6PGD-IN-2
Based on 1 Customer Validation
6PGD-IN-2 is an uncompetitive 6-phosphogluconate dehydrogenase (6PGD) inhibitor with an IC50 of 5.1 μM. 6PGD-IN-2 disrupts 6PGD oligomerization in a substrate-dependent manner. 6PGD-IN-2 results in a decrease of NADPH and Ru-5-P production as well as DNA synthesis in A549 cells. 6PGD-IN-2 can used for the studies of non-small cell carcinoma and liver cancer.
For research use only. We do not sell to patients.
- Purity: 99.86%
- Formula: C16H9BrCl2FN3OS
- Molecular Weight:461.14
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Storage:
Store at room temperature, keep dry and cool.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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All DNA/RNA Synthesis Isoforms
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Biological Activity
Enpp-1-IN-27 (Compound 19n) (0-300 μM) exhibits non-competitive inhibition characteristics against both 6PG and NADP+, with inhibition constants (Ki) of 64 μM (6PG) and 9.5 μM (NADP+), respectively[1].
Enpp-1-IN-27 (0-10 μM, 72 h-14 d) inhibits A549 cells (antiviability potency with an EC50 of 9.0 μM) and Huh7 cells proliferation and colony formation[1].
6PGD-IN-2 (0-40 μM) reduces the NADPH/NADP+ ratio, Ru-5-P levels, and DNA synthesis in A549 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 461.14
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Formula C16H9BrCl2FN3OS
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Color White to off-white
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SMILES
O=C(NC1=NN=C(C2=CC=C(Br)C=C2F)S1)CC3=CC=C(Cl)C(Cl)=C3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Store at room temperature, keep dry and cool
In solvent -80°C 1 year -20°C 6 months
Solvent & Solubility
DMSO : 18 mg/mL (39.03 mM; Need warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.8 mg/mL (3.90 mM); Clear solution
This protocol yields a clear solution of ≥ 1.8 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (18.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1685 mL | 10.8427 mL | 21.6854 mL | 54.2135 mL |
| 5 mM | 0.4337 mL | 2.1685 mL | 4.3371 mL | 10.8427 mL | |
| 10 mM | 0.2169 mL | 1.0843 mL | 2.1685 mL | 5.4213 mL | |
| 15 mM | 0.1446 mL | 0.7228 mL | 1.4457 mL | 3.6142 mL | |
| 20 mM | 0.1084 mL | 0.5421 mL | 1.0843 mL | 2.7107 mL | |
| 25 mM | 0.0867 mL | 0.4337 mL | 0.8674 mL | 2.1685 mL | |
| 30 mM | 0.0723 mL | 0.3614 mL | 0.7228 mL | 1.8071 mL |
6PGD-IN-2 Related Classifications
HY-175492 Related Classifications
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