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Results for "

FRET

" in MedChemExpress (MCE) Product Catalog:

By Targets:	ADAMTS (1) Adrenergic Receptor (1) Androgen Receptor (1) Angiotensin Receptor (1) Arrestin (1) Atg4 (1) Autophagy (3) Bacterial (3) Bcl-2 Family (4) Beta-secretase (1) Biochemical Assay Reagents (5) C-type Lectin-like Receptors (CTLRs) (1) Cathepsin (4) CDK (1) CHIKV (1) Cholinesterase (ChE) (1) CMV (1) DNA Alkylator/Crosslinker (1) DNA Stain (2) DNA/RNA Synthesis (1) Epigenetic Reader Domain (8) Estrogen Receptor/ERR (2) Exosomes (1) Fluorescent Dye (33) FXR (5) G protein-coupled Bile Acid Receptor 1 (1) GLP Receptor (1) Histone Acetyltransferase (8) Histone Demethylase (1) Histone Methyltransferase (2) HIV (1) Isotope-Labeled Compounds (1) Keap1-Nrf2 (2) Ligands for E3 Ligase (3) Lipocalin Family (1) Liposome (1) LXR (1) Mitophagy (1) MMP (1) Parasite (1) Reactive Oxygen Species (ROS) (1) Reference Standards (6) ROR (2) SARS-CoV (3) Ser/Thr Protease (1) STING (1) Tau Protein (1) TNF Receptor (1) Virus Protease (2) YAP (1)
By Research Areas:	Cancer (25) Cardiovascular Disease (1) Endocrinology (1) Infection (8) Inflammation/Immunology (14) Metabolic Disease (8) Neurological Disease (3)
Click Chemistry:	DBCO (1) Azide (1) Alkynes (1)
Oligonucleotides:	Fluorescent Lipids (1) Pegylated Lipids (1)

100

Inhibitors & Agonists

Fluorescent Dye

Biochemical Assay Reagents

Peptides

Inhibitory Antibodies

Isotope-Labeled Compounds

Click Chemistry

Oligonucleotides

Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-101533

AZD-5991 Maximum Cited Publications 19 Publications Verification	Bcl-2 Family	Cancer
AZD-5991 is a potent and selective Mcl-1 inhibitor with an IC₅₀ of 0.7 nM in FRET assay and a K_d of 0.17 nM in surface plasmon resonance (SPR) assay .

HY-P5354

FRETS-VWF73 1 Publications Verification	Fluorescent Dye	Cardiovascular Disease
FRETS-VWF73, a 73-amino-acid peptide, is a fluorogenic substrate for ADAMTS13 assay (Ex = 340 nm; Em = 450 nm). FRETS-VWF73 is a predictive tool for thrombotic thrombocytopenic purpura .

HY-108435

GNE-049 5+ Cited Publications	Epigenetic Reader Domain Histone Acetyltransferase	Cancer
GNE-049 is a highly potent and selective CBP inhibitor with an IC₅₀ of 1.1 nM in TR-FRET assay. GNE-049 also inhibits BRET and BRD4(1) with IC₅₀s of 12 nM and 4200 nM, respectively .

HY-15943

6-TAMRA 1 Publications Verification 6-Carboxytetramethylrhodamine	Fluorescent Dye	Others
6-TAMRA (6-Carboxytetramethylrhodamine) is a fluorescent dye. 6-TAMRA is often used in combination with 6-FAM (HY-66021) as an acceptor or fluorescence quencher for fluorescence resonance energy transfer (FRET) in biochemical studies such as potassium ion detection and ribonuclease activity assays .

HY-108696

GNE-781 5 Publications Verification	Epigenetic Reader Domain Histone Acetyltransferase	Cancer
GNE-781 is an orally active, highly potent and selective CBP inhibitor with an IC₅₀ of 0.94 nM in TR-FRET assay. GNE-781 also inhibits BRET and BRD4(1) with IC₅₀s of 6.2 nM and 5100 nM, respectively. GNE-781 displays antitumor activity in an MOLM-16 AML xenograft model .

HY-162289

FAZ-3780 2 Publications Verification	DNA/RNA Synthesis	Neurological Disease Cancer
FAZ-3780 is an inhibitor that binds to the NTF2L domain of G3BP1/2. FAZ-3780 binds to the NTF2L nsP3 of G3BP1 with high affinity, with a K_d of 0.15 μM and a Pep-FRET IC₅₀ of 0.7 μM. FAZ-3780 targets the protein-protein interaction domain of G3BP1/2, and specifically inhibits the co-condensation of G3BP1, caprin 1 and RNA in vitro. FAZ-3780 inhibits stress granule formation and disassembles pre-formed stress granules. FAZ-3780 can be used in studies related to cancer and neurodegenerative diseases .

HY-W027544

MCA 7-Methoxycoumarin-4-acetic acid	Fluorescent Dye	Others
MCA (7-Methoxycoumarin-4-acetic acid) is a Coumarin (HY-N0709) derivative. MCA quantitates platelet-activating factor (PAF) by high-performance liquid chromatography with fluorescent detection. MCA can modify FRET peptide substrates for analyzing protease activities .

HY-101027

GSK 4027 2 Publications Verification	Epigenetic Reader Domain Histone Acetyltransferase	Inflammation/Immunology Cancer
GSK 4027 is a chemical probe for the PCAF/GCN5 bromodomain with an pIC₅₀ of 7.4±0.11 for PCAF in a time-resolved fluorescence resonance energy transfer (TR-FRET) assay.

HY-W800703

Alexa Fluor 594 azide	Fluorescent Dye	Others
Alexa Fluor 594 azide is an azide-modified fluorescent dye and also a *FRET* acceptor reactant. Owing to its negligible direct absorption under 488 nm excitation light, excellent photostability, and the ability of its azide group to participate in the CuAAC reaction, Alexa Fluor 594 azide can be paired with a donor dye for single-molecule imaging via TIRFM, thereby enabling the visual monitoring of CuNP-catalyzed click reactions .

