1. Apoptosis
  2. Apoptosis
  3. Ucf-101

Ucf-101 

Cat. No.: HY-125959 Purity: 98.33%
Handling Instructions

Ucf-101 is a selctive and competitive inhibitor of pro-apoptotic protease Omi/HtrA2, with an IC50 of 9.5 μM for His-Omi. Ucf-101 exhibits very little activity against various other serine proteases (IC50>200 μM). Ucf-101 has a natural red fluorescence at 543 nm that is used to monitor its ability to enter mammalian cells. Ucf-101 has a significant cardioprotective effect against MI/R injury and also has certain neuroprotective effect.

For research use only. We do not sell to patients.

Ucf-101 Chemical Structure

Ucf-101 Chemical Structure

CAS No. : 313649-08-0

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10 mM * 1 mL in DMSO USD 110 In-stock
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10 mg USD 170 In-stock
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25 mg USD 350 In-stock
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50 mg USD 550 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

Ucf-101 is a selctive and competitive inhibitor of pro-apoptotic protease Omi/HtrA2, with an IC50 of 9.5 μM for His-Omi. Ucf-101 exhibits very little activity against various other serine proteases (IC50>200 μM). Ucf-101 has a natural red fluorescence at 543 nm that is used to monitor its ability to enter mammalian cells. Ucf-101 has a significant cardioprotective effect against MI/R injury and also has certain neuroprotective effect[1][2][3].

IC50 & Target

IC50: 9.5 μM (His-Omi)[1]

In Vitro

Ucf-101 (20-100 μM; 30 min) inhibits the proteolytic activity of MBP-Omi-(134-458)[1].
Ucf-101 (10-100 μM; pretreated for 10 min) inhibits His-Omi-(134-458) activity in a concentration-dependent manner when assayed with His-Omi-(134-458) and β-casein[1].
Ucf-101 (1-25 μM; 36 h) inhibits Omi-induced caspase-independent apoptosis of mouse embryo caspase-9 (-/-) null fibroblasts[1].
Ucf-101 (1-20 μM; pretreated for 1 h) inhibits the 6-OHDA-induced apoptosis of Parkinson’s disease (PD)-PC12 cells at the low concentration (2.5 μM), and increases the apoptosis rate at the high concentration (≥10 μM)[3].
Ucf-101 (2.5 μM; pretreated for 1 h) downregulates the expression of Glucose-regulated protein 78 (Bip/Grp78) and C/EBP homologous protein (CHOP) in PD- PC12 cells[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Ucf-101 (0.6-1.8 μmol/kg; a single i.p.) reduces postischemic myocardial apoptosis and myocardial infarct size in mice[2].
Ucf-101 (1.5 μmol/kg; a single i.p.) improves the APO-induced rotational behavior, increases the TH-positive cells and reverses the reduction of DA neurons in the PD rats[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Adult male mice (20-25 g) with myocardial ischemia/reperfusion (MI/R) injury[1]
Dosage: 0.6, 1.5, 1.8 μmol/kg
Administration: I.p. 10 minutes before reperfusion
Result: Reduced terminal dUTP nick end-labeling staining, incidence of DNA ladder fragmentation, and infarct size.
Molecular Weight

495.51

Formula

C₂₇H₁₇N₃O₅S

CAS No.

313649-08-0

SMILES

O=C(/C(C(N1C2=CC=CC=C2)=O)=C/C3=CC=C(C4=C([N+]([O-])=O)C=CC=C4)O3)N(C5=CC=CC=C5)C1=S

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 12.5 mg/mL (25.23 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0181 mL 10.0906 mL 20.1812 mL
5 mM 0.4036 mL 2.0181 mL 4.0362 mL
10 mM 0.2018 mL 1.0091 mL 2.0181 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: 1.67 mg/mL (3.37 mM); Suspended solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 1.79 mg/mL (3.61 mM); Suspended solution; Need ultrasonic

*All of the co-solvents are provided by MCE.
References
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Keywords:

Ucf-101Ucf101Ucf 101Apoptosispro-apoptoticproteaseOmiHtrA2His-Omiredfluorescence543cardioprotectiveMI/RneuroprotectiveInhibitorinhibitorinhibit

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