N4Py

N4Py forms complexes with Fe²⁺, Fe³⁺, Cu²⁺, Mn²⁺, and Zn²⁺ that differ in spectral and electrochemical properties, and Fe²⁺-N4Py exhibits characteristic metal-to-ligand charge transfer absorption^[1].
N4Py can form Fe (II)-N4Py and Fe (III)-N4Py, both of which efficiently cleave supercoiled pUC18 plasmid DNA under cell-free conditions^[1].
Mn (II)-N4Py, Fe (II)-N4Py and Fe (III)-N4Py formed by N4Py significantly reduce the metabolic activity of A2780, SKOV3 and OSE-C2 cells, with IC₅₀ values of 5-10 μM^[1].
Mn (II)-N4Py, Fe (II)-N4Py and Fe (III)-N4Py induce significant late apoptosis/necrotic cell death in A2780, SKOV3 and OSE-C2 cells, among which free N4Py exhibits the strongest cytotoxicity, while Cu (II)-N4Py and Zn (II)-N4Py show minimal cytotoxicity^[1].
Mn (II)-N4Py, Fe (II)-N4Py, Fe (III)-N4Py, and Cu (II)-N4Py induce hROS production in A2780 cells, while Zn (II)-N4Py has no such effect; NAC abrogates hROS production induced by all these substances except Cu (II)-N4Py, and instead enhances hROS production induced by Cu (II)-N4Py^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.