Cat. No.: HY-W008947

SEW2871

Name: SEW2871
Brand: MedChemExpress
SKU: HY-W008947
Price: 62.00 USD
Availability: InStock
Rating: 5 (11 reviews)

11 Cited Publications

Customer Review

Based on 11 publication(s) in Google Scholar

SEW2871 is an orally active, potent, highly selective S1P1 (sphingosine-1-phosphate type 1 receptor) agonist, with an EC₅₀ of 13.8 nM. SEW2871 activates ERK, Akt, and Rac signaling pathways and induces S1P1 internalization and recycling. SEW2871 reduces lymphocyte numbers in blood. SEW2871 can be used for the research of diabetes, Alzheimer’s disease, liver fibrosis, and inflammatory responses.

For research use only. We do not sell to patients.

Purity : 99.88%
CAS No.: 256414-75-2
Formula: C₂₀H₁₀F₆N₂OS
Molecular Weight:440.36

Storage:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

SEW2871 Chemical Structure

CAS No.: 256414-75-2

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (11)
Solvent & Solubility
- In Vitro
- In Vivo
Protocols (5)
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

S1PR1

13.8 nM (EC₅₀)

All LPL Receptor Isoforms :

All ERK Isoforms :

All Akt Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	>10000 nM Compound: 10	Displacement of [33P]sphingosine 1 phosphate from human S1P3 receptor expressed in CHO cells Displacement of [33P]sphingosine 1 phosphate from human S1P3 receptor expressed in CHO cells	[PMID: 15615513]
CHO	IC₅₀	>10000 nM Compound: 10	Displacement of [33P]sphingosine 1 phosphate from human S1P4 receptor expressed in CHO cells Displacement of [33P]sphingosine 1 phosphate from human S1P4 receptor expressed in CHO cells	[PMID: 15615513]
CHO	IC₅₀	37 nM Compound: 10	Displacement of [33P]sphingosine 1 phosphate from human S1P1 receptor expressed in CHO cells Displacement of [33P]sphingosine 1 phosphate from human S1P1 receptor expressed in CHO cells	[PMID: 15615513]
CHO	IC₅₀	4600 nM Compound: 10	Displacement of [33P]sphingosine 1 phosphate from human S1P5 receptor expressed in CHO cells Displacement of [33P]sphingosine 1 phosphate from human S1P5 receptor expressed in CHO cells	[PMID: 15615513]
CHO	IC₅₀	>10000 nM Compound: 10	Displacement of [33P]sphingosine 1 phosphate from human S1P2 receptor expressed in CHO cells Displacement of [33P]sphingosine 1 phosphate from human S1P2 receptor expressed in CHO cells	[PMID: 15615513]
CHO	IC₅₀	>10 nM Compound: SEW-2871	Displacement of [33P]S1P from human S1P3R expressed in CHO cell membranes Displacement of [33P]S1P from human S1P3R expressed in CHO cell membranes	[PMID: 24900286]
CHO	IC₅₀	37 nM Compound: SEW-2871	Displacement of [33P]S1P from human S1P1R expressed in CHO cell membranes Displacement of [33P]S1P from human S1P1R expressed in CHO cell membranes	[PMID: 24900286]
CHO-K1	EC₅₀	>25 μM Compound: 3, SEW2871	Agonist activity at human S1P3 receptor expressed in CHO-K1 cells co-expressing Gq/i5 G-protein assessed as calcium mobilization by FLIPR assay Agonist activity at human S1P3 receptor expressed in CHO-K1 cells co-expressing Gq/i5 G-protein assessed as calcium mobilization by FLIPR assay	[PMID: 22104144]
U2OS	EC₅₀	0.27 μM Compound: 3, SEW2871	Agonist activity at human S1P1 receptor expressed in human U2OS cells co-expressing eGFP assessed as receptor internalization into cytoplasm using Hoechst dye staining Agonist activity at human S1P1 receptor expressed in human U2OS cells co-expressing eGFP assessed as receptor internalization into cytoplasm using Hoechst dye staining	[PMID: 22104144]

In Vitro

SEW2871 exerts a powerful migratory effect by increasing smooth muscle α-actin, procollagen αI and αIII, and total hydroxyproline contents in LX-2 cells (a human hepatic stellate cell line)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

