7-Epi 10-desacetyl paclitaxel
Based on 1 Customer Validation
7-Epi 10-desacetyl paclitaxel (7-epi-10-deacetyltaxol), a taxol derivative, exhibits cytotoxicity against HeLa cells with an IC50 of 85 μM.
For research use only. We do not sell to patients.
- Purity: 99.76%
- CAS No.: 78454-17-8
- Formula: C45H49NO13
- Molecular Weight:811.87
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A2780 | IC50 |
0.03 μM
Compound: 5
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Antiproliferative activity against human A2780 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
Antiproliferative activity against human A2780 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
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[PMID: 25682561] |
| A549 | IC50 |
0.011 μM
Compound: 5
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Antiproliferative activity against human A549 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
Antiproliferative activity against human A549 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
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[PMID: 25682561] |
| A549 | IC50 |
0.024 μM
Compound: 5
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Antiproliferative activity against human A549/CDDP cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
Antiproliferative activity against human A549/CDDP cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
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[PMID: 25682561] |
| HCT-8 | IC50 |
13 μM
Compound: 5
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Antiproliferative activity against human HCT8 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
Antiproliferative activity against human HCT8 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
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[PMID: 25682561] |
| HeLa | IC50 |
0.085 nM
Compound: 17
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Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 72 hrs by WST-8 dye based assay
Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 72 hrs by WST-8 dye based assay
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[PMID: 28240909] |
| HeLa | IC50 |
uM μM
Compound: 17
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Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 72 hrs by WST-8 dye based assay
Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 72 hrs by WST-8 dye based assay
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[PMID: 28240909] |
| HepG2 | IC50 |
0.095 μM
Compound: 5
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Antiproliferative activity against human HepG2 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
Antiproliferative activity against human HepG2 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
|
[PMID: 25682561] |
| KB | IC50 |
0.0015 μg/mL
Compound: 60
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Cytotoxicity against human KB cells
Cytotoxicity against human KB cells
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[PMID: 14987066] |
| KB | ED50 |
0.34 ng/mL
Compound: 10
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Cytotoxicity against human KB cells
Cytotoxicity against human KB cells
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[PMID: 7264680] |
| KB | ED50 |
3.4 x 10-2 μg/mL
Compound: 10
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Cytotoxicity against human KB cells
Cytotoxicity against human KB cells
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[PMID: 7264680] |
| MCF7 | IC50 |
0.012 μM
Compound: 5
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Antiproliferative activity against human MCF7 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
Antiproliferative activity against human MCF7 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
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[PMID: 25682561] |
| NIH3T3 | IC50 |
2.2 μM
Compound: 5
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Antiproliferative activity against mouse NIH/3T3 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
Antiproliferative activity against mouse NIH/3T3 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
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[PMID: 25682561] |
| SW480 | IC50 |
0.059 μM
Compound: 5
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Antiproliferative activity against human SW480 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
Antiproliferative activity against human SW480 cells assessed as growth inhibition after 48 hrs by sulforhodamine B assay
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[PMID: 25682561] |
Chemical Information
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CAS No. 78454-17-8
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Appearance Solid
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Molecular Weight 811.87
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Formula C45H49NO13
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Color White to yellow
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SMILES
O=C(N[C@@H](C1=CC=CC=C1)[C@@H](O)C(O[C@H]2C[C@@](O)([C@@H](OC(C3=CC=CC=C3)=O)[C@@]4([H])[C@@]5(C)[C@H](O)C[C@]6([H])[C@@]4(OC(C)=O)CO6)C(C)(C)C([C@@H](O)C5=O)=C2C)=O)C7=CC=CC=C7
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Synonyms
7-epi-10-deacetyltaxol; 10-Deacetyl-7-epipaclitaxel
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 50 mg/mL (61.59 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.08 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (418 KB)
- English - EN (418 KB)
- Français - FR (418 KB)
- Deutsch - DE (418 KB)
- Norwegian - NO (418 KB)
- Español - ES (418 KB)
- Swedish - SV (418 KB)
- Italian - IT (418 KB)
- Korean - KR (418 KB)
- Portuguese - PT (418 KB)
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Handling Instructions (2659 KB)
References
[1]. Fungal 7-epi-10-deacetyltaxol produced by an endophytic Pestalotiopsis microspora induces apoptosis in human hepatocellular carcinoma cell line (HepG2). [Content Brief]
[2]. Dang PH, et al.α-Glucosidase Inhibitory and Cytotoxic Taxane Diterpenoids from the Stem Bark of Taxus wallichiana. J Nat Prod. 2017 Apr 28;80(4):1087-1095. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2317 mL | 6.1586 mL | 12.3172 mL | 30.7931 mL |
| 5 mM | 0.2463 mL | 1.2317 mL | 2.4634 mL | 6.1586 mL | |
| 10 mM | 0.1232 mL | 0.6159 mL | 1.2317 mL | 3.0793 mL | |
| 15 mM | 0.0821 mL | 0.4106 mL | 0.8211 mL | 2.0529 mL | |
| 20 mM | 0.0616 mL | 0.3079 mL | 0.6159 mL | 1.5397 mL | |
| 25 mM | 0.0493 mL | 0.2463 mL | 0.4927 mL | 1.2317 mL | |
| 30 mM | 0.0411 mL | 0.2053 mL | 0.4106 mL | 1.0264 mL | |
| 40 mM | 0.0308 mL | 0.1540 mL | 0.3079 mL | 0.7698 mL | |
| 50 mM | 0.0246 mL | 0.1232 mL | 0.2463 mL | 0.6159 mL | |
| 60 mM | 0.0205 mL | 0.1026 mL | 0.2053 mL | 0.5132 mL |
7-Epi 10-desacetyl paclitaxel Related Classifications
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