2. MAPK/ERK Pathway Stem Cell/Wnt
  3. ERK
  4. AG126

AG126  (Synonyms: Tyrphostin AG126)

Cat. No.: HY-108330 Purity: ≥98.0%
COA Handling Instructions

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AG126 is a tyrosine kinase inhibitor, can inhibit the phosphorylation of ERK1 and ERK2 at 25-50 μM. AG126 can be used in meiosis, mitosis, and postmitotic research.

For research use only. We do not sell to patients.

AG126 Chemical Structure

AG126 Chemical Structure

CAS No. : 118409-62-4

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 95 In-stock
Solution
10 mM * 1 mL in DMSO USD 95 In-stock
Solid
1 mg USD 40 In-stock
5 mg USD 84 In-stock
10 mg USD 144 In-stock
25 mg USD 312 In-stock
50 mg USD 564 In-stock
100 mg USD 1008 In-stock
200 mg   Get quote  
500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 2 publication(s) in Google Scholar

AG126 Related Antibodies

Top Publications Citing Use of Products

View All ERK Isoform Specific Products:

View All Isoforms
ERK ERK1 ERK2 ERK5 ERK8

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

AG126 is a tyrosine kinase inhibitor, can inhibit the phosphorylation of ERK1 and ERK2 at 25-50 μM. AG126 can be used in meiosis, mitosis, and postmitotic research[1][2][3][4].

IC50 & Target[1]

ERK2

 

In Vitro

AG126 (10 μM; overnight) increases the viability of ARPE-19 cells[2].
AG126 at concentrations higher than 10 μM show toxic to ARPE-19 cells and can enhance H2O2 toxicity[2].
AG126 (0.1-100 μM) inhibits VEGF-induced proliferation of BRMECs[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

AG126 Related Antibodies

Cell Viability Assay[2]

Cell Line: ARPE-19 cells
Concentration: 10 μM
Incubation Time: Overnight
Result: Increased the viability of ARPE-19 cells to 35-72% compared to the control.

Cell Proliferation Assay[4]

Cell Line: BRMECs
Concentration: 0.1-100 μM
Incubation Time:
Result: Inhibited VEGF-induced proliferation of BRMECs in a dose-dependent manner.
In Vivo

AG 126 (intraperitoneal injection; 1-10 mg/kg; 1 h and 6 h after Zymosan treatment) treatment attenuates the degree of multiple organ failure (MOF) associated with Zymosan-induced peritonitis in the rat[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Sprague-Dawley rats treated with Zymosan (500 mg/kg)[3]
Dosage: 10 mg/kg, 3 mg/kg or 1 mg/kg
Administration: Intraperitoneal injection; 10, 3, or 1 mg/kg; 1 h and 6 h after Zymosan treatment
Result: Attenuated the peritoneal exudation and the migration of polymorphonuclear cells caused by Zymosan in a dose-dependent fashion.
Attenuated the lung, liver, and intestinal injury.
Reduced the production of peroxynitrite and of pro-inflammatory cytokines TNF-alpha and IL-1beta.
Molecular Weight

215.17

Formula

C10H5N3O3
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

N#C/C(C#N)=C/C1=CC=C([N+]([O-])=O)C(O)=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (464.75 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.6475 mL 23.2374 mL 46.4749 mL
5 mM 0.9295 mL 4.6475 mL 9.2950 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (11.62 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: ≥98.0%

COA (248 KB) HNMR (233 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.6475 mL 23.2374 mL 46.4749 mL 116.1872 mL
5 mM 0.9295 mL 4.6475 mL 9.2950 mL 23.2374 mL
10 mM 0.4647 mL 2.3237 mL 4.6475 mL 11.6187 mL
15 mM 0.3098 mL 1.5492 mL 3.0983 mL 7.7458 mL
20 mM 0.2324 mL 1.1619 mL 2.3237 mL 5.8094 mL
25 mM 0.1859 mL 0.9295 mL 1.8590 mL 4.6475 mL
30 mM 0.1549 mL 0.7746 mL 1.5492 mL 3.8729 mL
40 mM 0.1162 mL 0.5809 mL 1.1619 mL 2.9047 mL
50 mM 0.0929 mL 0.4647 mL 0.9295 mL 2.3237 mL
60 mM 0.0775 mL 0.3873 mL 0.7746 mL 1.9365 mL
80 mM 0.0581 mL 0.2905 mL 0.5809 mL 1.4523 mL
100 mM 0.0465 mL 0.2324 mL 0.4647 mL 1.1619 mL
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AG126 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AG126118409-62-4Tyrphostin AG126AG 126AG-126Tyrphostin AG 126Tyrphostin AG-126ERKExtracellular signal regulated kinasesARPE-19BRMECsInhibitorinhibitorinhibit

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