Cat. No.: HY-135885

VBIT-12

8 Cited Publications

Customer Review

Based on 8 publication(s) in Google Scholar

VBIT-12 is a potent VDAC1 inhibitor. VBIT-12 directly interacts with VDAC1 and prevents VDAC1 oligomerization, and thus inhibits apoptotic action of VDAC1.

For research use only. We do not sell to patients.

Purity: 98.87%
CAS No.: 2089227-65-4
Formula: C₂₅H₂₇N₃O₃
Molecular Weight:417.50

Storage:

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

VBIT-12 Chemical Structure

CAS No.: 2089227-65-4

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (8)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

VBIT-12 is a potent VDAC1 inhibitor. VBIT-12 directly interacts with VDAC1 and prevents VDAC1 oligomerization, and thus inhibits apoptotic action of VDAC1^[1].

IC₅₀ & Target

VDAC1

All VDAC Isoforms :

In Vitro

VBIT-12 inhibits VDAC1 oligomerization, hexokinase (HK) detachment and apoptotic cell death^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 2089227-65-4
Appearance Solid
Molecular Weight 417.50
Formula C₂₅H₂₇N₃O₃
Color White to off-white
SMILES

O=C(O)CNC(C1(NC2=CC=CC=C2)CCN(CC3=C4C=CC=CC4=CC=C3)CC1)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (8)

All OthersWBIFCell Proliferation/Viability AssayIP

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Journal Impact Factor

Sci Adv

Retromer promotes the lysosomal turnover of mtDNA. [Abstract]

2025 Apr 4;11(14):eadr6415. PMID: 40184468

VBIT-12 purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Apr 4;11(14):eadr6415. [Abstract]

The role of mitochondrial membrane pores in mtDNA release was analyzed. Under specified conditions, 24 hours after transfection, cells were treated for 4 hours with 10 μM chloroquine, 25 μM VBIT-12 (to inhibit VDAC1), or 100 μM V5 peptide (to inhibit BAX oligomerization).
Phytomedicine

Delphinidin targets voltage-dependent anion channel 1 to inhibit ferroptosis and protect against retinal photochemical damage. [Abstract]

2026 Jun 17:159:158455. PMID: 42335641
Free Radic Biol Med

PGAM1-dependent VDAC1 oligomerization disrupts mitochondrial quality control to drive doxorubicin cardiotoxicity via the cGAS-STING-ferroptosis axis. [Abstract]

2026 Apr:247:71-94. PMID: 41651300
Free Radic Biol Med

2025 Oct 25.

VBIT-12 purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2025 Oct 25.

Inhibition of VDAC1 oligomerization maintained RPE function in vitro. ARPE-19 cells were pretreated with 10 μM VBIT-12 for 2 h, followed by exposure to 10 mM NaIO3 for 24 h. Western blots of EGS-treated VDAC1 monomers and oligomers were obtained from ARPE-19 cells in the control group, NaIO3-induced group, and NaIO3 and VBIT-12 pretreated groups.

VBIT-12 purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2025 Oct 25.

Inhibition of VDAC1 oligomerization maintained RPE function in vitro. ARPE-19 cells were pretreated with 10 μM VBIT-12 for 2 h, followed by exposure to 10 mM NaIO3 for 24 h. Representative immunofluorescence images of ZO-1 from each group are shown. Cell nuclei were counterstained with DAPI. The figure shows the quantitative analysis results of ZO-1 fluorescence intensity.

VBIT-12 purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2025 Oct 25.

Inhibition of VDAC1 oligomerization maintained RPE function in vitro. ARPE-19 cells were pretreated with 10 μM VBIT-12 for 2 hours, followed by exposure to 10 mM NaIO3 for 24 hours. Cell viability was assessed using the CCK-8 assay in each group.

VBIT-12 purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2025 Oct 25.

Co-IP assay demonstrating the physical association of STING with major PANoptosome components (NLRP3, Caspase-8, and RIPK3) in NaIO3-induced ARPE-19 cells with or without VBIT-12 intervention.
Environ Pollut

Targeting mitochondrial permeability transition pore ameliorates PM2.5-induced mitochondrial dysfunction in airway epithelial cells. [Abstract]

2022 Feb 15;295:118720. PMID: 34953947
J Ethnopharmacol

Liquiritigenin alleviates inflammation and mitochondrial dysfunction in acute pancreatitis via ERβ-VDAC1 signaling. [Abstract]

2025 Sep 3:120526. PMID: 40912483
Cell Biol Toxicol

LvHemB1, a novel cationic antimicrobial peptide derived from the hemocyanin of Litopenaeus vannamei, induces cancer cell death by targeting mitochondrial voltage-dependent anion channel 1. [Abstract]

2022 Feb;38(1):87-110. PMID: 33630204
Discov Oncol

VDAC1 as prognostic marker and therapeutic target in lung adenocarcinoma: a study integrating bioinformatics and experimental validation. [Abstract]

2025 Nov 19;16(1):2129. PMID: 41258557

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (598.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3952 mL

11.9760 mL

23.9521 mL

5 mM

0.4790 mL

2.3952 mL

4.7904 mL

10 mM

0.2395 mL

1.1976 mL

2.3952 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (4.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (4.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (4.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (537 KB)
- English - EN (537 KB)
- Français - FR (537 KB)
- Deutsch - DE (537 KB)
- Norwegian - NO (537 KB)
- Español - ES (537 KB)
- Swedish - SV (537 KB)
- Italian - IT (537 KB)
- Korean - KR (537 KB)
- Portuguese - PT (537 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Shoshan-Barmatz V, et al. VDAC1, mitochondrial dysfunction, and Alzheimer's disease. Pharmacol Res. 2018 May;131:87-101. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.3952 mL	11.9760 mL	23.9521 mL	59.8802 mL
	5 mM	0.4790 mL	2.3952 mL	4.7904 mL	11.9760 mL
	10 mM	0.2395 mL	1.1976 mL	2.3952 mL	5.9880 mL
	15 mM	0.1597 mL	0.7984 mL	1.5968 mL	3.9920 mL
	20 mM	0.1198 mL	0.5988 mL	1.1976 mL	2.9940 mL
	25 mM	0.0958 mL	0.4790 mL	0.9581 mL	2.3952 mL
	30 mM	0.0798 mL	0.3992 mL	0.7984 mL	1.9960 mL
	40 mM	0.0599 mL	0.2994 mL	0.5988 mL	1.4970 mL
	50 mM	0.0479 mL	0.2395 mL	0.4790 mL	1.1976 mL
	60 mM	0.0399 mL	0.1996 mL	0.3992 mL	0.9980 mL
	80 mM	0.0299 mL	0.1497 mL	0.2994 mL	0.7485 mL
	100 mM	0.0240 mL	0.1198 mL	0.2395 mL	0.5988 mL

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VBIT-12

Membrane Transporter/Ion Channel Compound Library

Highly Selective Inhibitors Library

Ion Channel Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) VBIT-12

All VDAC Isoforms

Biological Activity

Chemical Information

Publications (8)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

VBIT-12

Membrane Transporter/Ion Channel Compound Library

Highly Selective Inhibitors Library

Ion Channel Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) VBIT-12

All VDAC Isoforms

Biological Activity

Chemical Information

Publications (8)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

VBIT-12 Related Classifications

HY-135885 Related Classifications