RP-4029
RP-4029 is a selective, covalent hPolθ inhibitor (IC50s: 0.013 nM for hPolθ; 6.6 nM for mPolθ). RP-4029 can be used in the research of homologous recombination (HR)-deficient cancers.
For research use only. We do not sell to patients.
- Formula: C29H23F6N3O4S
- Molecular Weight:623.57
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
All DNA/RNA Synthesis Isoforms
More
Biological Activity
Description
IC50 & Target
IC50s: 0.013 nM for hPolθ; 6.6 nM for mPolθ[1]
Chemical Information
-
Molecular Weight 623.57
-
Formula C29H23F6N3O4S
-
SMILES
O=C(N(C1=CC=CC=C1)CC#CCNC(C2=C(NS(C=C)(=O)=O)C=CC=C2)=O)CC3=CC=C(C(F)(F)F)C=C3C(F)(F)F
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
-
CRISPR-Cas9 HDR knock-in/precise editing
CRISPR-Cas9 HDR knock-in uses a guide RNA to direct Cas9 to a genomic target adjacent to a PAM, where Cas9 creates a double-strand break; if a donor DNA template with homology to the cut region is present, cellular HDR can copy the donor sequence into the genome, producing a precise substitution, tag, reporter, or insertion rather than an indel. The readout is the fraction of alleles or cells carrying the intended donor-derived edit, measured by junction PCR, restriction-fragment analysis, Sanger sequencing, amplicon deep sequencing, flow cytometry for reporter knock-in, or clone genotyping; NHEJ indels and partial or non-HDR insertions are measured in parallel because they compete with or confound precise HDR outcomes.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
RP-4029 Related Classifications
HY-181026 Related Classifications
Powered by Bioz