W-5 hydrochloride
Based on 1 Customer Validation
W-5 hydrochloride is a calmodulin antagonist. W-5 hydrochloride inhibits phosphodiesterase activation with an IC50 value of 240 µM. W-5 hydrochloride can be used as a negative control for W-7 (HY-100912).
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- Pureté : 99.93%
- CAS No.: 61714-25-8
- Formule: C16H23ClN2O2S
- Masse moléculaire:342.88
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Stockage:
-20°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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HY-100912
W-7 hydrochlorideCalmodulin Antagonist -
HY-W009724
2-Aminoethyl diphenylborinateIP3R Inhibitor -
HY-100910
W-13 hydrochlorideCalmodulin Antagonist -
HY-B0124
ZonisamideCarbonic Anhydrase Inhibitor -
HY-14657
Dantrolene sodiumCalcium Channel Inhibitor -
HY-14650
DHEASteroid Hormone -
HY-13434
IonomycinCalcium Ionophore -
HY-N6687
CalcimycinCation Ionophore -
HY-13434A
Ionomycin calciumCalcium Ionophore -
HY-100912R
W-7 hydrochloride (Standard)Calmodulin Antagonist -
HY-B0317
AmlodipineCalcium Channel Blocker -
HY-A0064
Verapamil hydrochlorideCalcium Channel Blocker
Activité biologique
Description
IC50 & Target
IC50: 240 µM (phosphodiesterase)[1]
In Vitro
W-5 hydrochloride (40 µM; 24 h; incubation with 10 μg/mL of apolipoprotein A-I (apoA-I)) increases the cholesterol and choline-phospholipid efflux[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 61714-25-8
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Appearance Solid
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Masse moléculaire 342.88
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Formule C16H23ClN2O2S
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Color White to off-white
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SMILES
O=S(C1=C2C=CC=CC2=CC=C1)(NCCCCCCN)=O.[H]Cl
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
-20°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
Solvant et solubilité
In Vitro:
DMSO : 50 mg/mL (145.82 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (3.65 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (3.65 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (273 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Suzuki S, et al. Enhancement of the cAMP-induced apolipoprotein-mediated cellular lipid release by calmodulin inhibitors W7 and W5 from RAW 264 mouse macrophage cell line cells. J Cardiovasc Pharmacol. 2000 Nov;36(5):609-16. [Content Brief]
[2]. Yokokura S, et al. Calmodulin antagonists induce cell cycle arrest and apoptosis in vitro and inhibit tumor growth in vivo in human multiple myeloma. BMC Cancer. 2014 Nov 26;14:882. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9165 mL | 14.5824 mL | 29.1647 mL | 72.9118 mL |
| 5 mM | 0.5833 mL | 2.9165 mL | 5.8329 mL | 14.5824 mL | |
| 10 mM | 0.2916 mL | 1.4582 mL | 2.9165 mL | 7.2912 mL | |
| 15 mM | 0.1944 mL | 0.9722 mL | 1.9443 mL | 4.8608 mL | |
| 20 mM | 0.1458 mL | 0.7291 mL | 1.4582 mL | 3.6456 mL | |
| 25 mM | 0.1167 mL | 0.5833 mL | 1.1666 mL | 2.9165 mL | |
| 30 mM | 0.0972 mL | 0.4861 mL | 0.9722 mL | 2.4304 mL | |
| 40 mM | 0.0729 mL | 0.3646 mL | 0.7291 mL | 1.8228 mL | |
| 50 mM | 0.0583 mL | 0.2916 mL | 0.5833 mL | 1.4582 mL | |
| 60 mM | 0.0486 mL | 0.2430 mL | 0.4861 mL | 1.2152 mL | |
| 80 mM | 0.0365 mL | 0.1823 mL | 0.3646 mL | 0.9114 mL | |
| 100 mM | 0.0292 mL | 0.1458 mL | 0.2916 mL | 0.7291 mL |
W-5 hydrochloride Related Classifications
HY-100911 Related Classifications
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