1. GPCR/G Protein
    Neuronal Signaling
  2. Dopamine Receptor
  3. SKF83822 hydrobromide

SKF83822 hydrobromide 

Cat. No.: HY-103411
Handling Instructions

SKF83822 hydrobromide is a potent dopamine D1 receptor agonist. SKF83822 hydrobromide activates Gs/olf/adenylyl cyclase (AC)-coupled D1 receptors, but not phospholipase C (PLC)-coupled D1-like receptors.

For research use only. We do not sell to patients.

SKF83822 hydrobromide Chemical Structure

SKF83822 hydrobromide Chemical Structure

CAS No. : 74115-10-9

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Description

SKF83822 hydrobromide is a potent dopamine D1 receptor agonist. SKF83822 hydrobromide activates Gs/olf/adenylyl cyclase (AC)-coupled D1 receptors, but not phospholipase C (PLC)-coupled D1-like receptors[1].

IC50 & Target[1]

D1 Receptor

 

In Vitro

SKF83822 (1 μM) increases DARPP-32 phosphorylation in Neostriatal slices. Treatment with SKF83822 for 5 min stimulates DARPP-32 Thr34 phosphorylation maximally at a concentration of 100 μM with a half maximal effect at ∼1 μM. SKF83822 (1 μM) does not affect the phosphorylation of DARPP-32 at Thr75, Ser97 or Ser130[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: Neostriatal slices
Concentration: 1 μM
Incubation Time: 0, 0.5, 2, 5, and 10 minutes
Result: Treatment with 1 μM increased the level of phospho-Thr34 DARPP-32, maximally by ∼6-fold within 2 min of incubation.
In Vivo

SKF83822 activates dopamine D1 receptors coupled to Gαs/olf and downstream cyclase activity. SKF83822 produces a locomotor response in both rodent and non-human primate models without affecting stereotypy, intense grooming, or dyskinesia. An acute injection of SKF83822 (0.4 mg/kg; i.p.) induced a greater than threefold increase in locomotor activity relative to the baseline period for each genotype[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Gαq knockout mice[1]
Dosage: 0.4 mg/kg
Administration:
Result: There was a significant increase in locomotor activity in each genotype.
Molecular Weight

424.76

Formula

C₂₀H₂₃BrClNO₂

CAS No.
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Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

References
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SKF83822 hydrobromide
Cat. No.:
HY-103411
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