Protopine hydrochloride
Protopine hydrochloride, an isoquinoline alkaloid, is a specific reversible and competitive inhibitor of acetylcholinesterase. Protopine hydrochloride exhibits anti-inflammation, anti-microbial, anti-angiogenic and anti-tumour activity.
For research use only. We do not sell to patients.
- CAS No.: 6164-47-2
- Formula: C20H20ClNO5
- Molecular Weight:389.83
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Protopine (10-40 μM, 24 h-96 h) hydrochloride inhibits cell viability, migration, invasion and EMT process of liver carcinoma cells (HepG2, Huh7)[2].
Protopine (10-40 μM, 24 h) hydrochloride induces apoptosis by increasing the expression of caspase-3 and caspase-9 in HepG2 and Huh7 cells, and inhibits the PI3K/Akt signaling pathway[2].
Protopine (10-40 μM, 6 h) hydrochloride induces generation of ROS in HepG2 and Huh7 cells[2].
Protopine (0-10 μg/mL) hydrochloride inhibits the uptake of serotonin transporter (SERT) in S6 cells and noradrenaline (NE) uptake in N1 cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HepG2, Huh7
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Concentration:10, 20, 40 μM
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Incubation Time:24 h
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Result:Induced the cleavage of caspase-3 and caspase-9.
Decreased Bcl-2 and Bcl-xl level.
Induced the release of mitochondrial protein cytochrome c into the cytosol.
Protopine (5-20 mg/kg, i.v.) hydrochloride inhibits tumor growth and inhibits PI3K/Akt, and induces cleavage of caspase-3 in xenograft BALB/c mice (s.c. with HepG2 or Huh-7 cells)[2].
Protopine (5-20 mg/kg, i.p.) hydrochloride shows antidepressant-like effect in mice HTR and TST tests[3].
Protopine (1-4 mg/kg, i.p., once daily for 3 days) hydrochloride shows protective effect on the focal cerebral ischaemic induced injury in rats[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:5-hydroxy-DL-tryptophan (5-HTP)-induced mice model[3]
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Dosage:5, 10, 20 mg/kg
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Administration:i.p.
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Result:Increased the number of 5-HTP-induced head twitch response (HTR).
Decreased the immobility time tested in the Tail suspension test (TST).
Chemical Information
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CAS No. 6164-47-2
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Molecular Weight 389.83
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Formula C20H20ClNO5
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SMILES
O=C1CC2=C(CN(CCC3=CC4=C(C=C31)OCO4)C)C5=C(C=C2)OCO5.Cl
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Synonyms
Corydinine hydrochloride
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Kim SR, et, al. Protopine from Corydalis ternata has anticholinesterase and antiamnesic activities. Planta Med. 1999 Apr;65(3):218-21. [Content Brief]
[2]. Nie C, et, al. Protopine triggers apoptosis via the intrinsic pathway and regulation of ROS/PI3K/Akt signalling pathway in liver carcinoma. Cancer Cell Int. 2021 Jul 27;21(1):396. [Content Brief]
[3]. Xu LF, et al. Protopine inhibits serotonin transporter and noradrenaline transporter and has the antidepressant-like effect in mice models. Neuropharmacology. 2006 Jun;50(8):934-40. [Content Brief]
[4]. Xiao X, et al. Protective effect of protopine on the focal cerebral ischaemic injury in rats. Basic Clin Pharmacol Toxicol. 2007 Aug;101(2):85-9. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protopine hydrochloride Related Classifications
HY-N0793A Related Classifications
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