhibit

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