2. PROTAC Apoptosis Epigenetics
  3. PROTACs Apoptosis METTL3
  4. WD6305

WD6305 is a potent and selective METTL3-METTL14 PROTAC degrader. WD6305 has DC50 values of 140 nM and 194 nM for METTL3 and METTL14, respectively. WD6305 inhibits m6A modification and proliferation of AML cells, and induces apoptosis. WD6305 has antitumor activity.(Pink: UZH2 (HY-115717); Black: Linker; Blue: VHL ligand (HY-150803))

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WD6305

WD6305 Chemical Structure

CAS No. : 3066823-45-5

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Based on 2 publication(s) in Google Scholar

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WD6305 Related Antibodies

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von Hippel-Lindau (VHL) Cereblon MDM2 cIAP1

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Description

WD6305 is a potent and selective METTL3-METTL14 PROTAC degrader. WD6305 has DC50 values of 140 nM and 194 nM for METTL3 and METTL14, respectively. WD6305 inhibits m6A modification and proliferation of AML cells, and induces apoptosis. WD6305 has antitumor activity[1].(Pink: UZH2 (HY-115717); Black: Linker; Blue: VHL ligand (HY-150803))

IC50 & Target

DC50: 140 nM (METTL3); D max: 91.9% (METTL3) [1]
DC50: 194 nM (METTL14)[1]
In Vitro

WD6305 (20-5000 nM; 24 h) reduces METTL3 protein levels in Mono-Mac-6 cells with a DC50 of 140 nM and a D max of 91.9%[1].
WD6305 (20-5000 nM; 24 h) reduces METTL14 protein levels in Mono-Mac-6 cells with a DC50 of 194 nM[1].
WD6305 (0.5-10 μM; 48 h) decreases the ratio of m6A to polyA mRNA, inhibits cell proliferation and induces cell apoptosis in Mono-Mac-6 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

WD6305 Related Antibodies

Western Blot Analysis[1]

Cell Line: Mono-Mac-6 cells
Concentration: 20, 50, 100, 250, 500, 1000, 2000 and 5000 nM
Incubation Time: 24 h
Result: Reduced METTL3 and METTL14 protein levels in a dose-dependent manner.

Western Blot Analysis[1]

Cell Line: Mono-Mac-6 cells
Concentration: 1 and 2.5 μM
Incubation Time: 48 h
Result: Increased the levels of cleaved caspase-3 and cleaved PARP.
Molecular Weight

1112.38

Formula

C61H75F2N11O5S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(N[C@@H](C(C)(C)C)C(N1[C@H](C(N[C@@H](C)C2=CC=C(C3=C(C)N=CS3)C=C2)=O)C[C@@H](O)C1)=O)CCC#CC(C=C4)=CC=C4CNC5=CC(N(CC6)CCC76NC(CN(C8=CC(F)=C(CN9CCC(C)(C)CC9)C=C8F)C7)=O)=NC=N5
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (89.90 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.8990 mL 4.4949 mL 8.9897 mL
5 mM 0.1798 mL 0.8990 mL 1.7979 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation

Purity: 98.98%

SDS (251 KB)

COA (249 KB) HNMR (241 KB) LCMS (95 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.8990 mL 4.4949 mL 8.9897 mL 22.4743 mL
5 mM 0.1798 mL 0.8990 mL 1.7979 mL 4.4949 mL
10 mM 0.0899 mL 0.4495 mL 0.8990 mL 2.2474 mL
15 mM 0.0599 mL 0.2997 mL 0.5993 mL 1.4983 mL
20 mM 0.0449 mL 0.2247 mL 0.4495 mL 1.1237 mL
25 mM 0.0360 mL 0.1798 mL 0.3596 mL 0.8990 mL
30 mM 0.0300 mL 0.1498 mL 0.2997 mL 0.7491 mL
40 mM 0.0225 mL 0.1124 mL 0.2247 mL 0.5619 mL
50 mM 0.0180 mL 0.0899 mL 0.1798 mL 0.4495 mL
60 mM 0.0150 mL 0.0749 mL 0.1498 mL 0.3746 mL
80 mM 0.0112 mL 0.0562 mL 0.1124 mL 0.2809 mL
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WD6305 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

WD63053066823-45-5WD 6305WD-6305PROTACsApoptosisMETTL3Methyltransferase-like 3AMLMono-Mac-6 cellsInhibitorinhibitorinhibit

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