HY-D2365

QSY 21 NHS	Fluorescent Dye	Others
QSY 21 NHS is a dark quencher and an efficient energy transfer acceptor for far-red and near-infrared fluorescent probes. QSY 21 NHS operates at a wavelength range of 540-750 nm and is commonly used in FRET applications. QSY 21 NHS does not fluoresce under normal conditions. The NHS ester can be used to label primary amines (R-NH₂) of proteins, amine-modified oligonucleotides and other amine-containing molecules. QSY 21 NHS can be conjugated with dendritic poly-L-lysine to achieve intramolecular quenching of Cy5 fluorescence .

HY-152231

BODIPY FL thalidomide	Fluorescent Dye Ligands for E3 Ligase	Others
BODIPY FL thalidomide is a high-affinity and selective human cereblon (CRBN)-targeting fluorescent probe with a K_d of 3.6 nM. BODIPY FL thalidomide enables use as a probe in time-resolved fluorescence resonance energy transfer (TR-FRET) binding assays for cereblon ligands. BODIPY FL thalidomide supports development of highly sensitive, selective, stable cereblon TR-FRET binding assays (Ex/Em = 502/510 nm) .

HY-135710

ADAMTS-5-IN-2	ADAMTS Bacterial	Infection Inflammation/Immunology
ADAMTS-5-IN-2 is an inhibitor of ADAMTS-5 (IC₅₀: 9.6 μM) and BoNT/A LC (IC₅₀: 0.71 μM). ADAMTS-5-IN-2 can be used in research related to botulism, osteoarthritis, and other conditions .

HY-100522

FMK 9a 2 Publications Verification	Atg4 Autophagy	Cancer
FMK 9a is an autophagin-1 inhibitor with IC₅₀ values of 80 and 73 μM in FRET and LRA assay.

HY-P99900

Sevirumab MSL-109	CMV	Infection
Sevirumab (MSL-109) is a human IgG1 neutralizing monoclonal antibody against cytomegalovirus (CMV). Sevirumab recognizes CMV gH complexes and inhibits CMV replication with an EC₅₀ of 0.3 μg/mL .

HY-101027A

GSK4028 1 Publications Verification	Epigenetic Reader Domain Histone Acetyltransferase	Inflammation/Immunology Cancer
GSK4028 is the enantiomeric negative control of GSK4027, which is a PCAF/GCN5 bromodomain chemical probe, the pIC₅₀ of GSK4028 is 4.9 in a time-resolved fluorescence resonance energy transfer (TR-FRET) assay.

HY-A0298

EZH2-IN-2	Histone Methyltransferase	Cancer
EZH2-IN-2 (Example 69) is a EZH2 inhibitor with an IC₅₀ and a *TR-FRET* IC₅₀** of 64 nM and 20 nM. EZH2-IN-2 can be used for the research of cancer or precancerous condition related to EZH2 activity .

HY-139561

KDM2B-IN-2	Histone Demethylase	Inflammation/Immunology Cancer
KDM2B-IN-2, a potent histone demethylase (kdm2b) inhibitor with an IC₅₀ of 0.021 μM in a KDM2B TR-FRET assay. KDM2B-IN-2 can be used for hyperproliferative diseases research .

HY-102075A

TMP778	ROR	Inflammation/Immunology
TMP778 is a potent and selevtive RORγt inverse agonist, with an IC₅₀ of 7 nM in FRET assay.

HY-P5354A

FRETS-VWF73 TFA 1 Publications Verification	Fluorescent Dye	Others
FRETS-VWF73 TFA, a 73-amino-acid peptide, is a fluorogenic substrate for ADAMTS13 assay (Ex=340 nm; Em=450 nm) .

HY-42984

BHQ-2 NHS	Biochemical Assay Reagents	Others
BHQ-2 NHS is a dark quencher with no native emission due to the polyaromatic-azo backbone and a terminal NHS ester. UBHQ-2 NHS has a wide and intense quenching range from 560-670 nm, which makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with orange to far-red emitting dyes. The NHS ester can be applied to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.

HY-D1773

YOYO-3	Fluorescent Dye DNA Alkylator/Crosslinker	Others
YOYO-3 is a trimethine cyanine homodimer dye. YOYO-3 intercalates into AF488-tagged dsDNA molecules at the single-molecule level. YOYO-3 has a greater affinity for DNA than do EB and other Cyanine dyes .

HY-P2498A

*Cathepsin D and E FRET* Substrate acetate**	Cathepsin	Cancer
Cathepsin D and E FRET Substrate acetate is a fluorogenic substrate for cathepsins D and E and not for B, H or L. The cleavage occurs at the Phe-Phe amide bond resul. Cathepsin D and E FRET Substrate is a valuable tool for routine assays and for mechanistic studies on cathepsins E and D .

HY-P3792

Mca-Pro-Leu-Gly-Pro-D-Lys(Dnp)	Biochemical Assay Reagents	Others
Mca-Pro-Leu-Gly-Pro-D-Lys(Dnp) is a FRET substrate of Thimet oligopeptidase. Mca-Pro-Leu-Gly-Pro-D-Lys(Dnp) can be used for the determination of Thimet oligopeptidase activity .

HY-151412

T-271	Mitophagy	Neurological Disease
T-271 is a selective inducer of mitochondrial autophagy .

HY-D1682

NFQ1	Fluorescent Dye	Others
NFQ1 is a non-fluorescent quencher (absorption wavelength: 474 nm), and is used for a new type of One Sample Fluorescence Resonance Energy Transfer (OS-FRET) method. OS-FRET enable measurement of unquenched donor emission in the same sample. NFQ1 shows broad absorption spectrum ensuring its utility as a dark acceptor for many donors .

HY-D0053A

6-ROX hydrochloride 6-Carboxy-X-rhodamine hydrochloride	Fluorescent Dye	Others
6-ROX (6-Carboxy-X-rhodamine) hydrochloride, a fluorescent marker of oligonucleotides, acts as a receptor coupled to 5-FAM and as a donor in FRET imaging. Excitation wavelength: 568 nm. Emission wavelength: 568 nm.

