2. Anti-infection Autophagy Apoptosis Neuronal Signaling Immunology/Inflammation MAPK/ERK Pathway NF-κB Epigenetics Cell Cycle/DNA Damage PI3K/Akt/mTOR Metabolic Enzyme/Protease
  3. Fungal Autophagy Apoptosis PINK1/Parkin NOD-like Receptor (NLR) Caspase JNK NF-κB Sirtuin AMPK Reactive Oxygen Species (ROS)
  4. (+)-Magnoflorine chloride

(+)-Magnoflorine chloride  (Synonyms: Magnoflorine chloride; α-Magnoflorine chloride; Thalictrine chloride)

Cat. No.: HY-N0535 Purity: 99.10%
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(+)-Magnoflorine (α-Magnoflorine) chloride is an orally active aporphine alkaloid with multiple biological activities. (+)-Magnoflorine chloride promotes Parkin/PINK1-mediated mitochondrial autophagy, inhibits the activation of NLRP3/Caspase-1 pathway, regulates the intestinal microbiota, and exhibits significant anti-inflammatory and immunomodulatory activities. (+)-Magnoflorine chloride inhibits JNK and TLR4/NF-κB signaling pathways, activates Sirt1/AMPK pathway, alleviates neuronal oxidative stress and apoptosis. Magnoflorine chloride upregulates miR-410-3p, inhibits HMGB1/NF-κB pathway, and has anti-tumor activity. (+)-Magnoflorine chloride also has significant antifungal activity.

For research use only. We do not sell to patients.

(+)-Magnoflorine chloride

(+)-Magnoflorine chloride Chemical Structure

CAS No. : 6681-18-1

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Based on 1 publication(s) in Google Scholar

Other Forms of (+)-Magnoflorine chloride:

(+)-Magnoflorine chloride Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE (+)-Magnoflorine chloride

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NLRP3 NLRP1 NOD1 NOD2

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Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

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JNK1 JNK2 JNK3 JNK

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

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Sirtuin SIRT1 SIRT2 SIRT3 SIRT6 SIRT5 SIRT7 SIRT4

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NUAK1 NUAK2 AMPK AMPK α1β1γ1 AMPK α2β1γ1 AMPK α1β2γ1 AMPK α2β2γ1 BRSK1 BRSK2 HUNK AMPKα

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

(+)-Magnoflorine (α-Magnoflorine) chloride is an orally active aporphine alkaloid with multiple biological activities. (+)-Magnoflorine chloride promotes Parkin/PINK1-mediated mitochondrial autophagy, inhibits the activation of NLRP3/Caspase-1 pathway, regulates the intestinal microbiota, and exhibits significant anti-inflammatory and immunomodulatory activities. (+)-Magnoflorine chloride inhibits JNK and TLR4/NF-κB signaling pathways, activates Sirt1/AMPK pathway, alleviates neuronal oxidative stress and apoptosis. Magnoflorine chloride upregulates miR-410-3p, inhibits HMGB1/NF-κB pathway, and has anti-tumor activity. (+)-Magnoflorine chloride also has significant antifungal activity[1][2][3][4][5][6][7].

In Vitro

(+)-Magnoflorine chloride has significant antifungal activities against Penicillium avellaneum UC-4376, T. rubrum and T. mentagrophyte with MIC values of 5 μg/disc, 62.5 μg/mL and 62.5 μg/mL, respectively[1][2].
(+)-Magnoflorine (5-80 μM, 24 h) chloride inhibit the proliferation, invasion and epithelial-mesenchymal transition process of MG-63 and U-2 OS cells[3].
(+)-Magnoflorine (10-40 μM, 24 h) chloride upregulates miR-410-3p and thereby inhibits the HMGB1/NF-κB pathway[3].
(+)-Magnoflorine (10 μM, 27 h) chloride significantly reverses the decline in PC12 cell viability caused by Aβ1-42and reduces the apoptosis rate[4].
(+)-Magnoflorine (10 μM, 27 h) chloride significantly reduces the increase in intracellular ROS levels caused by Aβ1-42, and restores the damaged mitochondrial membrane potential[4].
(+)-Magnoflorine (0-50 μM, 24 h) chloride inhibits the activation of the NLRP3 inflammasome in HK-2 cells by promoting Parkin-dependent mitochondrial autophagy[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

