β-Elemene (Synonyms: (-)-β-Elemene; Levo-β-elemene)

Name: β-Elemene
Brand: MedChemExpress
SKU: HY-107324
Rating: 5.0 (6 reviews)

Cat. No.: HY-107324 Purity: 99.62%: Data Sheet
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β-Elemene ((-)-β-Elemene; Levo-β-elemene) is isolated from natural plant Curcuma aromatica with an antitumor activity. β-Elemene can induce cell apoptosis.

For research use only. We do not sell to patients.

CAS No. : 515-13-9

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Based on 6 publication(s) in Google Scholar

Other Forms of β-Elemene:

β-Elemene (Standard) In-stock

6 Publications Citing Use of MCE β-Elemene

Cell Proliferation/Viability Assay

RT-PCR

: β-Elemene purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 May 18;10(10):e31537.; CCK-8 to assess the proliferation of PHEGFs after β-elemene (0-640=-640Μm, 0-72 h) intervention.

: β-Elemene purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 May 18;10(10):e31537.; β-elemene (480 μM) suppressed the proliferation and promoted the apoptosis of PHEGFs. Flod change of FAP, p-PTEN, p-PI3K, and p-AKT.

: β-Elemene purchased from MedChemExpress. Usage Cited in: Sci Rep. 2023 Jul 27;13(1):12160. [Abstract]; Effects of β-elemene (0-400 μM, 0-72 h) on cell viability and mRNA expression of key targets in ECA-109 cells. Here showed the results of cell viability experiments.

: β-Elemene purchased from MedChemExpress. Usage Cited in: Sci Rep. 2023 Jul 27;13(1):12160. [Abstract]; Effects of β-elemene (0-400 μM) on cell viability and mRNA expression of key targets in ECA-109 cells. Here showed the results of the effects of β-elemene on STAT3 mRNA expressions in ECA-109 cells.

: β-Elemene purchased from MedChemExpress. Usage Cited in: Sci Rep. 2023 Jul 27;13(1):12160. [Abstract]; Effects of β-elemene (0-200 μM) on protein expression levels of key targets in ECA-109 cells. Here showed the results of the protein expression levels of STAT3, EGFR, BCL2L1, and CASP9 analyzed by western blot.

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Biological Activity
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Description

β-Elemene ((-)-β-Elemene; Levo-β-elemene) is isolated from natural plant Curcuma aromatica with an antitumor activity. β-Elemene can induce cell apoptosis.

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	227 μM Compound: 1	Antiproliferative activity against human A549 cells assessed as inhibition of cell growth measured after 48 hrs by CCK8 assay relative to control Antiproliferative activity against human A549 cells assessed as inhibition of cell growth measured after 48 hrs by CCK8 assay relative to control	[PMID: 36240626]
HCT-116	IC₅₀	781.9 μM Compound: 1	Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell growth measured after 48 hrs by CCK8 assay relative to control Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell growth measured after 48 hrs by CCK8 assay relative to control	[PMID: 36240626]
HeLa	IC₅₀	213.51 μM Compound: beta-Elemene	Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay	[PMID: 28463784]
HeLa	IC₅₀	236.2 μM Compound: beta-elemene	Antiproliferative activity against human HeLa cells after 24 hrs by WST-1 assay Antiproliferative activity against human HeLa cells after 24 hrs by WST-1 assay	[PMID: 19128976]
PC-3	IC₅₀	276.2 μM Compound: 1	Antiproliferative activity against human PC-3 cells assessed as inhibition of cell growth measured after 48 hrs by CCK8 assay relative to control Antiproliferative activity against human PC-3 cells assessed as inhibition of cell growth measured after 48 hrs by CCK8 assay relative to control	[PMID: 36240626]
SGC-7901	IC₅₀	236.27 μM Compound: beta-Elemene	Antiproliferative activity against human SGC7901 cells after 72 hrs by MTT assay Antiproliferative activity against human SGC7901 cells after 72 hrs by MTT assay	[PMID: 28463784]
U-251	IC₅₀	180.8 μM Compound: 1	Antiproliferative activity against human U-251 cells assessed as inhibition of cell growth measured after 48 hrs by CCK8 assay relative to control Antiproliferative activity against human U-251 cells assessed as inhibition of cell growth measured after 48 hrs by CCK8 assay relative to control	[PMID: 36240626]
U-87MG ATCC	IC₅₀	179.72 μM Compound: beta-Elemene	Antiproliferative activity against human U87 cells after 72 hrs by MTT assay Antiproliferative activity against human U87 cells after 72 hrs by MTT assay	[PMID: 28463784]
U-87MG ATCC	IC₅₀	266.3 μM Compound: 1	Antiproliferative activity against human U-87 MG cells assessed as inhibition of cell growth measured after 48 hrs by CCK8 assay relative to control Antiproliferative activity against human U-87 MG cells assessed as inhibition of cell growth measured after 48 hrs by CCK8 assay relative to control	[PMID: 36240626]