In Vivo

SEW2871 (20 mg/kg, Gavage, once daily for 2 weeks) ameliorates established experimental colitis in IL-10^–/– mice^[3].
? SEW2871 (0.5 mg/kg, IP, daily for 2 weeks) inhibited β amyloid (Aβ_1-42)-induced spatial memory impairment and hippocampal neuronal loss in a rat model of Alzheimer’s disease^[2].
? SEW2871 (0-0.3 mg/kg, IV) attenuates LPS-induced acute inflammatory lung injury, produces dose-dependent alveolar and vascular barrier protection in C57Bl/6 mice^[2].
? SEW2871 is effective in protecting kidneys against ischaemia-reperfusion injury by reducing CD4⁺ T cell infiltration in mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

IL-10^–/– (interleukin (IL)-10 gene-deficient) mice, a murine model of Crohn's disease (CD)^[3]
Dosage:

20 mg/kg
Administration:

Gavage, once daily for 2 weeks
Result:

Ameliorated established colitis in IL-10^–/– mice, associated with a reduction of serum amyloid A concentration, a decreased colon MPO concentration, a depletion of the peripheral CD4⁺CD45⁺ T cells and a reduction of the homing of T cells into colon LP. Suppressed typical cytokines of T helper type 1 (Th1) and Th17 cells and p-STAT-3 expression, and significantly decreased TNF-α, IFN-γ, IL-1β and IL-17A mRNA levels.

Chemical Information

CAS No. 256414-75-2
Appearance Solid
Molecular Weight 440.36
Formula C₂₀H₁₀F₆N₂OS
Color White to light yellow
SMILES

FC(C1=CC(C2=NOC(C3=CC(C4=CC=CC=C4)=C(C(F)(F)F)S3)=N2)=CC=C1)(F)F
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (11)

All In Vivo ImagingIn Vivo Efficacy StudyIFWBBio/Physico-chemical Assay

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Journal Impact Factor

Nat Commun

Astrocyte-derived clusterin disrupts glial physiology to obstruct remyelination in mouse models of demyelinating diseases. [Abstract]

2024 Sep 6;15(1):7791. PMID: 39242637
Adv Sci (Weinh)

Diabetes Mellitus Facilitates Gallstone Formation Through CXCR2-NETs-Mediated Liver-Bile Barrier Damage. [Abstract]

2026 Apr;13(24):e19500. PMID: 41698066

SEW2871 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2026 Apr;13(24):e19500. [Abstract]

Representative images of gallbladders from mice fed a lithogenic diet (LD), lithogenic diet with SEW2871 (0.5 mg/kg, daily, 4 weeks) intraperitoneal injection (LD+SEW2871), diabetic lithogenic diet (DMLD) and diabetic lithogenic diet with SEW2871 intraperitoneal injection.
J Renin Angiotensin Aldosterone Syst

2025 Oct 16.
Neurosci Bull

Alpha-synuclein Fibrils Inhibit Activation of the BDNF/ERK Signaling Loop in the mPFC to Induce Parkinson's Disease-like Alterations with Depression. [Abstract]

2025 Jun;41(6):951-969. PMID: 39609371

SEW2871 purchased from MedChemExpress. Usage Cited in: Neurosci Bull. 2025 Jun;41(6):951-969. [Abstract]

In the forced swimming test, the rats in the SEW2871 group swam for a longer duration and spent less time immobilized. Rats were given SEW2871 (0.5 mg/kg) or the isovolumetric solvent daily by intraperitoneal injection for 14 days after mode completion. Meanwhile, SEW2871 (1 μmol/L) or isovolumetric solvent was added to the primary PFC neurons and incubated for 48 h, after which tests were carried out.

SEW2871 purchased from MedChemExpress. Usage Cited in: Neurosci Bull. 2025 Jun;41(6):951-969. [Abstract]

In the new object recognition test, the rats in the SEW2871 group spent more time exploring the new object and had a higher recognition index than those in the PFF group.

SEW2871 purchased from MedChemExpress. Usage Cited in: Neurosci Bull. 2025 Jun;41(6):951-969. [Abstract]

We applied immunofluorescence staining to the mPFC region and found that treatment with SEW2871 partially restored the expression levels of BDNF.

SEW2871 purchased from MedChemExpress. Usage Cited in: Neurosci Bull. 2025 Jun;41(6):951-969. [Abstract]

SEW2871 upregulates the levels of phosphorylation of ERK and its downstream proteins. The Lewy body-like inclusions showed the opposite alterations.

