1. Apoptosis
  2. TNF Receptor
  3. Adalimumab

Adalimumab (Synonyms: Anti-Human TNF-alpha, Human Antibody)

Cat. No.: HY-P9908 Purity: 98.12%
Handling Instructions

Adalimumab is a human monoclonal IgG1 antibody targeting tumour necrosis factor α (TNF-α).

For research use only. We do not sell to patients.

Adalimumab Chemical Structure

Adalimumab Chemical Structure

CAS No. : 331731-18-1

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Description

Adalimumab is a human monoclonal IgG1 antibody targeting tumour necrosis factor α (TNF-α).

IC50 & Target

TNF-α[1]

In Vitro

Treatment of HUVECs with Adalimumab for up to 12 hours shows no adverse effect on their cell viability, even with a 10-fold higher concentration of Adalimumab than used in this study[2].

In Vivo

The elimination half-life (t1/2) of Adalimumab is long (~20 days)[1]. The enriched fluorescent-labelled adalimumab is observed mainly in major Oil red O-positive lesions documenting good tissue penetration of the drug into the atherosclerotic plaque, without background fluorescence of the vessel wall after vehicle control injection into hypercholesterolemic Ldlr-/- mice[2].

Clinical Trial
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

References
Cell Assay
[2]

1×104 HUVECs are plated per well of 96 well plate in EGM. After reaching sub-confluency cells are cultured in different concentration of Adalimumab (0.01-10 μg/mL), IgG isotype control (10 μg/mL) and 0.1% Triton-X as positive control in 5% CO2 at 37°C. After 12 hours, 10% Alamar blue is added to each well and kept in incubator for another 6 hours. Absorbance of oxidized alamar blue is measured at 600 nm on Infinite M200 pro plate reader. All measurements are performed in triplicate[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[2]

Mice[2]
In order to analyze the vascular distribution of Adalimumab, the substance is labelled using the DyLight 549 Microscale Antibody Labeling Kit. Briefly, one vial of DyLight 549 Reagent is dissolved in 1 mg Adalimumab solution (in PBS) and purified with a resin spin column to remove excess fluor. Hypercholesterolemic Ldlr-/- mice are injected with fluorophore-labelled Adalimumab (8.0 mg/kg in 200 μL PBS i.p.) for two consecutive days and sacrificed 12 hours after last injection. Vehicle injection (200 μL PBS) is used as control.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

145425.42

CAS No.

331731-18-1

SMILES

[Adalimumab]

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Adalimumab
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Adalimumab

Cat. No.: HY-P9908