In Vitro

β-Elemene (0-200 μg/ml; 24 hours) shows IC₅₀ values of 72.8 μg/ml; 47.4 μg/ml; 61.5 μg/ml; 3.661 μg/ml; 68 μg/ml; 72.12 μg/ml; 37.894 μg/ml and 37.703 μg/ml for SV-HUC-1, T24, 5637, TCCSUP, J82,UMUC-3,RT4, and SW780 cells, respectively^[1].
β-Elemene (0-75 μg/ml; 24 hours) decreases cell number from 50 μg/ml and is notably decreased at 75 μg/ml, induces dose-dependent G1-phase arrest in T24cells and significantly reduces the percentage of cells in the S-phase^[1].
β-Elemene (50 μg/ml; 12 hours) downregulates the expression levels of p-STAT3 and the cell cycle-related proteins cyclin D1, CDK4 and CDK6, and upregulates p21 and p27expression in T24 cells ^[1].
β-Elemene (50 μg/ml; 24 hours) enhances cisplatin-induced apoptosis by activating the ROS-AMPK signaling pathway^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	T24, 5637, TCCSUP, J82, UM-UC-3, RT4 and SW780 cells
Concentration:	0, 6.25, 12.5, 25, 50, 100, 150 and 200 µg/ml
Incubation Time:	24 hours
Result:	Inhibited the proliferation of RT4, SW780, J82, UMUC-3, TCCSUP, 5637 and T24 human bladder cancer cells and SV-HUC-1 human urothelial cells.

Cell Cycle Analysis^[2]

Cell Line:	T24 and 5637 cells
Concentration:	0, 25, 50 and 75 µg/ml
Incubation Time:	24 hours
Result:	Induced the percentage of cells in the S-phase as a dose-dependent manner.

Western Blot Analysis^[2]

Cell Line:	T24 cells
Concentration:	50 µg/ml
Incubation Time:	24 hours
Result:	Downregulated p-STAT3 and cell cycle-related proteins.

Clinical Trial

Molecular Weight

204.35

Formula

C₁₅H₂₄

CAS No.

515-13-9

Appearance

Liquid (Density: 0.862 g/cm³)

Color

Colorless to light yellow

SMILES

CC([C@H]1[C@@](C)(CC[C@@H](C(C)=C)C1)C=C)=C

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

Ethanol : 50 mg/mL (244.68 mM; Need ultrasonic)

DMSO : 50 mg/mL (244.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.8936 mL	24.4678 mL	48.9356 mL
5 mM	0.9787 mL	4.8936 mL	9.7871 mL
10 mM	0.4894 mL	2.4468 mL	4.8936 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (12.23 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (12.23 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (12.23 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.62%

Select Batch:

Data Sheet (279 KB) SDS (726 KB)

COA (250 KB) HNMR (248 KB) RP-HPLC (258 KB) MS (72 KB)

Handling Instructions (2659 KB)

References

[1]. Ross, S.A., and ElSohly, M.A. The volatile oil composition of fresh and air-dried buds of Cannabis sativa. J. Nat. Prod. 59(1), 49-51 (1996). [Content Brief]

[2]. Gan D, et al. β-elemene enhances cisplatin-induced apoptosis in bladder cancer cells through the ROS-AMPK signaling pathway.Oncol Lett. 2020 Jan;19(1):291-300. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO	1 mM	4.8936 mL	24.4678 mL	48.9356 mL	122.3391 mL
	5 mM	0.9787 mL	4.8936 mL	9.7871 mL	24.4678 mL
	10 mM	0.4894 mL	2.4468 mL	4.8936 mL	12.2339 mL
	15 mM	0.3262 mL	1.6312 mL	3.2624 mL	8.1559 mL
	20 mM	0.2447 mL	1.2234 mL	2.4468 mL	6.1170 mL
	25 mM	0.1957 mL	0.9787 mL	1.9574 mL	4.8936 mL
	30 mM	0.1631 mL	0.8156 mL	1.6312 mL	4.0780 mL
	40 mM	0.1223 mL	0.6117 mL	1.2234 mL	3.0585 mL
	50 mM	0.0979 mL	0.4894 mL	0.9787 mL	2.4468 mL
	60 mM	0.0816 mL	0.4078 mL	0.8156 mL	2.0390 mL
	80 mM	0.0612 mL	0.3058 mL	0.6117 mL	1.5292 mL
	100 mM	0.0489 mL	0.2447 mL	0.4894 mL	1.2234 mL

β-Elemene Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

β-Elemene515-13-9(-)-β-Elemene Levo-β-elemeneApoptosisInhibitorinhibitorinhibit

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β-Elemene (Synonyms: (-)-β-Elemene; Levo-β-elemene)

Customer Review

Other Forms of β-Elemene:

β-Elemene Related Antibodies

6 Publications Citing Use of MCE β-Elemene

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

β-Elemene Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

β-Elemene Related Classifications

Keywords:

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Customer Review

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