HY-155312

NKG2D-IN-2	C-type Lectin-like Receptors (CTLRs)	Cancer
NKG2D-IN-2 (compound 47) is a natural killer group 2D receptor (NKG2D) inhibitor with IC₅₀s of 0.1 µM and 0.2 µM in NKG2D/MICA and ULBP6 TR-FRET assays, respectively .

HY-D1050

DNP-X, SE 1 Publications Verification 6-(2,4-Dinitrophenyl)aminohexanoic acid, succinimidyl ester	Fluorescent Dye	Metabolic Disease
DNP-X, SE (6-(2,4-Dinitrophenyl)aminohexanoic acid, succinimidyl ester), the DNP-X acid modified by succinimidyl ester, is an amine-reactive building block for developing a probe, which can be recognized by anti-DNP antibodies. DNP-X, SE (6-(2,4-Dinitrophenyl)aminohexanoic acid, succinimidyl ester) is also an excellent amine-reactive FRET quencher paired with Trp or Tyr.

HY-P5485

Dabcyl-QALPETGEE-Edans	Bacterial	Others
Dabcyl-QALPETGEE-Edans is a synthetic peptide substrate for fluorescence resonance energy transfer (FRET) detection of Sortase A (SrtA) enzymatic activity. Dabcyl-QALPETGEE-Edans is labeled at both ends with the fluorescent group Edans (donor) and the quencher Dabcyl (acceptor), respectively. Dabcyl-QALPETGEE-Edans's central sequence contains a conserved motif specifically recognized by SrtA. Dabcyl-QALPETGEE-Edans demonstrates high sensitivity in vitro assays, enabling precise determination of the half-maximal inhibitory concentration of SrtA .

HY-D2413

BODIPY FL VH032 BODIPY FL PEG4-VH032	Fluorescent Dye	Others
BODIPY FL VH032 is a high-affinity VHL fluorescent probe with a K_d value of 3.01 nM. BODIPY FL VH032 consists of von Hippel-Lindau (VHL) ligand VH032 (HY-120217) and BODIPY FL (HY-43520). BODIPY FL VH032 can be used for time-resolved fluorescence resonance energy transfer (TR-FRET) detection for high-throughput identification and characterization of VHL ligands with maximum excitation emission wavelength: 504/520 nm .

HY-P1859

MCA-SEVNLDAEFR-K(Dnp)-RR, amide	Fluorescent Dye	Others
MCA-SEVNLDAEFR-K(Dnp)-RR, amide is a FRET-based substrate.

HY-101533A

AZD-5991 Racemate	Bcl-2 Family	Cancer
AZD-5991 Racemate is the racemate of AZD-5991. AZD-5991 Racemate is a Mcl-1 inhibitor with an IC₅₀ of <3 nM in FRET assay.

HY-161159

DMHBO+ 1 Publications Verification	Fluorescent Dye	Others
DMHBO+ is a cationic chromophore that binds to the Chili aptamer with a K_d of 12 nM. The Chili-DMHBO+ complex is an ideal fluorescence donor for FRET to the rhodamine dye Atto 590, suitable for imaging RNA in cells. Ex/Em=456/592 nm .

HY-157558

KDRLKZ-1 8SGE	Ligands for E3 Ligase	Others
KDRLKZ-1 is a KLHDC2 ligand (K_d = 0.36 μM), with IC₅₀s of 0.21 μM and 0.31 μM in alphaLISA and time-resolved fluorescence resonance energy transfer (TR-FRET) displacement assays .

HY-101533B

AZD-5991 (S-enantiomer)	Bcl-2 Family	Cancer
AZD-5991 S-enantiomer is the less active enantiomer of AZD-5991. AZD-5991 S-enantiomer is a Mcl-1 inhibitor with an IC₅₀ of 6.3 μM in FRET assay and a K_d of 0.98 μM in surface plasmon resonance (SPR) assay.

HY-P5419A

WAAG-3R TFA	Biochemical Assay Reagents	Others
WAAG-3R TFA is a biological active peptide. (Aggrecanases belong to the ADAMTS (A disintegrin and metalloprotease with thrombospondin motif) family of proteases. Aggrecanases cleave aggrecan, the major structural component of cartilage. Aggrecanase-1 (ADAMTS-4) is a major aggrecanase in human osteoarthritic cartilage. This FRET peptide was used in an ADAMTS-4 (Aggrecanase-1) and ADAMTS-5 (Aggrecanase-2) assay . (Ex/Em = 340/420 nm)

HY-P2498

*Cathepsin D and E FRET* Substrate**	Cathepsin	Others
Cathepsin D and E FRET Substrate is a fluorogenic substrate for cathepsins D and E and not for B, H or L. The cleavage occurs at the Phe-Phe amide bond resul. Cathepsin D and E FRET Substrate is a valuable tool for routine assays and for mechanistic studies on cathepsins E and D .

HY-100443A

trans-PX20606 3 Publications Verification trans-PX-102	FXR Autophagy	Metabolic Disease
PX20606 trans racemate (PX-102 trans racemate) is a FXR agonist with EC₅₀s of 32 and 34 nM for FXR in FRET and M1H assay, respectively .

HY-D2274

EZH2-AF647	Fluorescent Dye	Others
EZH2-AF647 is a fluorescent probe derived from UNC2239 that improves receptor TR-FRET properties by using Alexa 647 dye .

HY-D1079

EDANS sodium	DNA Stain	Others
EDANS sodium is a potent fluorogenic substrates. EDANS sodium is a donor for FRET-based nucleic acid probes and protease substrates. EDANS sodium is often paired with DABCYL or DABSYL. The optimal absorbance and emission wavelengths of EDANS sodium are λ_abs = 336 nm and λ_em = 490 nm respectively .

