2. Metabolic Enzyme/Protease Neuronal Signaling GPCR/G Protein
  3. Endogenous Metabolite Adrenergic Receptor
  4. Phosphorylcholine chloride

Phosphorylcholine chloride  (Synonyms: Phosphocholine chloride)

Cat. No.: HY-B2233 Purity: 99.95%
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Phosphorylcholine chloride (Phosphocholine chloride) is the main phospholipid component in eukaryotic biofilms. Phosphorylcholine chloride exists in commensal or pathogenic bacteria associated with eukaryotes in prokaryotes. Phosphorylcholine chloride exhibits a surprising range of immunomodulatory properties.

For research use only. We do not sell to patients.

CAS No. : 107-73-3

Size Price Stock Quantity
Solid or liquid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
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Solution
10 mM * 1 mL in Water In-stock
Solid or liquid
500 mg In-stock
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5 g   Get quote  

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Based on 12 publication(s) in Google Scholar

Other Forms of Phosphorylcholine chloride:

Phosphorylcholine chloride Related Antibodies

Top Publications Citing Use of Products

    Phosphorylcholine chloride purchased from MedChemExpress. Usage Cited in: Immunity. 2025 Nov 11;58(11):2670-2684.e10.  [Abstract]

    Phosphorylcholine (PC, 10 μM; 24 h) increased HSV-1-induced cGAS-STING signaling and reduced HSV-1 replication in L929 cells.

    Phosphorylcholine chloride purchased from MedChemExpress. Usage Cited in: Immunity. 2025 Nov 11;58(11):2670-2684.e10.  [Abstract]

    SMPDL3B (5 μM) were pre-incubated with Phosphorylcholine (PC, 100 μM) or ENPP1-1-IN-14 (100 μM) at 4°C for 30 min, and then incubated with cGAMP (100 μM) at 37°C for 3 h. The concentration of cGAMP was examined by cGAMP ELISA kit.

    Phosphorylcholine chloride purchased from MedChemExpress. Usage Cited in: Immunity. 2025 Nov 11;58(11):2670-2684.e10.  [Abstract]

    SMPDL3B was pre-incubated with or without Phosphorylcholine (PC, 10 μM) at 4°C for 1 h, and then incubated with or without cGAMP (100 μM) at 37°C for 4 h. RAW-Lucia ISG cells were stimulated with the above reaction products (50-fold dilution) for 24 h and the ISRE reporter activity was assessed following the standard protocol.

    Phosphorylcholine chloride purchased from MedChemExpress. Usage Cited in: Microbiome. 2025 Apr 2;13(1):90.  [Abstract]

    The pig intestines of the Phosphorylcholine (PC) (10 mg/kg; once daily for 8 days) pretreatment group exhibited mild congestion and edema in gut appearance, while the PEDV group showed obvious lesions and severe congestion and edema.

    Phosphorylcholine chloride purchased from MedChemExpress. Usage Cited in: Microbiome. 2025 Apr 2;13(1):90.  [Abstract]

    Phosphorylcholine (PC) (10 mg/kg; once daily for 8 d) mitigated the negative impacts of intestinal virus, as evidenced by improved survival rates and body weight changes, and alleviated broken villi and extensive epithelial cell shedding in piglets.

    Phosphorylcholine chloride purchased from MedChemExpress. Usage Cited in: Microbiome. 2025 Apr 2;13(1):90.  [Abstract]

    Phosphorylcholine (PC) (1, 5, and 10 μM; 24 h) treatment at different concentrations inhibited PEDV-induced cell death in both IPEC-J2 and Vero cells.

    Phosphorylcholine chloride purchased from MedChemExpress. Usage Cited in: Microbiome. 2025 Apr 2;13(1):90.  [Abstract]

    Expression analysis of RIPK1, p-RIPK1, RIPK3, p-RIPK3, MLKL, p-MLKL, and PEDV-N in IPEC-J2 cells pre-treated with various Phosphorylcholine (PC) (1, 5, and 10 μM) concentrations post-PEDV infection. Phosphorylcholine (PC) (1, 5, and 10 μM) treatment reduced the phosphorylation levels of RIPK1, RIPK3, and MLKL.

    Phosphorylcholine chloride purchased from MedChemExpress. Usage Cited in: Microbiome. 2025 Apr 2;13(1):90.  [Abstract]

    Phosphorylcholine (PC) (10 mg/kg; once daily for 8 days) pretreatment alleviated viral infection of the pig's intestines, keeping the intestinal villi and tissue structure intact, with only minor damage and epithelial cell shedding.

