SM-360320
Based on 1 Customer Validation
SM-360320 (CL-087) is a potent, orally active TLR7 agonist. SM-360320 is a immuno-modulator and exerts an antitumor effect. SM-360320 can act in synergy with DNA vaccines leading to an enhanced Th1 antibody response. SM-360320 can inhibit HCV replication in hepatocytes via a type I IFN-independent mechanism in addition to its IFN-mediated activity.
For research use only. We do not sell to patients.
- Purity: 99.41%
- CAS No.: 226907-52-4
- Formula: C15H17N5O3
- Molecular Weight:315.33
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | EC50 |
1.17 μM
Compound: SM360320
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Agonist activity at human TLR7 expressed in HEK293 cells assessed as induction of NFKappaB activity after 24 hrs by SEAP reporter gene assay
Agonist activity at human TLR7 expressed in HEK293 cells assessed as induction of NFKappaB activity after 24 hrs by SEAP reporter gene assay
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[PMID: 32001135] |
| HEK293 | EC50 |
399.4 nM
Compound: 3
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Agonist activity at human TLR7 expressed in HEK293 cells coexpressing pNiFty2-SEAP reporter by reporter gene assay
Agonist activity at human TLR7 expressed in HEK293 cells coexpressing pNiFty2-SEAP reporter by reporter gene assay
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[PMID: 20232824] |
| Huh-7 | EC50 |
30 nM
Compound: BHMA
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Antiviral activity against HCV infected in human Huh7 cells with persistent PFK1389-Luc-Ubi-Neo-NS3-3'/ET replicon after 48 hrs by luminescence based assay
Antiviral activity against HCV infected in human Huh7 cells with persistent PFK1389-Luc-Ubi-Neo-NS3-3'/ET replicon after 48 hrs by luminescence based assay
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[PMID: 20028817] |
| PBMC | EC50 |
0.14 μM
Compound: 4, SM360320
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Induction of IFNalpha production in human PBMC
Induction of IFNalpha production in human PBMC
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[PMID: 16784848] |
Chemical Information
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CAS No. 226907-52-4
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Appearance Solid
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Molecular Weight 315.33
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Formula C15H17N5O3
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Color White to off-white
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SMILES
COCCOC1=NC(N)=C2C(N(C(N2)=O)CC3=CC=CC=C3)=N1
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Synonyms
CL-087
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 125 mg/mL (396.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Purity & Documentation
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Data Sheet (271 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Dharmapuri S, et al. An oral TLR7 agonist is a potent adjuvant of DNA vaccination in transgenic mouse tumor models. Cancer Gene Ther. 2009;16(5):462-472. [Content Brief]
[2]. Lee J, et al. Activation of anti-hepatitis C virus responses via Toll-like receptor 7. Proc Natl Acad Sci U S A. 2006;103(6):1828-1833. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1713 mL | 15.8564 mL | 31.7128 mL | 79.2820 mL |
| 5 mM | 0.6343 mL | 3.1713 mL | 6.3426 mL | 15.8564 mL | |
| 10 mM | 0.3171 mL | 1.5856 mL | 3.1713 mL | 7.9282 mL | |
| 15 mM | 0.2114 mL | 1.0571 mL | 2.1142 mL | 5.2855 mL | |
| 20 mM | 0.1586 mL | 0.7928 mL | 1.5856 mL | 3.9641 mL | |
| 25 mM | 0.1269 mL | 0.6343 mL | 1.2685 mL | 3.1713 mL | |
| 30 mM | 0.1057 mL | 0.5285 mL | 1.0571 mL | 2.6427 mL | |
| 40 mM | 0.0793 mL | 0.3964 mL | 0.7928 mL | 1.9821 mL | |
| 50 mM | 0.0634 mL | 0.3171 mL | 0.6343 mL | 1.5856 mL | |
| 60 mM | 0.0529 mL | 0.2643 mL | 0.5285 mL | 1.3214 mL | |
| 80 mM | 0.0396 mL | 0.1982 mL | 0.3964 mL | 0.9910 mL | |
| 100 mM | 0.0317 mL | 0.1586 mL | 0.3171 mL | 0.7928 mL |
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