HY-D2167

AF 568 alkyne	Fluorescent Dye	Others
AF 568 alkyne is a derivative of the orange fluorescent dye AF 568. AF 568 has an absorption wavelength of 590-720 nm (FRET) and an emission wavelength of 515-720 nm. AF 568 alkyne can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) via the Alkyne group and molecules containing Azide groups. To achieve specific coupling of dye labels and biomolecules .

HY-173308

QSY-21	Fluorescent Dye	Others
QSY-21 is a fluorescence quencher. QSY-21 possesses broad absorption in far red and NIR range, and can quench fluorescence of dyes that emit in this region. This is a carboxylic acid derivative. QSY-21 has intense absorption maximum at 661 nm, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications. It is a common quencher for Cyanine5, Cyanine5.5, AF 647, or other spectrally similar fluorescent dyes.

HY-P11302

DABCYL-RAGGYIFS-(E-EDANS)-NH2	CHIKV Virus Protease	Infection
DABCYL-RAGGYIFS-(E-EDANS)-NH2, an octapeptide, is a FRET-based substrate peptide of CHIKV nsP2protease with highly sensitivity. DABCYL-RAGGYIFS-(E-EDANS)-NH2 can be cleaved by the active CHIKV nsP2protease, separating fluorophore and quencher and resulting in an increase of fluorescence signal. DABCYL-RAGGYIFS-(E-EDANS)-NH2 can used for the determination of CHIKV nsP2protease activity .

HY-161352

NPI52	Virus Protease	Infection
NPI52 (Compound E1) is a foot-and-mouth disease virus 3C protease (FMDV 3Cpro) inhibitor, with IC₅₀ values of 0.05 and 0.11 µM in FRET and cell-based assay, respectively .

HY-P10344

MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2	Fluorescent Dye	Infection
MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 is a fluorescent substrate used for fluorescence resonance energy transfer (FRET) protease assays. This substrate is employed to measure the activity of SARS-CoV-2 3CLpro .

HY-144127

AR antagonist 3	Androgen Receptor	Cancer
AR antagonist 3 is a potent and selective androgen receptor (AR) antagonist with an IC₅₀ of 0.47 µM. AR antagonist 3 exhibits a dose-dependent decrease of the FRET signal (IC₅₀= 18.05 μM). AR antagonist 3 shows effective inhibition on tumor growth when administered intratumorally .

HY-148643

STING modulator-5	STING	Inflammation/Immunology
STING modulator-5 (compound 38) is a STING modulator with a pIC₅₀ value of 9.5. STING modulator-5 antagonizes peripheral blood mononuclear cells (PBMC) with a pIC₅₀ value of 8.1. STING modulator-5 is a antagonist of THP-1 cells, it can be used for immunological disease research .

HY-P4787A

Ac-Glu-Asp(EDANS)-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Gly-Lys(DABCYL)-Glu-NH2 acetate	Cathepsin	Others
Ac-Glu-Asp(EDANS)-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Gly-Lys(DABCYL)-Glu-NH2 acetate is a Cathepsin D substrate, that can be used for cathepsin D FRET assay .

HY-P4787

Ac-Glu-Asp(EDANS)-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Gly-Lys(DABCYL)-Glu-NH2	Cathepsin	Others
Ac-Glu-Asp(EDANS)-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Gly-Lys(DABCYL)-Glu-NH2 is a cathepsin D substrate, that can be used for cathepsin D FRET assay .

HY-176955

ER degrader 12	Estrogen Receptor/ERR	Cancer
ER degrader 12 (Example 1) is an estrogen receptor (ER) degrader with IC₅₀ values for ERα and ERβ of 2.3 and 80.2 nM respectively (TR-FRET experiment). ER degrader 12 inhibits the proliferation of MCF-7 cells, with an IC₅₀ of 1.53 nM. ER degrader 12 can be used for research on breast cancer .

Cat. No.	Product Name	Type

HY-15943

6-TAMRA 1 Publications Verification 6-Carboxytetramethylrhodamine	Fluorescent Dye
6-TAMRA (6-Carboxytetramethylrhodamine) is a fluorescent dye. 6-TAMRA is often used in combination with 6-FAM (HY-66021) as an acceptor or fluorescence quencher for fluorescence resonance energy transfer (FRET) in biochemical studies such as potassium ion detection and ribonuclease activity assays .

HY-W027544

MCA 7-Methoxycoumarin-4-acetic acid	Fluorescent Dye
MCA (7-Methoxycoumarin-4-acetic acid) is a Coumarin (HY-N0709) derivative. MCA quantitates platelet-activating factor (PAF) by high-performance liquid chromatography with fluorescent detection. MCA can modify FRET peptide substrates for analyzing protease activities .

HY-D2365

QSY 21 NHS

Fluorescent Dye

QSY 21 NHS is a dark quencher and an efficient energy transfer acceptor for far-red and near-infrared fluorescent probes. QSY 21 NHS operates at a wavelength range of 540-750 nm and is commonly used in FRET applications. QSY 21 NHS does not fluoresce under normal conditions. The NHS ester can be used to label primary amines (R-NH₂) of proteins, amine-modified oligonucleotides and other amine-containing molecules. QSY 21 NHS can be conjugated with dendritic poly-L-lysine to achieve intramolecular quenching of Cy5 fluorescence .

HY-42984

BHQ-2 NHS

Fluorescent Dye

BHQ-2 NHS is a dark quencher with no native emission due to the polyaromatic-azo backbone and a terminal NHS ester. UBHQ-2 NHS has a wide and intense quenching range from 560-670 nm, which makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with orange to far-red emitting dyes. The NHS ester can be applied to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.

HY-D1773

YOYO-3	Fluorescent Dye
YOYO-3 is a trimethine cyanine homodimer dye. YOYO-3 intercalates into AF488-tagged dsDNA molecules at the single-molecule level. YOYO-3 has a greater affinity for DNA than do EB and other Cyanine dyes .

HY-D1682

NFQ1	Fluorescent Dye
NFQ1 is a non-fluorescent quencher (absorption wavelength: 474 nm), and is used for a new type of One Sample Fluorescence Resonance Energy Transfer (OS-FRET) method. OS-FRET enable measurement of unquenched donor emission in the same sample. NFQ1 shows broad absorption spectrum ensuring its utility as a dark acceptor for many donors .

