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  3. TNF Receptor Bacterial
  4. Adalimumab

Adalimumab  (Synonyms: Anti-Human TNF-alpha, Human Antibody; ABBV-3373 Antibody)

Cat. No.: HY-P9908 Purity: 99.27%
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Adalimumab is a human monoclonal IgG1 antibody targeting tumour necrosis factor α (TNF-α).

For research use only. We do not sell to patients.

CAS No. : 331731-18-1

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Customer Review

Based on 12 publication(s) in Google Scholar

Adalimumab Featured Recommendations:

Adalimumab Related Antibodies

Top Publications Citing Use of Products

    Adalimumab purchased from MedChemExpress. Usage Cited in: Adv Mater. 2025 Dec 12:e13952.  [Abstract]

    Inhibitory dose-response curves to determine the IC50 for Adalimumab (5-20 μg/mL; 72 h) on secretion of TNF-α in the established rheumatoid arthritis model.

    Adalimumab purchased from MedChemExpress. Usage Cited in: Adv Mater. 2025 Dec 12:e13952.  [Abstract]

    Analysis of the ROS levels in the synovium part and cartilage part treated with Methotrexate (MTX), Adalimumab (ADA; 5-20 μg/mL; 72 h), and the combination of MTX and ADA.

    Adalimumab purchased from MedChemExpress. Usage Cited in: Adv Mater. 2025 Dec 12:e13952.  [Abstract]

    Drug testing in the 3D coculture RA model with T and B cells. The expression of Ki 67 and TNF-α on T and B cells evaluated by flow cytometer. RA, M1 Mφ incorporated 3D model that cocultured with T and B cells. RA+MTX (2 μM), RA model treated with Methotrexate. RA+ADA (5 μg/mL), RA model treated with Adalimumab. RA+MTX+ADA, RA model treated with Methotrexate and Adalimumab.

    Adalimumab purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2025 Jan 16;135(2):e178198.  [Abstract]

    Real-time PCR of gene expression in MFS patient-specific iPSC-derived VSMCs with conditional media treatment in the presence of Adalimumab (10 μg/mL; 24 h) or IgG.

    Adalimumab purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Oct 23:e2404559.  [Abstract]

    The real-time assessment data of the affinity between TNF-α protein and Adalimumab (2.5-80 nM) detected by the FED-MSPR biosensor.

    View All TNF Receptor Isoform Specific Products:

    View All Isoforms
    TNFRSF1A/CD120a TNFRSF3/CD18 TNFRSF4 TNFRSF5/CD40 TNFRSF6/Fas/CD95 TNFRSF7/CD27 TNFRSF8/CD30 TNFRSF9/4-1BB/CD137 TNFRSF10B/DR5/CD262 TNFRSF12A/TWEAK TNFRSF16/NGF Receptor/CD271 TNFRSF18/GITR/CD357

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Adalimumab is a human monoclonal IgG1 antibody targeting tumour necrosis factor α (TNF-α).

    Isotype

    Human IgG1 kappa
    Recommend Isotype Controls
    Species Reactivity

    Human
    IC50 & Target

    TNFSF2/TNFa
    In Vitro

    Treatment of HUVECs with Adalimumab for up to 12 hours shows no adverse effect on their cell viability, even with a 10-fold higher concentration of Adalimumab than used in this study[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Adalimumab Related Antibodies
    In Vivo

    The elimination half-life (t1/2) of Adalimumab is long (~20 days)[1].
    The enriched fluorescent-labelled adalimumab is observed mainly in major Oil red O-positive lesions documenting good tissue penetration of the drug into the atherosclerotic plaque, without background fluorescence of the vessel wall after vehicle control injection into hypercholesterolemic Ldlr-/- mice[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    Gene ID

    7124  [NCBI]

    Accession
    Application

    ELISA, FACS, Functional assay
    Conjugated

    Unconjugated
    Reconsititution

    The product can be reconstituted/diluted with sterile PBS or saline.
    Molecular Weight

    145.43 kDa

    CAS No.
    Appearance

    Liquid

    Color

    Colorless to light yellow

    SMILES

    [Adalimumab]
    Shipping

    Shipping with dry ice.
    Formulation

    Please refer to the lot-specific COA for specific buffer information.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Format
    • Human IgG1 kappa
    Biological Activity
    • Loaded Adalimumab on AHC2 biosensor, can bind TNF-alpha/TNFSF2 Protein, Human (177a.a, His, HY-P700291) with an affinity constant of 2.247E-10 M as determined in BLI assay.
    • Immobilized TNF-alpha/TNFSF2 Protein, Human (HY-P7058) can bind Adalimumab. The EC50 for this effect is 6.753 ng/mL.
    • Flow Cytometry analysis of THP-1 cells with Adalimumab (HY-P9908, red) with Adalimumab (anti-Cytokeratin 19) (HY-P9908). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
    Purity & Documentation

    Purity: 99.62%

    SDS (252 KB)

    COA (232 KB) SDS-PAGE (81 KB) SEC-HPLC (110 KB)

    Inhibitory Antibodies User Guide (603 KB)
    References
    Cell Assay
    [2]

    1×104 HUVECs are plated per well of 96 well plate in EGM. After reaching sub-confluency cells are cultured in different concentration of Adalimumab (0.01-10 μg/mL), IgG isotype control (10 μg/mL) and 0.1% Triton-X as positive control in 5% CO2 at 37°C. After 12 hours, 10% Alamar blue is added to each well and kept in incubator for another 6 hours. Absorbance of oxidized alamar blue is measured at 600 nm on Infinite M200 pro plate reader. All measurements are performed in triplicate[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [2]

    Mice[2]
    In order to analyze the vascular distribution of Adalimumab, the substance is labelled using the DyLight 549 Microscale Antibody Labeling Kit. Briefly, one vial of DyLight 549 Reagent is dissolved in 1 mg Adalimumab solution (in PBS) and purified with a resin spin column to remove excess fluor. Hypercholesterolemic Ldlr-/- mice are injected with fluorophore-labelled Adalimumab (8.0 mg/kg in 200 μL PBS i.p.) for two consecutive days and sacrificed 12 hours after last injection. Vehicle injection (200 μL PBS) is used as control.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References
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    • If you have published this work, please enter the PubMed ID.
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    Adalimumab Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Adalimumab331731-18-1Anti-Human TNF-alpha, Human Antibody ABBV-3373 AntibodyTNF ReceptorBacterialTumor Necrosis Factor ReceptorTNFRInhibitorinhibitorinhibit

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