SEW2871 purchased from MedChemExpress. Usage Cited in: Neurosci Bull. 2025 Jun;41(6):951-969. [Abstract]

SEW2871 somewhat mitigates the decrease in DA content.
Am J Physiol Cell Physiol

Glycolytic enzyme PFKFB3 regulates sphingosinae 1-phosphate receptor 1 in proangiogenic glomerular endothelial cells under diabetic condition. [Abstract]

2023 Nov 1;325(5):C1354-C1368. PMID: 37781737
iScience

Broad and diverse roles of sphingosine-1-phosphate/sphingosine-1-phosphate receptors in the prostate. [Abstract]

2024 Oct 30;27(12):111290. PMID: 39618500
Naunyn Schmiedebergs Arch Pharmacol

Label-free cell phenotypic profiling of sphingosine-1-phosphate receptor 1 and discovery of its agonist from natural products. [Abstract]

2026 May 29. PMID: 42209736
Reprod Biomed Online

Blocking sphingosine 1-phosphate receptor 1 with modulators reduces immune cells infiltration and alleviates endometriosis in mice. [Abstract]

2023 Nov;47(5):103304. PMID: 37757611
bioRxiv

2026 Jun 29.
bioRxiv

Gz Enhanced Signal Transduction assaY (GZESTY) for GPCR deorphanization. [Abstract]

2024 Jul 26:2024.07.26.605282. PMID: 39091869
Research Square Preprint

2023 Dec 1.

Solvent & Solubility

In Vitro:

DMF : 100 mg/mL (227.09 mM; Need ultrasonic)

DMSO : 25 mg/mL (56.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 20 mg/mL (45.42 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.2709 mL

11.3543 mL

22.7087 mL

5 mM

0.4542 mL

2.2709 mL

4.5417 mL

10 mM

0.2271 mL

1.1354 mL

2.2709 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (4.72 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (4.72 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

Protocol 3

Add each solvent one by one: 10% EtOH 90% Corn Oil

Solubility: ≥ 2 mg/mL (4.54 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (20.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocols

All Histomorphology Signal Pathway Related

Fibrosis/Collagen Morphometry
Fibrosis and collagen morphometry is based on the quantitative visualization of fibrillar collagen deposition in tissue sections using histochemical stains such as Sirius Red (Picrosirius Red) or Masson's trichrome, followed by image-based or polarization-enhanced analysis to estimate collagen proportional area as a surrogate of extracellular matrix accumulation during fibrotic remodeling. Sirius Red combined with polarized light microscopy enhances detection of collagen fibers due to birefringence properties, enabling more specific visualization of collagen type I and III fibrils compared to conventional bright-field histology, while whole-section or region-restricted digital morphometry reduces field-selection bias in fibrosis assessment. Alternative quantitative approaches include second harmonic generation (SHG) and two-photon excited fluorescence microscopy, which enable label-free detection of fibrillar collagen and have been validated against histological staining and biochemica
Collagen: Sirius Red Staining
Sirius Red or picrosirius red staining is a histochemical method for visualizing collagen-rich extracellular matrix in tissue sections, and collagen fibers are detected as red-stained structures under bright-field microscopy with enhanced birefringence under polarized light. Picrosirius red is useful for assessing total collagen organization, distribution, and fibrosis burden, but polarized color should not be interpreted as a definitive collagen type I versus type III readout because color is affected by fiber orientation, thickness, and packing.
Connective Tissue: Masson's Trichrome/Collagen Trichrome Staining
Masson’s Trichrome (collagen/trichrome staining) is a histological technique that differentially stains tissue compartments using sequential acidic dyes to distinguish collagen from muscle and cytoplasmic components based on dye affinity and tissue permeability differences, enabling visualization of fibrosis and connective tissue architecture in histological sections. The classical formulation typically uses Weigert's iron hematoxylin for nuclear staining, Biebrich scarlet-acid fuchsin for cytoplasm and muscle, and aniline blue (or light green variants) for collagen, producing a characteristic blue/green collagen signal contrasted against red cytoplasm and dark nuclei. The staining principle relies on selective displacement of smaller dye molecules by larger anionic dyes in collagen-rich regions under controlled acidified conditions, which enhances collagen-specific dye retention. This property makes the method widely used for fibrosis assessment in organs such as heart, liver, lung, a
Research Protocol for Metabolic Diseases
AMP-activated protein kinase, AMPK, is a conserved cellular energy sensor that responds to reduced cellular energy status and coordinates metabolism by increasing ATP-generating catabolic pathways while suppressing ATP-consuming anabolic processes. In metabolic disease research, the AMPK pathway is experimentally relevant because it regulates hepatic lipid synthesis, fatty acid oxidation, glucose production, skeletal-muscle glucose disposal, mTORC1-linked biosynthesis, autophagy, mitochondrial homeostasis, and whole-body energy balance. The central pathway logic is that energy stress, metformin, exercise-like stimulation, or direct AMPK activators increase AMPKα Thr172 phosphorylation and downstream substrate phosphorylation, including ACC and RAPTOR. Phosphorylation of ACC suppresses lipogenesis and supports fatty acid oxidation, whereas phosphorylation of RAPTOR suppresses mTORC1 signaling and links cellular energy status to growth and protein synthesis control. The pathway is linked
Liver Histomorphometry
Liver histomorphometry is a quantitative histological approach used to measure structural alterations in hepatic tissue, including parenchymal loss, steatosis, fibrosis, and vascular remodeling, by combining stained tissue section analysis with stereological or computerized image-based measurements. Classical morphometric frameworks quantify volume fractions of liver compartments and fibrotic regions using systematic sampling and image analysis, enabling objective comparison of pathological changes across experimental groups. These approaches are widely applied in liver cirrhosis and fibrosis studies to reduce subjectivity in histological scoring and improve reproducibility of tissue evaluation. Recent methodological advances integrate automated image analysis and radiomics-based extraction of histological features from standard liver stains (e. g. , H&E and fibrotic stains), enabling quantitative correlation between morphometric features and fibrosis stages in non-alcoholic fatty live