HY-D1050

DNP-X, SE 1 Publications Verification 6-(2,4-Dinitrophenyl)aminohexanoic acid, succinimidyl ester	Fluorescent Dye
DNP-X, SE (6-(2,4-Dinitrophenyl)aminohexanoic acid, succinimidyl ester), the DNP-X acid modified by succinimidyl ester, is an amine-reactive building block for developing a probe, which can be recognized by anti-DNP antibodies. DNP-X, SE (6-(2,4-Dinitrophenyl)aminohexanoic acid, succinimidyl ester) is also an excellent amine-reactive FRET quencher paired with Trp or Tyr.

HY-D2413

BODIPY FL VH032

BODIPY FL PEG4-VH032

Fluorescent Dye

BODIPY FL VH032 is a high-affinity VHL fluorescent probe with a K_d value of 3.01 nM. BODIPY FL VH032 consists of von Hippel-Lindau (VHL) ligand VH032 (HY-120217) and BODIPY FL (HY-43520). BODIPY FL VH032 can be used for time-resolved fluorescence resonance energy transfer (TR-FRET) detection for high-throughput identification and characterization of VHL ligands with maximum excitation emission wavelength: 504/520 nm .

HY-D2274

EZH2-AF647	Fluorescent Dye
EZH2-AF647 is a fluorescent probe derived from UNC2239 that improves receptor TR-FRET properties by using Alexa 647 dye .

HY-D2167

AF 568 alkyne	Fluorescent Dye
AF 568 alkyne is a derivative of the orange fluorescent dye AF 568. AF 568 has an absorption wavelength of 590-720 nm (FRET) and an emission wavelength of 515-720 nm. AF 568 alkyne can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) via the Alkyne group and molecules containing Azide groups. To achieve specific coupling of dye labels and biomolecules .

HY-173308

QSY-21

Fluorescent Dye

QSY-21 is a fluorescence quencher. QSY-21 possesses broad absorption in far red and NIR range, and can quench fluorescence of dyes that emit in this region. This is a carboxylic acid derivative. QSY-21 has intense absorption maximum at 661 nm, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications. It is a common quencher for Cyanine5, Cyanine5.5, AF 647, or other spectrally similar fluorescent dyes.

HY-D2735

QSY-9-succinimidyl ester

Fluorescent Dye

QSY-9-succinimidyl ester is a nonfluorescent acceptor molecule with a terminal NHS ester group. QSY-9-succinimidyl ester has a wide and intense quenching range from 500-600 nm that makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with fluorescent dyes at 500 nm to 600 nm. The NHS ester can be applied to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.

HY-D1916A

ATTO 594 TEA	Fluorescent Dye
ATTO 594 TEA is a highly hydrophilic fluorescent dye used to label the outermost position of the glycan moiety of glycosphingolipids to retain the biophysical and biochemical properties of native glycosphingolipids .

HY-W998662

Coumarin 343 X carboxylic acid	Fluorescent Dye
Coumarin 343 X carboxylic acid is a blue emitting fluorophore used as a laser dye. The fluorophore can serve as a FRET donor for FAM (fluorescein).

HY-125452

DiSBAC10

Fluorescent Dye

DiSBAC10 is a voltage-sensitive fluorescent probe used to study cell membrane electrical activity in FRET assays. In a resting polarized cell, DiSBAC10 resides on the outer leaflet of the membrane where it accepts photons from excited fluorescein-labeled proteins and re-emits the photons at a higher wavelength. Depolarization of the cell causes rapid translocation of DiSBAC10 to the inner leaflet of the membrane, thereby increasing the distance between fluorophores and reducing the FRET signal.

HY-D1049

DNP-X acid 6-((2,4-Dinitrophenyl)amino)hexanoic acid	Fluorescent Dye
DNP-X acid (6-((2,4-Dinitrophenyl)amino)hexanoic acid), an amine-reactive building block for developing a probe, can be recognized by anti-DNP antibodies. DNP-X acid is also an excellent amine-reactive FRET quencher paired with Trp or Tyr.

HY-D2579

Sulfo DBCO-UBQ-2	Fluorescent Dye
Sulfo DBCO-UBQ-2 is a click chemistry reagent combining a dark quencher, UBQ-2, with a polyaromatic-azo backbone, offering no native emission. UBQ-2 effectively quenches fluorescence in the 560-670 nm range, ideal for qPCR probes and FRET applications with orange to far-red dyes .

HY-D2168

AF 568 azide

Fluorescent Dye

AF 568 azide is an azide derivative of the orange fluorescent dye AF 568. AF 568 has an absorption wavelength of 590-720 nm (FRET) and an emission wavelength of 515-720 nm. AF 568 alkyne can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) via the Azide group and molecules containing Alkyne groups. Strain-promoted alkyne-azide cycloaddition (SPAAC) can also occur with molecules containing DBCO or BCN groups. To achieve specific coupling of dye labels and biomolecules .

HY-D2169

AF 568 carboxylic acid

Fluorescent Dye

AF 568 carboxylic acid is the non-reactive form of the orange fluorescent dye AF 568. AF 568 has an absorption wavelength of 590-720 nm (FRET) and an emission wavelength of 515-720 nm. AF 568 alkyne forms stable covalent bonds by reacting carboxylic acid groups with molecules bearing amino groups. Copper-catalyzed azide-alkyne cycloaddition (CuAAc) can occur with molecules containing Alkyne groups. Strain-promoted alkyne-azide cycloaddition (SPAAC) can also occur with molecules containing DBCO or BCN groups .

HY-D3310

Fluorescein-12-dGTP

Fluorescent Dye

Fluorescein-12-dGTP is a fluorescein-labeled deoxyguanosine triphosphate. Fluorescein-12-dGTP incorporates into complementary allele-specific primers at SNP sites, enabling FRET with cationic conjugated polymer PFP via DNA-PFP electrostatic interactions. Fluorescein-12-dGTP incorporates into G-allele-specific primers for homozygous G/G SNP genotypes to enable genotype discrimination. Fluorescein-12-dGTP serves as a fluorescently labeled nucleotide for homogeneous solution-based SNP and FRET genotyping assays (Ex/Em = 498/517 nm) .

