Adalimumab
Based on 16 publication(s) in Google Scholar
Adalimumab is a human monoclonal IgG1 antibody targeting tumour necrosis factor α (TNF-α).
For research use only. We do not sell to patients.
- Purity: 99.27%
- CAS No.: 331731-18-1
- Molecular Weight:145.43 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Adalimumab
More- Nat Immunol. 2026 Jun;27(6):1184-1196. [Abstract]
- Adv Mater. 2025 Dec 12:e13952. [Abstract]
- Adv Sci (Weinh). 2024 Oct 23:e2404559. [Abstract]
- J Clin Invest. 2025 Jan 16;135(2):e178198. [Abstract]
- Cell Mol Life Sci. 2024 May 28;81(1):240. [Abstract]
- Anal Chim Acta. 2021 Apr 15:1154:338306. [Abstract]
- Xenotransplantation. 2019 Nov;26(6):e12536. [Abstract]
- Bioconjug Chem. 2025 Aug 21. [Abstract]
- BMC Oral Health. 2024 Jan 18;24(1):107. [Abstract]
- Vet Microbiol. 2023 Jun:281:109730. [Abstract]
- bioRxiv. 2026 May 29.
- bioRxiv. 2026 Apr 30.
- bioRxiv. 2026 Apr 23.
- bioRxiv. 2025 Aug 23:2025.08.19.670635. [Abstract]
- bioRxiv. 2024 Nov 4:2024.10.08.617155. [Abstract]
- SSRN. 2024 Mar 6.
Customer Validation & Images
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ELISA
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Cell Imaging/Staining
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Flow Cytometry
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RT-PCR
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Bio/Physico-chemical Assay
Biological Activity
Human IgG1 kappa
Human
TNFSF2/TNFa
Treatment of HUVECs with Adalimumab for up to 12 hours shows no adverse effect on their cell viability, even with a 10-fold higher concentration of Adalimumab than used in this study[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
The enriched fluorescent-labelled adalimumab is observed mainly in major Oil red O-positive lesions documenting good tissue penetration of the drug into the atherosclerotic plaque, without background fluorescence of the vessel wall after vehicle control injection into hypercholesterolemic Ldlr-/- mice[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
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Human IgG1 kappa
ELISA, FACS, Functional assay
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Loaded Adalimumab on AHC2 biosensor, can bind TNF-alpha/TNFSF2 Protein, Human (177a.a, His, HY-P700291) with an affinity constant of 2.247E-10 M as determined in BLI assay. -
Immobilized TNF-alpha/TNFSF2 Protein, Human (HY-P7058) can bind Adalimumab. The EC50 for this effect is 6.753 ng/mL. -
Flow Cytometry analysis of THP-1 cells with Adalimumab (HY-P9908, red) with Adalimumab (anti-Cytokeratin 19) (HY-P9908). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
Chemical Information
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CAS No. 331731-18-1
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Appearance Liquid
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Molecular Weight 145.43 kDa
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Color Colorless to light yellow
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SMILES
[Adalimumab]
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Synonyms
Anti-Human TNF-alpha, Human Antibody; ABBV-3373 Antibody
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Shipping
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (16)
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Journal Impact Factor
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Most Recent
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Nat Immunol
A spatially coordinated keratinocyte-fibroblast circuit recruits MMP9+ myeloid cells to drive type I interferon-driven inflammation in photosensitive autoimmunity. [Abstract]2026 Jun;27(6):1184-1196. PMID: 42032302 -
Adv Mater
3D Bioprinted Human Synovium-Cartilage Models Mimic Rheumatoid Arthritis Microenvironment and Recapitulate In Vivo Therapeutic Responses. [Abstract]2025 Dec 12:e13952. PMID: 41388589
Adalimumab purchased from MedChemExpress. Usage Cited in: Adv Mater. 2025 Dec 12:e13952. [Abstract]
Inhibitory dose-response curves to determine the IC50 for Adalimumab (5-20 μg/mL; 72 h) on secretion of TNF-α in the established rheumatoid arthritis model.
Adalimumab purchased from MedChemExpress. Usage Cited in: Adv Mater. 2025 Dec 12:e13952. [Abstract]
Analysis of the ROS levels in the synovium part and cartilage part treated with Methotrexate (MTX), Adalimumab (ADA; 5-20 μg/mL; 72 h), and the combination of MTX and ADA.
Adalimumab purchased from MedChemExpress. Usage Cited in: Adv Mater. 2025 Dec 12:e13952. [Abstract]
Drug testing in the 3D coculture RA model with T and B cells. The expression of Ki 67 and TNF-α on T and B cells evaluated by flow cytometer. RA, M1 Mφ incorporated 3D model that cocultured with T and B cells. RA+MTX (2 μM), RA model treated with Methotrexate. RA+ADA (5 μg/mL), RA model treated with Adalimumab. RA+MTX+ADA, RA model treated with Methotrexate and Adalimumab.
