8α-Tigloyloxyhirsutinolide 13-O-acetate (Synonyms: 8αTGH)

Name: 8α-Tigloyloxyhirsutinolide 13-O-acetate
Brand: MedChemExpress
SKU: HY-138071
Rating: 4.5 (0 reviews)

製品番号: HY-138071 純度: 95.0%: Data Sheet SDS COA 取扱説明書
FAQs
Technical Support

8α-Tigloyloxyhirsutinolide 13-O-acetate (8αTGH) is a potent and orally active STAT3 inhibitor. 8α-Tigloyloxyhirsutinolide 13-O-acetate induces early oxidative stress and pyroptosis, and late DNA damage, cell cycle arrest, apoptosis in the TNBC cells. 8α-Tigloyloxyhirsutinolide 13-O-acetate suppresses tumor cell growth in vitro and tumor growth in vivo.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

8α-Tigloyloxyhirsutinolide 13-O-acetate 構造式

CAS 番号 : 83182-58-5

容量	価格（税別）	在庫状況	数量
1 mg	$550	在庫あり	Estimated Time of Arrival: December 31
5 mg	$1380	在庫あり	Estimated Time of Arrival: December 31
10 mg		お問い合わせ
50 mg		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 0 publication(s) in Google Scholar

STAT アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

STAT3 STAT5 STAT6 STAT1

Bcl-2 Family アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim BNIP3 Bad

TrxR アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

TrxR1 TrxR2

生物活性
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

IC₅₀ & Target

STAT3

Bcl-2

Bcl-xL

Mcl-1

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	IC₅₀	0.6 μM Compound: 4	Inhibition of TNFalpha-induced NF-kappaB activity expressed in human HEK 293 cells after 6 hrs by luciferase reporter gene assay Inhibition of TNFalpha-induced NF-kappaB activity expressed in human HEK 293 cells after 6 hrs by luciferase reporter gene assay	[PMID: 22850207]
MCF7	IC₅₀	10.4 μM Compound: 6	Antiproliferative activity against human MCF7 cells for 72 hrs by cyquant assay Antiproliferative activity against human MCF7 cells for 72 hrs by cyquant assay	[PMID: 26331426]
MDA-MB-231	IC₅₀	1.8 μM Compound: 6	Antiproliferative activity against human MDA-MB-231 cells for 72 hrs by cyquant assay Antiproliferative activity against human MDA-MB-231 cells for 72 hrs by cyquant assay	[PMID: 26331426]
NIH3T3	IC₅₀	2 μM Compound: 6	Cytotoxicity against mouse NIH/3T3 cells after 72 hrs by cyquant cells Cytotoxicity against mouse NIH/3T3 cells after 72 hrs by cyquant cells	[PMID: 26331426]
RAW264.7	IC₅₀	2 μM Compound: 4	Antiinflammatory activity against LPS-induced NO production in mouse RAW264.7 cells assessed as reduction in nitrite level preincubated for 15 mins prior to LPS treatment by Griess method Antiinflammatory activity against LPS-induced NO production in mouse RAW264.7 cells assessed as reduction in nitrite level preincubated for 15 mins prior to LPS treatment by Griess method	[PMID: 22850207]
SF-295	IC₅₀	7.5 μM Compound: 6	Antiproliferative activity against human SF295 cells for 72 hrs by cyquant assay Antiproliferative activity against human SF295 cells for 72 hrs by cyquant assay	[PMID: 26331426]
U-251	IC₅₀	1.7 μM Compound: 6	Antiproliferative activity against human U251MG cells for 72 hrs by cyquant assay Antiproliferative activity against human U251MG cells for 72 hrs by cyquant assay	[PMID: 26331426]
U-251	IC₅₀	2.2 μM Compound: 6	Inhibition of Stat3 dimer DNA binding activity in human U251MG cells nuclear extract after 1.5 hrs by EMSA using radiolabeled probe hSIE Inhibition of Stat3 dimer DNA binding activity in human U251MG cells nuclear extract after 1.5 hrs by EMSA using radiolabeled probe hSIE	[PMID: 26331426]
U-373MG ATCC	IC₅₀	2.3 μM Compound: 6	Inhibition of Stat3 dimer DNA binding activity in human U373MG cells nuclear extract after 1.5 hrs by EMSA using radiolabeled probe hSIE Inhibition of Stat3 dimer DNA binding activity in human U373MG cells nuclear extract after 1.5 hrs by EMSA using radiolabeled probe hSIE	[PMID: 26331426]
U-373MG ATCC	IC₅₀	3.5 μM Compound: 6	Antiproliferative activity against human U373MG cells for 72 hrs by cyquant assay Antiproliferative activity against human U373MG cells for 72 hrs by cyquant assay	[PMID: 26331426]

体外実験

8α-Tigloyloxyhirsutinolide 13-O-acetate (R001) (0-30 μM, 72 h) dose-dependently suppresses the viable cell numbers of the human cancer lines^[1].
8α-Tigloyloxyhirsutinolide 13-O-acetate (0-10 μM, 0-24 h) leads to early pyroptosis and late DNA damage, cell cycle arrest, and apoptosis only in the TNBC cells^[1].
8α-Tigloyloxyhirsutinolide 13-O-acetate (0-20 μM, 0-48 h) promotes ROS induction in triple-negative breast cancer cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	Multiple human cancer lines, normal human breast epithelial cells and normal human brain microvascular endothelial cells (HBMEC)
Concentration:	0, 1, 2.5, 5, 10, 20, 30 μM
Incubation Time:	72 h
Result:	Dose-dependently suppressed the viable cell numbers of the human cancer lines, MDA-MB-468, MDA-MB-231, Panc-1, A549, DU145, HCC1937, and MDA-MB-436 cells with IC₅₀ values of 2.3, 4.4, 4.3, 5.2, 5.8, 6.3, and 7.1 µM, respectively, compared to much weaker effects on the normal human breast epithelial cells, MCF-10A or HBMEC, with IC₅₀ of 23.9 or 14.2 µM, respectively.