    Phosphorylcholine chloride purchased from MedChemExpress. Usage Cited in: NPJ Biofilms Microbiomes. 2023 Sep 4;9(1):62.  [Abstract]

    Fecal water content and fecal Na+ content; C57BL/6 male mice were either orally administrated with 0.1 mL PBS (CONT group) or 0.1  mL Phosphorylcholine at a concentration of 2.5 mol/L (PC group) for 7 days. The results showed that that Phosphorylcholine increased fecal water and Na+ content.

    Phosphorylcholine chloride purchased from MedChemExpress. Usage Cited in: NPJ Biofilms Microbiomes. 2023 Sep 4;9(1):62.  [Abstract]

    Colonic morphology (Scale bar = 200 μm; arrows, impaired villus). The results showed that Phosphorylcholine (Phosphocholine, 2.5 mol/L; 0.1  mL; i.g.; once daily for 7 days) protected mice from morphological damage.

    Phosphorylcholine chloride purchased from MedChemExpress. Usage Cited in: NPJ Biofilms Microbiomes. 2023 Sep 4;9(1):62.  [Abstract]

    Phosphorylcholine (Phosphocholine, 2.5 mol/L; 0.1 mL; i.g.; once daily for 7 days) alleviated C. rodentium-induced apoptosis, as indicated by decreased caspase-3 expression (Fig. n), and epithelial proliferation, as indicated by decreased Ki67 expression (Fig. o).

    View All Endogenous Metabolite Isoform Specific Products:

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    Human Endogenous Metabolite Microbial Metabolite

    View All Adrenergic Receptor Isoform Specific Products:

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    α adrenergic receptor β adrenergic receptor

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Phosphorylcholine chloride (Phosphocholine chloride) is the main phospholipid component in eukaryotic biofilms. Phosphorylcholine chloride exists in commensal or pathogenic bacteria associated with eukaryotes in prokaryotes. Phosphorylcholine chloride exhibits a surprising range of immunomodulatory properties[1].

    IC50 & Target

    Microbial Metabolite

     

    Human Endogenous Metabolite

     

    In Vitro

    Phosphorylcholine chloride is a component of drug delivery micelles, which improves the efficiency of cellular internalization and increases the cytotoxicity of cytotoxins carried in micelles in HeLa cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Phosphorylcholine chloride Related Antibodies
    In Vivo

    Phosphorylcholine chloride (200-600 μmol/kg; ip; single dose) activates nicotinic and muscarinic cholinergic neurotransmission, stimulates the activation of α2-adrenergic receptors, and increases plasma glucagon and choline concentrations, thereby stimulating peripheral autonomic nervous system activity[3].
    Phosphorylcholine chloride (2 μg; ip; once every two weeks for 4 months) induces the production of anti-PC and anti-oxLDL antibodies, increases mature B cells in the spleen, and reduces atherosclerotic lesions in the aortic root in mouse models[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    219.60

    Formula

    C5H15ClNO4P
    CAS No.
    Appearance

    Solid-Liquid Mixture

    Color

    Colorless to light green

    SMILES

    O=P(OCC[N+](C)(C)C)(O)O.[Cl-]
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : 175 mg/mL (796.90 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.5537 mL 22.7687 mL 45.5373 mL
    5 mM 0.9107 mL 4.5537 mL 9.1075 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (455.37 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 4.5537 mL 22.7687 mL 45.5373 mL 113.8433 mL
    5 mM 0.9107 mL 4.5537 mL 9.1075 mL 22.7687 mL
    10 mM 0.4554 mL 2.2769 mL 4.5537 mL 11.3843 mL
    15 mM 0.3036 mL 1.5179 mL 3.0358 mL 7.5896 mL
    20 mM 0.2277 mL 1.1384 mL 2.2769 mL 5.6922 mL
    25 mM 0.1821 mL 0.9107 mL 1.8215 mL 4.5537 mL
    30 mM 0.1518 mL 0.7590 mL 1.5179 mL 3.7948 mL
    40 mM 0.1138 mL 0.5692 mL 1.1384 mL 2.8461 mL
    50 mM 0.0911 mL 0.4554 mL 0.9107 mL 2.2769 mL
    60 mM 0.0759 mL 0.3795 mL 0.7590 mL 1.8974 mL
    80 mM 0.0569 mL 0.2846 mL 0.5692 mL 1.4230 mL
    100 mM 0.0455 mL 0.2277 mL 0.4554 mL 1.1384 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Phosphorylcholine chloride Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Phosphorylcholine107-73-3PhosphocholineEndogenous MetaboliteAdrenergic ReceptorBeta ReceptorInhibitorinhibitorinhibit

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