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Adv Sci (Weinh)
Free Electron Density Gradients Enhanced Biosensor for Ultrasensitive and Accurate Affinity Assessment of the Immunotherapy Drugs. [Abstract]2024 Oct 23:e2404559. PMID: 39443825
Adalimumab purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Oct 23:e2404559. [Abstract]
The real-time assessment data of the affinity between TNF-α protein and Adalimumab (2.5-80 nM) detected by the FED-MSPR biosensor.
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J Clin Invest
Inhibition of aortic CX3CR1+ macrophages mitigates thoracic aortic aneurysm progression in Marfan syndrome in mice. [Abstract]2025 Jan 16;135(2):e178198. PMID: 39817456
Adalimumab purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2025 Jan 16;135(2):e178198. [Abstract]
Real-time PCR of gene expression in MFS patient-specific iPSC-derived VSMCs with conditional media treatment in the presence of Adalimumab (10 μg/mL; 24 h) or IgG.
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Cell Mol Life Sci
Disruption of pulmonary microvascular endothelial barrier by dysregulated claudin-8 and claudin-4: uncovered mechanisms in porcine reproductive and respiratory syndrome virus infection. [Abstract]2024 May 28;81(1):240. PMID: 38806818 -
Anal Chim Acta
Calibration for quantitative Fc-glycosylation analysis of therapeutic IgG1-type monoclonal antibodies by using glycopeptide standards. [Abstract]2021 Apr 15:1154:338306. PMID: 33736796 -
Xenotransplantation
Selective inhibition of cyclooxygenase-2 protects porcine aortic endothelial cells from human antibody-mediated complement-dependent cytotoxicity. [Abstract]2019 Nov;26(6):e12536. PMID: 31724835 -
Bioconjug Chem
Resin-Supported Site-Specific Antibody Conjugation Method Leads to Antibody-Drug Conjugates with Retained Efficacy and Improved Stability. [Abstract]2025 Aug 21. PMID: 40839643 -
BMC Oral Health
M2 macrophages-derived exosomes regulate osteoclast differentiation by the CSF2/TNF-α axis. [Abstract]2024 Jan 18;24(1):107. PMID: 38238696 -
Vet Microbiol
PRRSV-induced inflammation in pulmonary intravascular macrophages (PIMs) and pulmonary alveolar macrophages (PAMs) contributes to endothelial barrier function injury. [Abstract]2023 Jun:281:109730. PMID: 37068404 -
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bioRxiv
A Spatially Coordinated Keratinocyte-Fibroblast Circuit Recruits MMP9+ Myeloid Cells to Drive IFN-I-Driven Inflammation in Photosensitive Autoimmunity. [Abstract]2025 Aug 23:2025.08.19.670635. PMID: 40894544 -
bioRxiv
2024 Nov 4:2024.10.08.617155. PMID: 39415993 -
Protocol
1×104 HUVECs are plated per well of 96 well plate in EGM. After reaching sub-confluency cells are cultured in different concentration of Adalimumab (0.01-10 μg/mL), IgG isotype control (10 μg/mL) and 0.1% Triton-X as positive control in 5% CO2 at 37°C. After 12 hours, 10% Alamar blue is added to each well and kept in incubator for another 6 hours. Absorbance of oxidized alamar blue is measured at 600 nm on Infinite M200 pro plate reader. All measurements are performed in triplicate[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Mice[2]
In order to analyze the vascular distribution of Adalimumab, the substance is labelled using the DyLight 549 Microscale Antibody Labeling Kit. Briefly, one vial of DyLight 549 Reagent is dissolved in 1 mg Adalimumab solution (in PBS) and purified with a resin spin column to remove excess fluor. Hypercholesterolemic Ldlr-/- mice are injected with fluorophore-labelled Adalimumab (8.0 mg/kg in 200 μL PBS i.p.) for two consecutive days and sacrificed 12 hours after last injection. Vehicle injection (200 μL PBS) is used as control.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (263 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Ternant D, et al. Pharmacokinetics and concentration-effect relationship of adalimumab in rheumatoid arthritis. Br J Clin Pharmacol. 2015 Feb;79(2):286-97. [Content Brief]
[2]. Oberoi R, et al. Targeting Tumor Necrosis Factor-α with Adalimumab: Effects on Endothelial Activation and Monocyte Adhesion. PLoS One. 2016 Jul 28;11(7):e0160145. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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