2. Apoptosis
  3. Caspase Apoptosis
  4. Ru(acac)3

Ru(acac)3  (Synonyms: Tris(acetylacetonato)ruthenium (III))

Cat. No.: HY-W020958 Purity: 99.9%
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Ru (acac) 3 (Tris (acetylacetonato) ruthenium (III)) is a caspase-3 activator and Apoptosis inducer. Ru (acac) 3 exerts growth inhibitory effects on various cell lines in vitro by inhibiting DNA/RNA synthesis and inducing mild reversible S-phase cell cycle arrest. Ru (acac) 3 is commonly used in research related to ovarian cancer, osteosarcoma, cervical cancer, melanoma, and other fields.

For research use only. We do not sell to patients.

Ru(acac)3

Ru(acac)3 Chemical Structure

CAS No. : 14284-93-6

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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1 g In-stock
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Based on 1 publication(s) in Google Scholar

Ru(acac)3 Related Antibodies

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Description

Ru (acac) 3 (Tris (acetylacetonato) ruthenium (III)) is a caspase-3 activator and Apoptosis inducer. Ru (acac) 3 exerts growth inhibitory effects on various cell lines in vitro by inhibiting DNA/RNA synthesis and inducing mild reversible S-phase cell cycle arrest. Ru (acac) 3 is commonly used in research related to ovarian cancer, osteosarcoma, cervical cancer, melanoma, and other fields[1][2].

In Vitro

Ru (acac)3 (100 μM; 24 h) does not inhibit the growth of A2780, U2-OS or U2-OS/Pt cells at concentrations up to 100 μM, with an IC50 value greater than 100 μM[1].
Ru (acac) 3 (treated with U2-OS and U2-OS/Pt cells for 48 h, and HeLa and B16 cells for 72 h; 300 μM) inhibits the growth of HeLa, B16 and U2-OS cells, with IC50 values ranging from 114 μM to 173 μM; it shows no detectable activity against U2-OS/Pt cells at concentrations up to 300 μM[1].
Ru (acac)3 (100 μM; 24 h, 48 h) induces apoptosis in A2780 cells, with the proportion of apoptotic cells reaching 9.93% after 24 h and 14.70% after 48 h[1].
Ru (acac)3 (IC50, 1.5×IC50; 48 h, 72 h) induces a mild, time-dependent decrease in the proportion of G1- and G2-phase cells in HeLa cells, and increases the proportion of apoptotic sub-G1-phase cells to approximately 10% after 72 h[1].
Treatment with Ru (acac)3 (1.5×IC50-10×IC50; 5-48 h) for 24 h induces apoptosis in 7.48% of HeLa cells and activates caspase-3[1].
Ru (acac)3 (30-100 μM; 6-24 h) reversibly inhibits RNA synthesis and suppresses DNA synthesis in a time- and concentration-dependent manner in A2780 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ru(acac)3 Related Antibodies

Cell Proliferation Assay[1]

Cell Line: human cervix carcinoma HeLa cells, murine melanoma B16 cells, human osteosarcoma U2-OS cells, human osteosarcoma CDDP-resistant U2-OS/Pt cells
Concentration: Up to 300 μM
Incubation Time: 48 h (U2-OS and U2-OS/Pt); 72 h (HeLa and B16)
Result: Exhibited IC50 values of 114 μM for HeLa cells, 148 μM for B16 cells, 173 μM for U2-OS cells, and >300 μM for U2-OS/Pt cells.
Showed that U2-OS cells had slightly higher sensitivity than U2-OS/Pt cells.

Cell Cycle Analysis[1]

Cell Line: human cervix carcinoma HeLa cells
Concentration: IC50; 1.5×IC50
Incubation Time: 48 h; 72 h
Result: Caused a time- and concentration-dependent slight decrease in the percentage of cells in G1 and G2 phases, with a corresponding increase in sub-G1 (apoptotic) cells reaching ~10% after 72 h incubation at 1.5×IC50.

Apoptosis Analysis[1]

Cell Line: human cervix carcinoma HeLa cells
Concentration: 1.5×IC50
Incubation Time: 24 h; 48 h
Result: Induced apoptosis in 7.48% of cells after 24 h exposure and 29.09% of cells after 48 h exposure.
Showed that 31.78% of cells were PI-positive after 48 h, indicating necrotic involvement in cell death.
Molecular Weight

398.39

Formula

C15H21O6Ru
CAS No.
Appearance

Solid

Color

Red to reddish brown

SMILES

CC1=CC(C)=O[Ru]O1.[3]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

RT, protect from light, stored under nitrogen

In solvent -80°C 1 year
-20°C 6 months
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (125.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5101 mL 12.5505 mL 25.1010 mL
5 mM 0.5020 mL 2.5101 mL 5.0202 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

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Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.9%

SDS (480 KB)

COA (255 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.5101 mL 12.5505 mL 25.1010 mL 62.7526 mL
5 mM 0.5020 mL 2.5101 mL 5.0202 mL 12.5505 mL
10 mM 0.2510 mL 1.2551 mL 2.5101 mL 6.2753 mL
15 mM 0.1673 mL 0.8367 mL 1.6734 mL 4.1835 mL
20 mM 0.1255 mL 0.6275 mL 1.2551 mL 3.1376 mL
25 mM 0.1004 mL 0.5020 mL 1.0040 mL 2.5101 mL
30 mM 0.0837 mL 0.4184 mL 0.8367 mL 2.0918 mL
40 mM 0.0628 mL 0.3138 mL 0.6275 mL 1.5688 mL
50 mM 0.0502 mL 0.2510 mL 0.5020 mL 1.2551 mL
60 mM 0.0418 mL 0.2092 mL 0.4184 mL 1.0459 mL
80 mM 0.0314 mL 0.1569 mL 0.3138 mL 0.7844 mL
100 mM 0.0251 mL 0.1255 mL 0.2510 mL 0.6275 mL
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Ru(acac)3 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ru(acac)314284-93-6Tris(acetylacetonato)ruthenium (III)CaspaseApoptosisovarian carcinomacaspase-3S-phase cell cycle arrestcervix carcinomamelanomaapoptosisosteosarcomaDNA/RNA synthesiscell linesHeLa cellsInhibitorinhibitorinhibit

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