5-Iodo-cytidine
Based on 1 publication(s) in Google Scholar
5-Iodo-cytidine (5-Iodo-D-cytidine) is a purine nucleoside analog that acts as an intermediate in organic synthesis. 5-Iodo-cytidine serves as a chemically modified nucleotide to replace partial cytidine residues in in vitro transcription, enhancing the translation efficiency and stability of chemically modified mRNA. Purine nucleoside analogs exhibit broad anti-tumor activity and target indolent lymphoid malignancies. 5-Iodo-cytidine can be used in mRNA therapy and tumor-related research.
For research use only. We do not sell to patients.
- Purity: 99.89%
- CAS No.: 1147-23-5
- Formula: C9H12IN3O5
- Molecular Weight:369.11
-
Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 5-Iodo-cytidine
More
Biological Activity
The chemically modified BMP-2 mRNA, prepared by replacing cytidine with 5-Iodo-cytidine at a 7.5% ratio during in vitro transcription, enables efficient expression of BMP-2 protein in HEK293 cells, MC3T3 cells, and muscle-derived mesenchymal stem cells, and upregulates genes related to osteogenesis and angiogenesis[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 1147-23-5
-
Appearance Solid
-
Molecular Weight 369.11
-
Formula C9H12IN3O5
-
Color White to off-white
-
SMILES
O=C(N=C(N)C(I)=C1)N1[C@@H]2O[C@H](CO)[C@@H](O)[C@H]2O
-
Synonyms
5-Iodo-D-cytidine
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
-
Journal Impact Factor
-
Most Recent
-
Environ Sci Technol
IodoFinder: Machine Learning-Guided Recognition of Iodinated Chemicals in Nontargeted LC-MS/MS Analysis. [Abstract]2025 Mar 11;59(9):4530-4539. PMID: 40015982
Solvent & Solubility
DMSO : ≥ 100 mg/mL (270.92 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (13.55 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (13.55 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (290 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
References
[1]. Robak T, et al. Purine nucleoside analogs in the treatment of rarer chronic lymphoid leukemias. Current pharmaceutical design. 2012;18(23):3373-88. [Content Brief]
[2]. Rohloff JC, et al. Practical synthesis of cytidine-5-carboxamide-modified nucleotide reagents. Nucleosides, nucleotides & nucleic acids. 2015;34(3):180-98. [Content Brief]
[3]. Zhang W, et al. An Improved, Chemically Modified RNA Encoding BMP-2 Enhances Osteogenesis In Vitro and In Vivo. Tissue engineering. Part A. 2019 Jan;25(1-2):131-144. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7092 mL | 13.5461 mL | 27.0922 mL | 67.7305 mL |
| 5 mM | 0.5418 mL | 2.7092 mL | 5.4184 mL | 13.5461 mL | |
| 10 mM | 0.2709 mL | 1.3546 mL | 2.7092 mL | 6.7730 mL | |
| 15 mM | 0.1806 mL | 0.9031 mL | 1.8061 mL | 4.5154 mL | |
| 20 mM | 0.1355 mL | 0.6773 mL | 1.3546 mL | 3.3865 mL | |
| 25 mM | 0.1084 mL | 0.5418 mL | 1.0837 mL | 2.7092 mL | |
| 30 mM | 0.0903 mL | 0.4515 mL | 0.9031 mL | 2.2577 mL | |
| 40 mM | 0.0677 mL | 0.3387 mL | 0.6773 mL | 1.6933 mL | |
| 50 mM | 0.0542 mL | 0.2709 mL | 0.5418 mL | 1.3546 mL | |
| 60 mM | 0.0452 mL | 0.2258 mL | 0.4515 mL | 1.1288 mL | |
| 80 mM | 0.0339 mL | 0.1693 mL | 0.3387 mL | 0.8466 mL | |
| 100 mM | 0.0271 mL | 0.1355 mL | 0.2709 mL | 0.6773 mL |
5-Iodo-cytidine Related Classifications
HY-W008615 Related Classifications
Powered by Bioz