(+)-Magnoflorine chloride Related Antibodies

RT-PCR[3]

Cell Line: MG-63 and U-2 OS cells
Concentration: 10, 20 and 40 μM
Incubation Time: 24 h
Result: Upregulated the mRNA expression of E-cadherin in a dose-dependent manner, and downregulate the expressions of N-cadherin and Vimentin.
Dose-dependently down-regulates the mRNA expression of HMGB1.
Inhibited the activation of the NF-κB pathway resulting in a decrease in the levels of phosphorylated-p65 and phosphorylated-IκBα.
Significantly upregulated the expression of miR-410-3p in osteosarcoma cells.

Cell Invasion Assay[3]

Cell Line: MG-63 and U-2 OS cells
Concentration: 10, 20 and 40 μM
Incubation Time: 24 h
Result: Significantly reduced the number of MG-63 and U-2 OS cells passing through the matrix gel.

Western Blot Analysis[3]

Cell Line: MG-63 and U-2 OS cells
Concentration: 10, 20 and 40 μM
Incubation Time: 24 h
Result: Upregulated the protein expression of E-cadherin in a dose-dependent manner, and downregulate the expressions of N-cadherin and Vimentin.
Dose-dependently down-regulates the protein expression of HMGB1.
Inhibited the activation of the NF-κB pathway resulting in a decrease in the levels of phosphorylated-p65 and phosphorylated-IκBα.
Significantly upregulated the expression of miR-410-3p in osteosarcoma cells.

Apoptosis Analysis[4]

Cell Line: PC12 cells
Concentration: 10 μM
Incubation Time: pre-treated for 3 h, and then add Aβ1-42 for co-culture for 24 h
Result: Significantly reduced the apoptosis rate caused by Aβ1-42.
Reduced the expression of pro-apoptotic proteins Bax and Cleaved Caspase-3, and increased the expression of anti-apoptotic protein Bcl-2.
In Vivo

(+)-Magnoflorine (1-10 mg/kg, i.p., once daily for a month) chloride significantly improves the cognitive dysfunction of Alzheimer’s disease (AD) model mice[4].
(+)-Magnoflorine (10-20 mg/kg, i.g., once daily for 15 days) chloride has a protective effect on cerebral ischemia-reperfusion injury in rats[5].
(+)-Magnoflorine (10 mg/kg, i.g., once daily for 10 days) chloride relieves colitis induced by DDS (HY-116282C) in mice and the concurrent anxiety-like behaviors[6].
(+)-Magnoflorine (5-10 mg/kg, i.g., once daily for 12 weeks) chloride exhibits renal protective effects in a mouse model of chronic kidney disease induced by a high-fat and high-fructose diet[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: AD model established in APP/PS1 (APPswe/PSEN1dE9) double-transgenic mice (8 months, 30-32 g, female)[4]
Dosage: 1 and 10 mg/kg
Administration: i.p., once daily for a month
Result: Improved the learning and memory abilities of mice.
Reduced the core pathological changes in the brain, including Aβ deposition, excessive phosphorylation of Tau protein, neuroinflammation and neuronal loss.
Animal Model: Middle cerebral artery occlusion (MCAO) models model established in male Sprague-Dawley rats (weighing 220 g)[5]
Dosage: 10 and 20 mg/kg
Administration: i.g., once daily for 15 days
Result: Significantly reduced the neurological deficit score, indicating that it improved motor, balance and sensory functions.
Significantly reduced the volume of cerebral infarction and decreased the brain water content.
Reduced the number of damaged neurons shown by Nissl staining and increased the density of NeuN-positive neurons.
Reduced the upregulation of LC3 fluorescence intensity and the LC3-II/LC3-I ratio caused by cerebral ischemia, and increased the expression of p62, Sirt1 and p-AMPK proteins.
Animal Model: Colitis models model established in female C57BL/6 J mice (6-8 weeks old)[6]
Dosage: 10 mg/kg
Administration: i.g., once daily for 10 days
Result: Reduced the disease activity index, the length of the colon, the tissue pathological damage, and decreased the mRNA levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) in the colon tissue.
Significantly improved the anxiety-like behaviors induced by DSS.
Inhibited the activation of microglia (IBA1+) in the hippocampus caused by DSS.
Reduced the mRNA levels of TNF-α, IL-1β, and IL-6 in the hippocampal tissue, and upregulated the expression of tight junction proteins ZO-1 and PV1 in the choroid plexus and blood-brain barrier.
Promoted the production of secondary bile acid HDCA by the microbiota.
Animal Model: Chronic kidney disease model established in male C57BL/6 J mice (20 g)[6]
Dosage: 5 and 10 mg/kg
Administration: i.g., once daily for 12 weeks
Result: Significantly reduced the neurological deficit score, indicating that it improved motor, balance and sensory functions.
Significantly reduced the levels of serum creatinine, urea nitrogen, uric acid and elevated urine protein.
Reduced the fatty degeneration of renal tubules, lipid deposition and tubular dilation.
Reduced the collagen deposition in the kidneys, and downregulated the mRNA and protein expressions of fibrosis-related markers (TGF-β, α-SMA, COL-I, COL-III).
Reduced the levels of reactive oxygen species (ROS) and malondialdehyde (MDA) in the kidneys, and increased the activity of total superoxide dismutase (T-SOD).
Inhibited the activation of the NLRP3 inflammasome (reduced the expression of NLRP3, ASC, Cleaved Caspase-1, GSDMD-N and IL-1β).
Molecular Weight