HY-D3404

BODi-1

Fluorescent Dye

BODi-1 is a fluorescent modulator targeting dsDNA, which binds to dsDNA via a bis-intercalation mechanism (E_x=465 nm, E_m=490 nm). BODi-1 exhibits a fluorescence enhancement effect upon binding to nucleic acids, but its fluorescence intensity, anisotropy and average lifetime decrease at higher dye/DNA ratios. When BODi-1 binds to DNA in liposome complexes, it also shows red-shifted emission spectra, along with reduced quantum yield and average lifetime. BODi-1 does not induce significant DNA conformational changes when the dye/DNA ratio is below 0.01. BODi-1 can be used as a fluorescent probe for the characterization of liposome complexes and FRET studies at this ratio .

HY-D3275

PE-Cy5.5

Fluorescent Dye

PE-Cy5.5 is a far-red emitting tandem dye designed based on the principle of fluorescence resonance energy transfer (FRET), and is widely used in multicolor flow cytometry. PE-Cy5.5 consists of phycoerythrin (PE) as the energy donor and the cyanine dye Cy5.5 as the energy acceptor. Upon excitation by blue or green laser light, PE absorbs energy and transfers it to Cy5.5 via FRET, ultimately resulting in Cy5.5 emitting characteristic far-red fluorescence (Ex/Em = 450-500 nm/698 nm) .

HY-D3346

Dusquench1 NHS ester	Fluorescent Dye
Dusquench1 NHS ester is an amino-reactive fluorescent quencher (it does not emit light and has no emission wavelength, Ex = 480-580 nM), specially designed for labeling proteins, oligonucleotides and other biopolymers containing primary amines. It is a core reagent in the fields of qPCR probes, FRET detection and molecular beacons.

HY-D3277

PE-VF594

Fluorescent Dye

PE-VF594 is a high-brightness fluorescent dye used in flow cytometry, primarily for labeling antibodies or streptavidin to help identify specific cell subpopulations. PE-VF594 is a tandem dye composed of two covalently linked fluorescent groups: one is phycoerythrin (PE), responsible for absorbing laser energy; the other is the receptor molecule VF594. Energy is transferred from PE to VF594 via fluorescence resonance energy transfer (FRET) mechanism (Ex/Em = 450-500 nm/614 nm) .

HY-D2924

CBG-549-QSY7

Fluorescent Dye

CBG-549-QSY7 is a Dylight 549-labeled SNAP tag fluorescent probe (Ex/Em: 546 nm/580 nm), specially designed for non-washable fluorescence imaging applications. CBG-549-QSY7 employs an intramolecular FRET quenching mechanism: intramolecular fluorescence quenching occurs before binding to the SNAP tag, and upon binding, the quenching group is cleaved, releasing fluorescence. The background fluorescence of CBG-549-QSY7 is extremely low, and clear cell membrane images can be obtained after 5 minutes of incubation .

Cat. No.	Product Name	Target	Research Area

HY-P5354

FRETS-VWF73 1 Publications Verification	Fluorescent Dye	Cardiovascular Disease
FRETS-VWF73, a 73-amino-acid peptide, is a fluorogenic substrate for ADAMTS13 assay (Ex = 340 nm; Em = 450 nm). FRETS-VWF73 is a predictive tool for thrombotic thrombocytopenic purpura .

HY-P5354A

FRETS-VWF73 TFA 1 Publications Verification	Fluorescent Dye	Others
FRETS-VWF73 TFA, a 73-amino-acid peptide, is a fluorogenic substrate for ADAMTS13 assay (Ex=340 nm; Em=450 nm) .

HY-P5481

DABCYL-LPETG-EDANS	Peptides	Others
DABCYL-LPETG-EDANS is a biological active peptide. (This 5-amino acid peptide is a sortase substrate, C-terminal sorting signal. Sortase cleaves surface proteins at the LPXTG motif and catalyzes the formation of an amide bond between the carboxyl group of threonine and the amino group of cell-wall crossbridges. Sortases are a family of Gram-positive transpeptidases responsible for anchoring surface protein virulence factors to the peptidoglycan cell wall layer. Cleavage of this FRET substrate by sortase reveals the fluorescent signal, Abs/Em = 340/490 nm.)

HY-P2498A

*Cathepsin D and E FRET* Substrate acetate**	Cathepsin	Cancer
Cathepsin D and E FRET Substrate acetate is a fluorogenic substrate for cathepsins D and E and not for B, H or L. The cleavage occurs at the Phe-Phe amide bond resul. Cathepsin D and E FRET Substrate is a valuable tool for routine assays and for mechanistic studies on cathepsins E and D .

HY-P3792

Mca-Pro-Leu-Gly-Pro-D-Lys(Dnp)	Biochemical Assay Reagents	Others
Mca-Pro-Leu-Gly-Pro-D-Lys(Dnp) is a FRET substrate of Thimet oligopeptidase. Mca-Pro-Leu-Gly-Pro-D-Lys(Dnp) can be used for the determination of Thimet oligopeptidase activity .

HY-P5485

Dabcyl-QALPETGEE-Edans	Bacterial	Others
Dabcyl-QALPETGEE-Edans is a synthetic peptide substrate for fluorescence resonance energy transfer (FRET) detection of Sortase A (SrtA) enzymatic activity. Dabcyl-QALPETGEE-Edans is labeled at both ends with the fluorescent group Edans (donor) and the quencher Dabcyl (acceptor), respectively. Dabcyl-QALPETGEE-Edans's central sequence contains a conserved motif specifically recognized by SrtA. Dabcyl-QALPETGEE-Edans demonstrates high sensitivity in vitro assays, enabling precise determination of the half-maximal inhibitory concentration of SrtA .

