Protopine hydrochloride  (Synonyms: Corydinine hydrochloride)

Protopine hydrochloride, an isoquinoline alkaloid, is a specific reversible and competitive inhibitor of acetylcholinesterase. Protopine hydrochloride exhibits anti-inflammation, anti-microbial, anti-angiogenic and anti-tumour activity^[1][2].

Protopine (10-40 μM, 24 h-96 h) hydrochloride inhibits cell viability, migration, invasion and EMT process of liver carcinoma cells (HepG2, Huh7)^[2].
Protopine (10-40 μM, 24 h) hydrochloride induces apoptosis by increasing the expression of caspase-3 and caspase-9 in HepG2 and Huh7 cells, and inhibits the PI3K/Akt signaling pathway^[2].
Protopine (10-40 μM, 6 h) hydrochloride induces generation of ROS in HepG2 and Huh7 cells^[2].
Protopine (0-10 μg/mL) hydrochloride inhibits the uptake of serotonin transporter (SERT) in S6 cells and noradrenaline (NE) uptake in N1 cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Protopine (0.1 and 1 mg/kg, i.p.) hydrochloride alleviates Scopolamine (HY-N0296) (1 mg/kg)-induced memory impairment in mice^[1].
Protopine (5-20 mg/kg, i.v.) hydrochloride inhibits tumor growth and inhibits PI3K/Akt, and induces cleavage of caspase-3 in xenograft BALB/c mice (s.c. with HepG2 or Huh-7 cells)^[2].
Protopine (5-20 mg/kg, i.p.) hydrochloride shows antidepressant-like effect in mice HTR and TST tests^[3].
Protopine (1-4 mg/kg, i.p., once daily for 3 days) hydrochloride shows protective effect on the focal cerebral ischaemic induced injury in rats^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

389.83

C₂₀H₂₀ClNO₅

6164-47-2

O=C1CC2=C(CN(CCC3=CC4=C(C=C31)OCO4)C)C5=C(C=C2)OCO5.Cl

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Kim SR, et, al. Protopine from Corydalis ternata has anticholinesterase and antiamnesic activities. Planta Med. 1999 Apr;65(3):218-21.  [Content Brief]

  [2]. Nie C, et, al. Protopine triggers apoptosis via the intrinsic pathway and regulation of ROS/PI3K/Akt signalling pathway in liver carcinoma. Cancer Cell Int. 2021 Jul 27;21(1):396.  [Content Brief]

  [3]. Xu LF, et al. Protopine inhibits serotonin transporter and noradrenaline transporter and has the antidepressant-like effect in mice models. Neuropharmacology. 2006 Jun;50(8):934-40.  [Content Brief]

  [4]. Xiao X, et al. Protective effect of protopine on the focal cerebral ischaemic injury in rats. Basic Clin Pharmacol Toxicol. 2007 Aug;101(2):85-9.  [Content Brief]