377.86

Formula

C20H24ClNO4
CAS No.
Appearance

Solid

Color

Yellow to brown

SMILES

OC1=C(C2=C3O)C([C@]([N+]4(C)C)([H])CC2=CC=C3OC)=C(CC4)C=C1OC.[Cl-]
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : 41.67 mg/mL (110.28 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6465 mL 13.2324 mL 26.4648 mL
5 mM 0.5293 mL 2.6465 mL 5.2930 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (264.65 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.29%

SDS (393 KB)

COA (255 KB) HNMR (122 KB) LCMS (92 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.6465 mL 13.2324 mL 26.4648 mL 66.1621 mL
5 mM 0.5293 mL 2.6465 mL 5.2930 mL 13.2324 mL
10 mM 0.2646 mL 1.3232 mL 2.6465 mL 6.6162 mL
15 mM 0.1764 mL 0.8822 mL 1.7643 mL 4.4108 mL
20 mM 0.1323 mL 0.6616 mL 1.3232 mL 3.3081 mL
25 mM 0.1059 mL 0.5293 mL 1.0586 mL 2.6465 mL
30 mM 0.0882 mL 0.4411 mL 0.8822 mL 2.2054 mL
40 mM 0.0662 mL 0.3308 mL 0.6616 mL 1.6541 mL
50 mM 0.0529 mL 0.2646 mL 0.5293 mL 1.3232 mL
60 mM 0.0441 mL 0.2205 mL 0.4411 mL 1.1027 mL
80 mM 0.0331 mL 0.1654 mL 0.3308 mL 0.8270 mL
100 mM 0.0265 mL 0.1323 mL 0.2646 mL 0.6616 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(+)-Magnoflorine6681-18-1Magnoflorineα-MagnoflorineThalictrineFungalAutophagyApoptosisPINK1/ParkinNOD-like Receptor (NLR)CaspaseJNKNF-κBSirtuinAMPKReactive Oxygen Species (ROS)PTEN-induced kinase 1/Parkinc-Jun N-terminal kinaseNuclear factor-κBNuclear factor-kappaBAMP-activated protein kinaseantifungalPenicillium avellaneumT. rubrumT. mentagrophyteaporphine alkaloidInhibitorinhibitorinhibit

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