Western Blot Analysis^[1]

Cell Line:	NIH3T3/v-Src fibroblasts, MDA-MB-231, MDA-MB-468, or MCF-10A cells
Concentration:	0, 2, 5, 10, 20 μM
Incubation Time:	30 min, 3 h, 24 h
Result:	Suppressed STAT3:STAT3 DNA-binding activity, with IC₅₀ of 5 µM. Showed the inhibition of STAT3 Tyr phosphorylation in time- and dose-dependent manner, while phospho-Ser-STAT3 (pS727-STAT3), pY1068EGFR, and pY-Jak2 were largely unaffected. Attenuated the expression of STAT3 downstream target genes, including c-Myc, Mcl-1, Bcl-2, Bcl-xL, and vascular endothelial growth factor (VEGF) in MDA-MB-468 and MDA-MB-231 cells.

Apoptosis Analysis^[1]

Cell Line:	Triple-negative breast cancer (TNBC), MDA-MB-231 and MDA-MB-468 cells, or normal human breast epithelial cells, MCF-10A
Concentration:	0, 2.5, 5 or 10 µM
Incubation Time:	6 or 24 h
Result:	Showed no significant induction of early apoptosis at 6 h, while the evidence of extensive cell death of 56.2% occurred at later time (24 h), with cleavage of poly (ADP-ribose) polymerase (PARP) and caspase 3 at 24-48 h.

体内実験

8α-Tigloyloxyhirsutinolide 13-O-acetate (5 mg/kg, Oral gavage, 5 times per week for 75 days) suppresses tumor growth in mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Five-week-old female athymic nude mice (injected subcutaneously in the right flank area with MDA-MB-468 cells in 100 μL PBS)
Dosage:	5 mg/kg
Administration:	Oral gavage, every day, 5 times per week for 75 days
Result:	Inhibited MDA-MB-468 xenografts growth in mice, with reduced pY705-STAT3, G6PD, TrxR1, and GSH levels.

分子量

420.45

分子式

C₂₂H₂₈O₈

CAS 番号

83182-58-5

Appearance

Solid

Color

White to off-white

SMILES

O=C(/C(C)=C/C)O[C@@H](C[C@H]1C)C(/C(OC2=O)=C\[C@](O[C@]13O)(CC3)C)=C2COC(C)=O

Structure Classification

Initial Source

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

純度とドキュメンテーション

純度: 95.0%

Select Batch:

データシート (283 KB) SDS (252 KB)

COA (262 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Zhu Y, et al. Natural product preferentially targets redox and metabolic adaptations and aberrantly active STAT3 to inhibit breast tumor growth in vivo. Cell Death Dis. 2022 Dec 6;13(12):1022. [Content Brief]

[2]. A Bardón, et al. Glaucolides and related sesquiterpene lactones fromVernonia nudiflora and Chrysolaena propinqua. Phytochemistry, 1992, 31(2):609-613.

8α-Tigloyloxyhirsutinolide 13-O-acetate Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

最近チェックした製品:

製品名

カスタマ需要量 *

お名前 *

タイトル

メールアドレス *

電話番号 *

デパートメント

組纖名 *

市区町村

都道府県

国或いは地域 *

必ず会社名を記載ください。個人への返信は行いません。

備考

製品名

Lot #

お名前 *

メールアドレス *

電話番号 *

部門 *

組纖名 *

国或いは地域 *

備考

8α-Tigloyloxyhirsutinolide 13-O-acetate (Synonyms: 8αTGH)

カスタマーレビュー

おすすめ

•関連小分子:

•関連タンパク質:

STAT アイソフォーム固有の製品をすべて表示:

Bcl-2 Family アイソフォーム固有の製品をすべて表示:

TrxR アイソフォーム固有の製品をすべて表示:

生物活性

純度とドキュメンテーション

参考文献

カスタマーレビュー

8α-Tigloyloxyhirsutinolide 13-O-acetate Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

最近チェックした製品:

お名前
メール *

	Sorry, but the email address you supplied was invalid.

8α-Tigloyloxyhirsutinolide 13-O-acetate (Synonyms: 8αTGH)

カスタマーレビュー

8α-Tigloyloxyhirsutinolide 13-O-acetate 関連抗体

おすすめ

•関連小分子:

•関連タンパク質:

STAT アイソフォーム固有の製品をすべて表示:

Bcl-2 Family アイソフォーム固有の製品をすべて表示:

TrxR アイソフォーム固有の製品をすべて表示:

生物活性

純度とドキュメンテーション

参考文献

カスタマーレビュー

8α-Tigloyloxyhirsutinolide 13-O-acetate 関連抗体

8α-Tigloyloxyhirsutinolide 13-O-acetate Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

最近チェックした製品:

カスタマーレビュー

Request for HNMR Report

Request for HNMR Report