Trijuganone C
Trijuganone C is a tanshinone-type diterpenoid compound. Trijuganone C can be isolated from the roots of Salvia miltiorrhiza Bunge. Trijuganone C induces chromatin condensation, DNA fragmentation, activation of Caspase-3, -8 and -9, as well as cleavage of PARP. Trijuganone C activates Bid and Bax, leading to loss of mitochondrial membrane potential and inducing the release of cytochrome c from mitochondria into the cytosol. Trijuganone C exerts antiproliferative effects through Apoptosis induction mediated by Mitochondrial dysfunction and Caspase activation. Trijuganone C exhibits significant antiproliferative activity against leukemia cells and colon cancer cells.
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- CAS No.: 135247-94-8
- Formula: C20H20O5
- Molecular Weight:340.37
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
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Biological Activity
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Caspase-8 |
Caspase-9 |
Caspase-3 |
Bax |
Trijuganone C (6.25-25 μM; 24 h) potently inhibits the proliferation of HL-60, Jurkat and U937 leukemia cell lines, with IC50 values of 6.1, 8.9 and 13.4 μM respectively after 24 h of treatment[1].
Trijuganone C (24 h) potently inhibits the proliferation of colon cancer cell lines DLD-1, COLO 205 and Caco-2 (IC50 6.1-8.4 μM), exerts moderate inhibitory effects on other cancer cell lines and some normal cell lines, and shows no activity against HepG2, WRL 68 and NB1RGB cells after 24 h of treatment[1].
Trijuganone C (12.5 μM; 24 h) induces chromatin condensation, a marker of apoptosis, in leukemia HL-60 cells[1].
Trijuganone C (3.125-25 μM; 24 h) induces dose-dependent apoptotic DNA fragmentation in leukemia cell line HL-60[1].
Trijuganone C (12.5 μM; 3-12 h) induces time-dependent activation of PARP, caspase-3, caspase-8 and caspase-9 in HL-60 leukemia cells[1].
Trijuganone C (12.5 μM; 3-12 h) induces the release of mitochondrial cytochrome c into the cytosol in a time-dependent manner in HL-60 leukemia cells[1].
Trijuganone C (12.5 μM; 3-12 h) induces time-dependent activation (cleavage into t-Bid) of the pro-apoptotic protein Bid, and promotes the translocation of Bax from the cytoplasm to mitochondria, with no effect on the anti-apoptotic proteins Bcl-2 or Bcl-xL[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HL-60, Jurkat, U937
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Concentration:6.25 μM, 12.5 μM, 25 μM
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Incubation Time:24 h
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Result:Significantly suppressed the growth of all three leukemia cell lines.
Exhibited IC50 values of 6.1 μM for HL-60, 8.9 μM for Jurkat, and 13.4 μM for U937 cells.
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Cell Line:DLD-1, COLO 205, Caco-2, HCT-15, PC-3, LNCap FGC, MCF-7, HepG2, MRC-7, WRL 68, NB1RGB
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Concentration:<25 μM
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Incubation Time:24 h
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Result:Strongly suppressed growth of colon cancer cell lines DLD-1 (IC50 = 6.1 μM), COLO 205 (IC50 = 7.2 μM), and Caco-2 (IC50 = 8.4 μM).
Weakly suppressed growth of colon cancer HCT-15 (IC50 = 13.2 μM), prostate cancer PC-3 (IC50 = 11.2 μM) and LNCap FGC (IC50 = 13.7 μM), breast cancer MCF-7 (IC50 = 16.7 μM), and normal lung MRC-7 (IC50 = 17.1 μM).
Had no effect on liver cancer HepG2, normal liver WRL 68, and normal fibroblast NB1RGB (IC50 >25 μM for all three).
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Cell Line:HL-60
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Concentration:12.5 μM
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Incubation Time:3 h, 6 h, 9 h, 12 h
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Result:Induced time-dependent cleavage of full-length PARP to its cleaved form.
Induced time-dependent activation of caspase-3, caspase-8, and caspase-9 (conversion from pro-caspase to active form).\nInduced time-dependent accumulation of cytochrome c in the cytosolic fraction.\nInduced time-dependent cleavage of Bid to its active truncated form (t-Bid), but had no effect on antiapoptotic Bcl-2 and Bcl-xL levels.
Promoted time-dependent translocation of Bax from the cytosol to the mitochondrial fraction.
Chemical Information
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CAS No. 135247-94-8
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Molecular Weight 340.37
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Formula C20H20O5
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SMILES
O=C([C@@]1(C)CCCC2=C1C=CC(C3=C4[C@@H](C)CO3)=C2C(C4=O)=O)OC
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Trijuganone C Related Classifications
HY-N8617 Related Classifications
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