HY-P1859

MCA-SEVNLDAEFR-K(Dnp)-RR, amide	Fluorescent Dye	Others
MCA-SEVNLDAEFR-K(Dnp)-RR, amide is a FRET-based substrate.

HY-P5419A

WAAG-3R TFA	Biochemical Assay Reagents	Others
WAAG-3R TFA is a biological active peptide. (Aggrecanases belong to the ADAMTS (A disintegrin and metalloprotease with thrombospondin motif) family of proteases. Aggrecanases cleave aggrecan, the major structural component of cartilage. Aggrecanase-1 (ADAMTS-4) is a major aggrecanase in human osteoarthritic cartilage. This FRET peptide was used in an ADAMTS-4 (Aggrecanase-1) and ADAMTS-5 (Aggrecanase-2) assay . (Ex/Em = 340/420 nm)

HY-P2498

*Cathepsin D and E FRET* Substrate**	Cathepsin	Others
Cathepsin D and E FRET Substrate is a fluorogenic substrate for cathepsins D and E and not for B, H or L. The cleavage occurs at the Phe-Phe amide bond resul. Cathepsin D and E FRET Substrate is a valuable tool for routine assays and for mechanistic studies on cathepsins E and D .

HY-P11302

DABCYL-RAGGYIFS-(E-EDANS)-NH2	CHIKV Virus Protease	Infection
DABCYL-RAGGYIFS-(E-EDANS)-NH2, an octapeptide, is a FRET-based substrate peptide of CHIKV nsP2protease with highly sensitivity. DABCYL-RAGGYIFS-(E-EDANS)-NH2 can be cleaved by the active CHIKV nsP2protease, separating fluorophore and quencher and resulting in an increase of fluorescence signal. DABCYL-RAGGYIFS-(E-EDANS)-NH2 can used for the determination of CHIKV nsP2protease activity .

HY-P10344

MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2	Fluorescent Dye	Infection
MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 is a fluorescent substrate used for fluorescence resonance energy transfer (FRET) protease assays. This substrate is employed to measure the activity of SARS-CoV-2 3CLpro .

HY-P4787A

Ac-Glu-Asp(EDANS)-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Gly-Lys(DABCYL)-Glu-NH2 acetate	Cathepsin	Others
Ac-Glu-Asp(EDANS)-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Gly-Lys(DABCYL)-Glu-NH2 acetate is a Cathepsin D substrate, that can be used for cathepsin D FRET assay .

HY-P4787

Ac-Glu-Asp(EDANS)-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Gly-Lys(DABCYL)-Glu-NH2	Cathepsin	Others
Ac-Glu-Asp(EDANS)-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Gly-Lys(DABCYL)-Glu-NH2 is a cathepsin D substrate, that can be used for cathepsin D FRET assay .

HY-P2492

*Renin FRET* Substrate I**	Angiotensin Receptor	Metabolic Disease
Renin FRET Substrate I is a substrate of human renin. Renin FRET Substrate I is designed to incorporate the renin cleavage site that occurs in the N-terminal peptide of human angiotensinogen .

HY-P5377

Abz-HPGGPQ-EDDnp Cathepsin K substrate	Ser/Thr Protease	Others
Abz-HPGGPQ-EDDnp (Cathepsin K substrate) is a biological active peptide. (Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin K is the lysosomal cysteine protease involved in bone remodeling and resorption. It has potential as a drug target in autoimmune diseases and osteoporosis.This FRET peptide can be used to monitor selectively cathepsin K activity in physiological fluids and cell lysates. Abz-HPGGPQ-EDDnp [where Abz represents o-aminobenzoic acid and EDDnp represents N -(2, 4-dinitrophenyl)-ethylenediamine], a substrate initially developed for trypanosomal enzymes, is efficiently cleaved at the Gly-Gly bond by cathepsin K. This peptide is resistant to hydrolysis by cathepsins B, F, H, L, S and V, Ex/Em=340 nm/420 nm.)

HY-P5970

DABCYL-TNF-α-EDANS (-4 to +6) (human) TACE Substrate	Peptides	Cancer
DABCYL-TNF-α-EDANS (-4 to +6) (human) is a FRET peptide substrate of tumor necrosis factor convertase (TACE) .

HY-P10196

DABCYL-SEVNLDAEF-EDANS	Peptides	Others
DABCYL-SEVNLDAEF-EDANS is a substrate of FRET. DABCYL-SEVNLDAEF-EDANS can be used in the BACE activity assay .

HY-P5323

Dabcyl-AGHDAHASET-Edans	Bacterial	Others
Dabcyl-AGHDAHASET-Edans is a biological active peptide. (This is a type I signal peptidase (SPase1) substrate peptide labeled with EDANS/ DABCYL FRET pair, and contains a crucial cleavage site derived from the C-terminal region of the Staphylococcus epidermidis pre-SceD protein. Abs/Em = 340/490 nm.)

HY-P5344

Dabcyl-LNKRLLHETQ-Edans Fluorigenic PEXEL peptide	Parasite	Others
Dabcyl-LNKRLLHETQ-Edans (Fluorigenic PEXEL peptide) is a biological active peptide. (This FRET substrate peptide for Plasmepsin V (PMV) is derived from the conserved Plasmodium Export Element (PEXEL) motif of Histidine-Rich Protein II (HRPII). PMV is an ER aspartic protease that recognizes and cleaves the RXL sequence within the PEXEL motif of proteins exported by human malaria parasite Plasmodium falciparum, allowing them to translocate into host erythrocytes.)

