1. Metabolic Enzyme/Protease
  2. Endogenous Metabolite
  3. 5-Hydroxy-2'-deoxyuridine

5-Hydroxy-2'-deoxyuridine (Synonyms: 5-OHdU)

Cat. No.: HY-130801
Handling Instructions

5-Hydroxy-2'-deoxyuridine (5-OHdU) is a major stable oxidation product of 2'-Deoxycytidine. 5-Hydroxy-2'-deoxyuridine can be incorporated into DNA in vitro by DNA polymerase.

For research use only. We do not sell to patients.

5-Hydroxy-2'-deoxyuridine Chemical Structure

5-Hydroxy-2'-deoxyuridine Chemical Structure

CAS No. : 5168-36-5

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5-Hydroxy-2'-deoxyuridine (5-OHdU) is a major stable oxidation product of 2'-Deoxycytidine. 5-Hydroxy-2'-deoxyuridine can be incorporated into DNA in vitro by DNA polymerase[1].

IC50 & Target[1]

Human Endogenous Metabolite


In Vitro

To study the specificity of nucleotide incorporation opposite 5-hydroxypyrimidines in template DNA, 18- and 45-member oligodeoxyribonucleotides, containing an internal 2'-deoxy-5-hydroxyuridine (5-OHdU) in two different sequence contexts, were used. Translesion synthesis past 2'-deoxy-5-hydroxyuridine (5-OHdU) in both oligonucleotides occurred, but pauses both opposite, and one nucleotide prior to, the modified base in the template is observed. The specificity of nucleotide incorporation opposite 2'-deoxy-5-hydroxyuridine (5-OHdU) in the template is sequence context dependent. In one sequence context, dA is the principal nucleotide incorporated opposite 2'-deoxy-5-hydroxyuridine (5-OHdU). However in a second sequence context, dC is the predominant base incorporated opposite 2'-deoxy-5-hydroxyuridine (5-OHdU). In that same sequence context, dC is also the predominant nucleotide incorporated opposite 2'-deoxy-5-hydroxyuridine (5-OHdU)[1].

Molecular Weight







OC[[email protected]@H]1[[email protected]](C[[email protected]](N2C(NC(C(O)=C2)=O)=O)O1)O


Room temperature in continental US; may vary elsewhere.


Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

H2O : 10 mg/mL (40.95 mM; Need ultrasonic)

Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.0950 mL 20.4750 mL 40.9500 mL
5 mM 0.8190 mL 4.0950 mL 8.1900 mL
10 mM 0.4095 mL 2.0475 mL 4.0950 mL
*Please refer to the solubility information to select the appropriate solvent.
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5-Hydroxy-2'-deoxyuridine5-OHdUEndogenous Metabolite5-hydroxypyrimidinesG transversions5-OHdUTPpairingspecificityoxidationdAdCDNApolymeraseincorporatedInhibitorinhibitorinhibit

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