SPG-178
SPG-178 is a synthetic neutral Self-assembling peptide. In neutral aqueous solution, SPG-178 spontaneously assembles into an antiparallel β-sheet nanoscale three-dimensional hydrogel (SPG-178-Gel) through electrostatic and hydrophobic interactions, and biologically mimics the natural extracellular matrix (ECM) to provide a microenvironment that promotes cell proliferation and angiogenesis.
For research use only. We do not sell to patients.
- CAS No.: 1245010-44-9
- Formula: C73H134N26O18
- Molecular Weight:1664.01
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
SPG-178 promotes neurite outgrowth in in vitro cultured motor neurons, accompanied by increased expression of NGF, BDNF, NT-4, TrkA, and TrkB[2].
1.5% SPG-178 (0.8-1.5%; up to 72 h) significantly increases the viability/proliferation of rPDL cells at 72 h compared to 0.8% SPG-178 and untreated controls[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:rPDL cells
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Concentration:0.8% (SPG-178); 1.5% (SPG-178)
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Incubation Time:72 h
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Result:Demonstrated a rapid increase in viability/proliferation at 72 h in the 1.5% SPG-178 group.
Showed only a moderate increase in the 0.8% SPG-178 or the control group.
At 72 h, cell viability/proliferation of the 1.5% SPG-178 group was significantly greater than that of the control group or 0.8% SPG-178 group.
In Vivo
SPG-178 (1.5% 2 mL; topical application; single administration; 3 days) prevents pancreatic fistula and significantly reduces ascites amylase levels in an SD rat pancreatic fistula model[6].
SPG-178 (0.8% 200 μL combined with 0.2 g bone fragments; local implantation; single administration; 56 days) promotes new bone formation and bone fusion in the Kbl:JW rabbit posterolateral lumbar fusion model[7].
SPG-178 (1.0%-1.5% 100 μL; topical application; single administration; 10 min-7 days) exhibits significant topical hemostatic efficacy by shortening bleeding time in a Wistar rat model of liver incision hemorrhage[8].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Wistar (Male, 10 wk old, 250-300 g, periodontal defect model)[3]
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Dosage:50 µl of 1.5% SPG-178
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Administration:local implantation; single application; 2, 4 weeks
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Result:Promoted the filling of connective tissue in the periodontal defect area and accelerated early cell proliferation.
Accelerated new bone formation, significantly increasing the bone volume fraction.
Limited epithelial down-growth, facilitating the robust regeneration of periodontal ligaments.
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Animal Model:Sprague-Dawley (SD) (male, eight-week-old, 272-364 g, pancreatic fistula model)[6]
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Dosage:2 mL of SPG-178 (1.5 w/w%)
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Administration:topical application; single dose; 3 days
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Result:Significantly reduced ascitic amylase levels at 3 days post-operation.
Tightly covered the surface of the pancreatic stump, reducing the invasion of inflammatory cells and the destruction of pancreatic acinar cells.
Formed a fibrous tissue or mesothelium covering on its surface, effectively blocking the leakage of pancreatic juice.
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Animal Model:Kbl:JW (male, 20 weeks old, 3-3.5 kg)[7]
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Dosage:200 μl of 0.8% hydrogel (mixed with 0.2 g of bone chips)
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Administration:local implantation in the fusion area; single dose; treatment duration of 56 days
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Result:Induced clear new bone formation.
Promoted a high amount of calcified new bone attached to the lamina, particularly at the margin of the implant.
- 3D Hydrogel Synthetic Scaffold Culture
- Collagen: Sirius Red Staining
- Human pluripotent stem cell endothelial-cell differentiation
- Vascular/Branching Fractal Analysis
- BrdU Incorporation Assay
- Somatic Cell Culture
- ECM-Embedded Organoid (Matrigel/Dome) Culture
- CFSE Dye Dilution Proliferation Assay
- Dye-dilution cell tracking and proliferation staining
- CCK-8/WST-8 Cell Proliferation Assay
- Protocol for Cell Cycle
- Cell Counting-Based Growth Curve Assay
- Colony Formation (Clonogenic) Assay
- Matrigel Transwell/Boyden Chamber Invasion Assay
- Cell Viability Determination by MTT Colorimetric Assay
- Fibrosis/Collagen Morphometry
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Ki-67 Immunostaining Proliferation Assay
- PCNA Immunodetection Proliferation Assay
- Protocol for Cell Counting and Cell Density Analysis
- Endothelial Tube Formation Assay
- Matrigel/ECM Transwell Invasion Assay
- MTT Cell Proliferation Assay
- Endothelial Cell Migration/Angiogenic Sprouting Assay
Chemical Information
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CAS No. 1245010-44-9
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Molecular Weight 1664.01
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Formula C73H134N26O18
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Sequence
Ac-Arg-Leu-Asp-Leu-Arg-Leu-Ala-Leu-Arg-Leu-Asp-Leu-Arg-NH2
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Sequence Shortening
Ac-RLDLRLALRLDLR-NH2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
SPG-178 Related Classifications
HY-P12358 Related Classifications
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Keywords
- SPG-178
- 1245010-44-9
- SPG178
- SPG 178
- Biochemical Assay Reagents
- neurite outgrowth
- periodontal ligament cell proliferation
- osteoblastic differentiation
- rPDL cells
- DPSC proliferation
- osteoporosis
- bone regeneration
- p38 MAPK-dependent BMP-2 production
- rabbit tibial intramedullary defects
- C2C12 cells
- Inhibitor
- inhibitor
- inhibit