HY-P5419

WAAG-3R	Peptides	Others
WAAG-3R is a biological active peptide. (Aggrecanases belong to the ADAMTS (A disintegrin and metalloprotease with thrombospondin motif) family of proteases. Aggrecanases cleave aggrecan, the major structural component of cartilage. Aggrecanase-1 (ADAMTS-4) is a major aggrecanase in human osteoarthritic cartilage. This FRET peptide was used in an ADAMTS-4 (Aggrecanase-1) and ADAMTS-5 (Aggrecanase-2) assay . (Ex/Em = 340/420 nm)

HY-P5528

2Abz-SLGRKIQIK(Dnp)-NH2	Peptides	Others
2Abz-SLGRKIQIK(Dnp)-NH2 is a biological active peptide. (This peptide is a second complement component (C2), the physiological substrate for the proenzyme Cls, first complement component. The complement system is a central component of host defense but can also contribute to the inflammation seen in pathological conditions. The C1s protease of the C1 complex initiates the host defense pathway. This peptide employs 2Abz/Dnp FRET pair for quantitation of complement enzyme activity.)

HY-P4919

Mca-SEVNLDAEFK(Dnp)	Beta-secretase	Others
Mca-SEVNLDAEFK(Dnp) is a Beta-secretase 1 (BACE-1) peptide FRET substrate, containing the 'Swedish' Lys-Met/Asn-Leu mutation of the amyloid precursor protein (APP) β-secretase cleavage site. Cleavage at -Leu-Asp- of Mca-SEVNLDAEFK(Dnp) liberates the highly fluorescent 7-methoxycoumarin (Mca) fragment from the proximity quenching effect of the 2,4-dinitrophenyl (Dnp) internal quencher resulting in a large and easily detectable increase in fluorescence intensity.

HY-P5415

DABCYL-GABA-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS	HIV	Others
DABCYL-GABA-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS is a biological active peptide. (DABCYL-GABA-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS is also called HIV protease substrate I in some literature. It is widely used for the continuous assay for HIV protease activity. The 11-K_d protease (PR) encoded by the human immunodeficiency virus 1 (HIV-1) is essential for the correct processing of viral polyproteins and the maturation of infectious virus, and is therefore a target for the design of selective acquired immunodeficiency syndrome (AIDS) therapeutics. The FRET-based fluorogenic substrate is derived from a natural processing site for HIV-1 PR. Incubation of recombinant HIV-1 PR with the fluorogenic substrate resulted in specific cleavage at the Tyr-Pro bond and a time-dependent increase in fluorescence intensity that is linearly related to the extent of substrate hydrolysis. The fluorescence quantum yields of the HIV-1 PR substrate in the FRET assay increased by 40.0- and 34.4-fold, respectively, per mole of substrate cleaved. Because of its simplicity and precision in the determination of reaction rates required for kinetic analysis, this substrate offers many advantages over the commonly used HPLC or electrophoresis-based assays for peptide substrate hydrolysis by retroviral PRs. Abs/Em = 340nm/490nm.)

HY-P1859A

MCA-SEVNLDAEFR-K(Dnp)-RR, amide acetate	Biochemical Assay Reagents	Others
MCA-SEVNLDAEFR-K(Dnp)-RR, amide (acetate) is synthesized from MCA-SEVNLDAEFR-K(Dnp)-RR, amide (HY-P1859) by losing the H of the -OH group of acetic acid. MCA-SEVNLDAEFR-K(Dnp)-RR, amide is a FRET-based substrate.

HY-P11800A

GPLGVRGC	Exosomes MMP	Inflammation/Immunology
GPLGVRGC is a cysteine-tagged variant of GPLGVRG (HY-P11800). GPLGVRGC is hydrolyzable by MMP13. GPLGVRGC mediates the disassembly of micelle-exosome systems, enhances chondrocyte endocytosis, and promotes responsive system uptake. GPLGVRGC confers targeted delivery and responsive release properties to micelle-exosome systems. GPLGVRGC is applicable to the research of osteoarthritis .

Cat. No.	Product Name	Target	Research Area	Image

HY-P99900

Sevirumab MSL-109	CMV	Infection
Sevirumab (MSL-109) is a human IgG1 neutralizing monoclonal antibody against cytomegalovirus (CMV). Sevirumab recognizes CMV gH complexes and inhibits CMV replication with an EC₅₀ of 0.3 μg/mL .

Cat. No.	Product Name	Chemical Structure

HY-W027544S

MCA-d3
MCA-d₃ (7-Methoxycoumarin-4-acetic acid₃) is the deuterium labeled MCA (HY-W027544) . MCA is a Coumarin (HY-N0709) derivative. MCA quantitates platelet-activating factor (PAF) by high-performance liquid chromatography with fluorescent detection. MCA can modify FRET peptide substrates for analyzing protease activities .

Cat. No.	Product Name		Classification

HY-W800703

Alexa Fluor 594 azide		Azide
Alexa Fluor 594 azide is an azide-modified fluorescent dye and also a *FRET* acceptor reactant. Owing to its negligible direct absorption under 488 nm excitation light, excellent photostability, and the ability of its azide group to participate in the CuAAC reaction, Alexa Fluor 594 azide can be paired with a donor dye for single-molecule imaging via TIRFM, thereby enabling the visual monitoring of CuNP-catalyzed click reactions .

HY-D2167

AF 568 alkyne		Alkynes
AF 568 alkyne is a derivative of the orange fluorescent dye AF 568. AF 568 has an absorption wavelength of 590-720 nm (FRET) and an emission wavelength of 515-720 nm. AF 568 alkyne can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) via the Alkyne group and molecules containing Azide groups. To achieve specific coupling of dye labels and biomolecules .

HY-D2579

Sulfo DBCO-UBQ-2		DBCO
Sulfo DBCO-UBQ-2 is a click chemistry reagent combining a dark quencher, UBQ-2, with a polyaromatic-azo backbone, offering no native emission. UBQ-2 effectively quenches fluorescence in the 560-670 nm range, ideal for qPCR probes and FRET applications with orange to far-red dyes .

Cat. No.	Product Name		Classification

HY-160276

DOPE-PEG5000-Fluor 555		Pegylated Lipids Fluorescent Lipids
DOPE-PEG5000-Fluor 555 is consist of a DOPE phospholipid which is an unsaturated phospholipid and a Fluor 555 dye which is a bright orange cyanine dye that can be used in fluorescence microscopy, FRET and other in vivo imaging techniques.

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