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (479 KB)
- English - EN (479 KB)
- Français - FR (479 KB)
- Deutsch - DE (479 KB)
- Norwegian - NO (479 KB)
- Español - ES (479 KB)
- Swedish - SV (479 KB)
- Italian - IT (479 KB)
- Korean - KR (479 KB)
- Portuguese - PT (479 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Park SJ, et al. Sphingosine 1-Phosphate Receptor Modulators and Drug Discovery. Biomol Ther (Seoul). 2017 Jan 1;25(1):80-90. [Content Brief]

[2]. Jo E, et al. S1P1-selective in vivo-active agonists from high-throughput screening: off-the-shelf chemical probes of receptor interactions, signaling, and fate. Chem Biol. 2005 Jun;12(6):703-15. [Content Brief]

[3]. Dong J, et al. Oral treatment with SEW2871, a sphingosine-1-phosphate type 1 receptor agonist, ameliorates experimental colitis in interleukin-10 gene deficient mice. Clin Exp Immunol. 2014 Jul;177(1):94-101. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
Ethanol / DMSO / DMF	1 mM	2.2709 mL	11.3543 mL	22.7087 mL	56.7717 mL
	5 mM	0.4542 mL	2.2709 mL	4.5417 mL	11.3543 mL
	10 mM	0.2271 mL	1.1354 mL	2.2709 mL	5.6772 mL
	15 mM	0.1514 mL	0.7570 mL	1.5139 mL	3.7848 mL
	20 mM	0.1135 mL	0.5677 mL	1.1354 mL	2.8386 mL
	25 mM	0.0908 mL	0.4542 mL	0.9083 mL	2.2709 mL
	30 mM	0.0757 mL	0.3785 mL	0.7570 mL	1.8924 mL
	40 mM	0.0568 mL	0.2839 mL	0.5677 mL	1.4193 mL
DMSO / DMF	50 mM	0.0454 mL	0.2271 mL	0.4542 mL	1.1354 mL
DMF	60 mM	0.0378 mL	0.1892 mL	0.3785 mL	0.9462 mL
	80 mM	0.0284 mL	0.1419 mL	0.2839 mL	0.7096 mL
	100 mM	0.0227 mL	0.1135 mL	0.2271 mL	0.5677 mL

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SEW2871

Publications Citing Use of MedChemExpress (MCE) SEW2871

All LPL Receptor Isoforms

All ERK Isoforms

All Akt Isoforms